4EOI: Cyclin-dependent kinase 2

Thr 160 phosphorylated CDK2 K89D, Q131E - human cyclin A3 complex with the inhibitor RO3306. Determined by X-ray diffraction at 2.0 Å resolution. Released 6 Feb 2013.

Method
X-ray diffraction
Resolution
2.0 Å
Organism
Homo sapiens
Chains
4
Atoms
9,466
Mol. weight
128.51 kDa
Ligands
SGM, 1RO
Released
6 Feb 2013

Explore 4EOI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4EOI contains 74 α-helices and 24 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 18 helices, 11 β-strands

ElementResiduesLengthSheet
α-helix1-33
β-strand4-1291
β-strand17-2371
β-strand29-3681
α-helix46-5712
β-strand6312
β-strand66-7161
β-strand75-8171
β-strand85-8622
α-helix87-937
α-helix101-12020
β-strand123-12423
α-helix130-1323
β-strand133-13532
β-strand141-14332
α-helix146-1483
β-strand150-15123
α-helix166-1683
α-helix171-1744
α-helix183-19816
α-helix208-21912
α-helix230-2323
α-helix240-2423
α-helix248-2514
α-helix257-26610
α-helix275-2762
α-helix277-2804
α-helix284-2863
α-helix292-2954
Chain B: 20 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix179-19214
α-helix199-2024
α-helix208-22417
α-helix229-24315
α-helix250-2523
α-helix253-26816
α-helix272-2743
α-helix275-2806
α-helix288-30114
α-helix311-3199
α-helix327-34216
α-helix344-3474
α-helix352-36716
α-helix374-3807
α-helix384-40017
α-helix401-4033
α-helix408-4125
α-helix416-4183
α-helix421-4233
α-helix425-4273
Chain C: 16 helices, 13 β-strands
ElementResiduesLengthSheet
β-strand4-1294
β-strand17-2374
β-strand29-3684
β-strand3815
β-strand4315
α-helix46-5712
β-strand6316
β-strand66-7054
β-strand75-8174
β-strand85-8626
α-helix87-937
α-helix101-12020
β-strand123-12427
α-helix130-1323
β-strand133-13536
β-strand141-14336
α-helix146-1483
β-strand150-15127
α-helix166-1683
α-helix171-1744
α-helix183-19816
α-helix208-21912
α-helix243-2475
α-helix248-2503
α-helix257-26610
α-helix275-2762
α-helix277-2804
α-helix284-2863
α-helix292-2954
Chain D: 20 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix179-19214
α-helix194-1963
α-helix200-2023
α-helix208-22417
α-helix229-24517
α-helix250-2523
α-helix253-26816
α-helix272-2743
α-helix275-2806
α-helix288-30114
α-helix311-3199
α-helix327-34216
α-helix344-3474
α-helix352-36716
α-helix374-3807
α-helix388-40013
α-helix401-4033
α-helix408-4125
α-helix416-4183
α-helix425-4273

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Cyclin-dependent kinase 2A, Cprotein299Homo sapiensP24941 (AlphaFold model)
Cyclin-A2B, Dprotein258Homo sapiensP20248 (AlphaFold model)
Sequence of entity 1 (A, C), FASTA
>4EOI_1 Cyclin-dependent kinase 2 (chains A, C)
SMENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELN
HPNIVKLLDVIHTENKLYLVFEFLHQDLKDFMDASALTGIPLPLIKSYLFQLLQGLAFCH
SHRVLHRDLKPENLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKY
YSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPS
FPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL
Sequence of entity 2 (B, D), FASTA
>4EOI_2 Cyclin-A2 (chains B, D)
VPDYHEDIHTYLREMEVKCKPKVGYMKKQPDITNSMRAILVDWLVEVGEEYKLQNETLHL
AVNYIDRFLSSMSVLRGKLQLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQVLRM
EHLVLKVLTFDLAAPTVNQFLTQYFLHQQPANCKVESLAMFLGELSLIDADPYLKYLPSV
IAGAAFHLALYTVTGQSWPESLIRKTGYTLESLKPCLMDLHQTYLKAPQHAQQSIREKYK
NSKYHGVSLLNPPETLNL

Ligands and cofactors

IDNameFormulaCopies
SGMMonothioglycerolC3 H8 O2 S2
1RO(5E)-5-(quinolin-6-ylmethylidene)-2-[(thiophen-2-ylmethyl)amino]-1,3-thiazol-4(…C18 H13 N3 O S22

Water and common crystallization additives (DMS) are not listed.

Primary citation

An integrated chemical biology approach provides insight into Cdk2 functional redundancy and inhibitor sensitivity. Echalier, A., Cot, E., Camasses, A. et al. Chem Biol (2012) 19:1028-1040. DOI 10.1016/j.chembiol.2012.06.015 · PubMed

Other PDB entries of the same protein (UniProt P24941 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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