4MAK: CRISPR-associated endoribonuclease Cas2

Crystal structure of a putative ssRNA endonuclease Cas2, CRISPR adaptation protein from E.coli. Determined by X-ray diffraction at 1.1 Å resolution. Released 4 Sept 2013.

Method: X-ray diffraction
Resolution: 1.1 Å
Organism: Escherichia coli
Chains: 2
Atoms: 1,413
Mol. weight: 21.16 kDa
Released: 4 Sept 2013

Explore 4MAK in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4MAK contains 4 α-helices and 10 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 2 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	1
α-helix	13-22	10
β-strand	24-27	4	1
β-strand	30-34	5	1
α-helix	37-50	14
β-strand	55-61	7	1
β-strand	68-73	6	1

Chain B: 2 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	2
α-helix	13-22	10
β-strand	24-27	4	2
β-strand	30-34	5	2
α-helix	37-50	14
β-strand	55-61	7	2
β-strand	68-74	7	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
CRISPR-associated endoribonuclease Cas2	A, B	protein	94	Escherichia coli	P45956 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>4MAK_1 CRISPR-associated endoribonuclease Cas2 (chains A, B)
MSMLVVVTENVPPRLRGRLAIWLLEVRAGVYVGDVSAKIREMIWEQIAGLAEEGNVVMAW
ATNTETGFEFQTFGLNRRTPVDLDGLRLVSFLPV

Primary citation

Crystal structure of a putative ssRNA endonuclease Cas2, CRISPR adaptation protein from E.coli. Nocek, B., Skarina, T., Brown, G. et al. To be published.

Other PDB entries of the same protein (UniProt P45956 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4P6I 2.3 Å, Crystal structure of the Cas1-Cas2 complex from Escherichia coli
5DLJ 2.6 Å, Crystal Structure of Cas-DNA-N1 complex
4QDL 2.7 Å, Crystal structure of E.coli Cas1-Cas2 complex
5DQZ 2.7 Å, Crystal Structure of Cas-DNA-PAM complex
5VVK 2.9 Å, Cas1-Cas2 bound to full-site mimic
5DS5 2.95 Å, Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA and Mg
5DQT 3.1 Å, Crystal Structure of Cas-DNA-22 complex
5DS4 3.2 Å, Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA
5VVL 3.31 Å, Cas1-Cas2 bound to full-site mimic with Ni
5DS6 3.35 Å, Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA with…
5WFE 3.64 Å, Cas1-Cas2-IHF-DNA holo-complex
5VVJ 3.89 Å, Cas1-Cas2 bound to half-site intermediate

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