4E34: CFTR Associated Ligand (CAL) PDZ domain

Crystal structure of CFTR Associated Ligand (CAL) PDZ domain bound to iCAL36 (ANSRWPTSII) peptide. Determined by X-ray diffraction at 1.4 Å resolution. Released 26 Dec 2012.

Method: X-ray diffraction
Resolution: 1.4 Å
Organism: Homo sapiens
Chains: 4
Atoms: 1,770
Mol. weight: 21.27 kDa
Released: 26 Dec 2012

Explore 4E34 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4E34 contains 6 α-helices and 15 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 3 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	287-292	6	1
β-strand	297	1	2
β-strand	301-306	6	1
α-helix	307-309	3
β-strand	311-318	8	1
α-helix	323-327	5
β-strand	334-339	6	1
β-strand	342-343	2	1
α-helix	349-358	10
β-strand	362-369	8	1

Chain B: 3 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	287-293	7	2
β-strand	301-306	6	2
α-helix	307-309	3
β-strand	311-318	8	2
α-helix	323-327	5
β-strand	335-339	5	2
β-strand	342-343	2	2
α-helix	349-358	10
β-strand	361-368	8	2

Chain C: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	7-9	3	1

Chain D: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	8-9	2	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Golgi-associated PDZ and coiled-coil motif-containing protein	A, B	protein	87	Homo sapiens	Q9HD26 (AlphaFold model)
decameric peptide, iCAL36	C, D	protein	10

Sequence of entity 1 (A, B), FASTA

>4E34_1 Golgi-associated PDZ and coiled-coil motif-containing protein (chains A, B)
GPIRKVLLLKEDHEGLGISITGGKEHGVPILISEIHPGQPADRCGGLHVGDAILAVNGVN
LRDTKHKEAVTILSQQRGEIEFEVVYV

Sequence of entity 2 (C, D), FASTA

>4E34_2 decameric peptide, iCAL36 (chains C, D)
ANSRWPTSII

Primary citation

Stereochemical Determinants of C-terminal Specificity in PDZ Peptide-binding Domains: A NOVEL CONTRIBUTION OF THE CARBOXYLATE-BINDING LOOP. Amacher, J.F., Cushing, P.R., Bahl, C.D. et al. J Biol Chem (2013) 288:5114-5126. DOI 10.1074/jbc.M112.401588 · PubMed

Other PDB entries of the same protein (UniProt Q9HD26 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4JOK 1.09 Å, CFTR Associated Ligand (CAL) PDZ domain bound to peptide Y-iCAL36 (ANSRYPTSII)
4JOE 1.14 Å, CFTR Associated Ligand (CAL) PDZ domain bound to peptide A-iCAL36 (ANSRAPTSII)
4JOF 1.2 Å, CFTR Associated Ligand (CAL) PDZ domain bound to peptide L-iCAL36 (ANSRLPTSII)
4JOJ 1.2 Å, CFTR Associated Ligand (CAL) domain bound to peptide F-iCAL36 (ANSRFPTSII)
4NMP 1.3 Å, CFTR Associated Ligand (CAL) PDZ domain bound to peptide iCAL36(Ac-K-3) (ANSRWP[Ac-K]SII)
4JOR 1.34 Å, CFTR Associated Ligand (CAL) PDZ domain bound to HPV18 E6 oncoprotein C-terminal peptide…
7JZQ 1.35 Å, CFTR Associated Ligand (CAL) PDZ domain bound to peptidomimetic LyCALPMB
5K4F 1.36 Å, CAL PDZ mutant C319A with a peptide
4E35 1.4 Å, Crystal structure of CFTR Associated Ligand (CAL) PDZ domain bound to iCAL36-L…
4NMO 1.4 Å, CFTR Associated Ligand (CAL) PDZ domain bound to peptide iCAL36(Ac-K-1)(ANSRWPTS[Ac-K]I)
4NMQ 1.4 Å, CFTR Associated Ligand (CAL) PDZ domain bound to peptide iCAL36(Ac-K-4) (ANSRW[Ac-K]TSII)
4NMT 1.4 Å, CFTR Associated Ligand (CAL) PDZ domain bound to peptide iCAL36(TFA-K-1)…

Browse structure collections

About this viewer

MolViewer shows 4E34 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.