4JML: TolB(P201C)-ColicinE9 TBE peptide(A33C) complex

Crystal structure of the TolB(P201C)-ColicinE9 TBE peptide(A33C) complex. Determined by X-ray diffraction at 2.0 Å resolution. Released 17 Jul 2013.

Method: X-ray diffraction
Resolution: 2.0 Å
Organism: Escherichia coli
Chains: 2
Atoms: 3,435
Mol. weight: 46.4 kDa
Ligands: CA
Released: 17 Jul 2013

Explore 4JML in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4JML contains 8 α-helices and 31 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 31 β-strands

Element	Residues	Length	Sheet
β-strand	35-37	3	1
β-strand	38-39	2	2
β-strand	43	1	2
α-helix	54-64	11
β-strand	68-70	3	1
α-helix	73-75	3
α-helix	83-85	3
α-helix	88-92	5
β-strand	98-106	9	2
β-strand	112-120	9	2
β-strand	128-136	9	2
α-helix	138-140	3
α-helix	141-157	17
β-strand	166-172	7	3
β-strand	180-186	7	3
β-strand	193-198	6	3
β-strand	202-207	6	4
β-strand	213-218	6	4
β-strand	225-230	6	4
β-strand	236-240	5	4
β-strand	246-251	6	5
β-strand	257-262	6	5
β-strand	269-274	6	5
β-strand	280-282	3	5
β-strand	290-295	6	6
β-strand	301-306	6	6
β-strand	313-318	6	6
β-strand	325-326	2	6
β-strand	333-339	7	7
β-strand	345-352	8	7
β-strand	355-362	8	7
β-strand	368-370	3	7
β-strand	378-382	5	8
β-strand	388-392	5	8
α-helix	395-398	4
β-strand	401-405	5	8
β-strand	411-413	3	8
α-helix	414-417	4
β-strand	420-426	7	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Protein TolB	A	protein	417	Escherichia coli	P0A855 (AlphaFold model)
Colicin-E9	E	protein	16	Escherichia coli	P09883 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>4JML_1 Protein TolB (chains A)
MVRIVIDSGVDSGRPIGVVPFQWAGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPG
SAQEVQPAAWSALGIDAVVVGQVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWL
RYAGHTASDEVFEKLTGIKGAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQC
LMSPAWSPDGSKLAYVTFESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFA
LSKTGSLNLYVMDLASGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNING
GAPQRITWEGSQNQDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPS
LAPNGTMVIYSSSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYLLEHHHHHH

Sequence of entity 2 (E), FASTA

>4JML_2 Colicin-E9 (chains E)
GCSDGSGWSSENNPWG

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	1

Primary citation

Intrinsically disordered protein threads through the bacterial outer-membrane porin OmpF. Housden, N.G., Hopper, J.T., Lukoyanova, N. et al. Science (2013) 340:1570-1574. DOI 10.1126/science.1237864 · PubMed

Other PDB entries of the same protein (UniProt P0A855 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2HQS 1.5 Å, Crystal structure of TolB/Pal complex
2W8B 1.86 Å, Crystal structure of processed TolB in complex with Pal
1CRZ 1.95 Å, Crystal structure of the E. Coli tolb protein
1C5K 2.0 Å, The structure of tolb, an essential component of the tol-dependent translocation system…
2IVZ 2.0 Å, Structure of TolB in complex with a peptide of the colicin E9 T- domain
3IAX 2.6 Å, The crystal structure of the TolB box of Colicin A in complex with TolB reveals…
7NST 3.7 Å, ColicinE9 partial translocation complex
7NSU 4.7 Å, ColicinE9 intact translocation complex
9A2L Model of E. coli TolB by in-cell photo-crosslinking MS and deep learning

Browse structure collections

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