Crystal structure of porcine aminopeptidase N complexed with CNGRCG tumor-homing peptide. Determined by X-ray diffraction at 1.95 Å resolution. Released 5 Nov 2014.
Explore 4OU3 in 3D Show helices and sheets RCSB PDB PDBe
4OU3 contains 48 α-helices and 38 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 66-68 | 3 | |
| α-helix | 72-73 | 2 | |
| β-strand | 76-87 | 12 | 1 |
| β-strand | 98-109 | 12 | 1 |
| β-strand | 113-119 | 7 | 2 |
| β-strand | 124-125 | 2 | 1 |
| β-strand | 133-136 | 4 | 1 |
| α-helix | 143-144 | 2 | |
| β-strand | 145-151 | 7 | 2 |
| β-strand | 156-165 | 10 | 2 |
| β-strand | 170-180 | 11 | 1 |
| β-strand | 187-195 | 9 | 3 |
| β-strand | 198-206 | 9 | 3 |
| α-helix | 212-214 | 3 | |
| β-strand | 218 | 1 | 2 |
| β-strand | 226 | 1 | 4 |
| β-strand | 227-235 | 9 | 1 |
| β-strand | 239-242 | 4 | 3 |
| β-strand | 246 | 1 | 5 |
| β-strand | 251-252 | 2 | 1 |
| β-strand | 259-263 | 5 | 1 |
| β-strand | 264 | 1 | 5 |
| α-helix | 265-268 | 4 | |
| β-strand | 270 | 1 | 4 |
| α-helix | 272-274 | 3 | |
| β-strand | 277-280 | 4 | 3 |
| β-strand | 283-288 | 6 | 6 |
| β-strand | 294-299 | 6 | 6 |
| α-helix | 301-305 | 5 | |
| α-helix | 310-326 | 17 | |
| β-strand | 335-341 | 7 | 6 |
| β-strand | 348-349 | 2 | 6 |
| β-strand | 354-358 | 5 | 6 |
| α-helix | 359-362 | 4 | |
| α-helix | 371-386 | 16 | |
| β-strand | 390 | 1 | 7 |
| β-strand | 394-396 | 3 | 7 |
| α-helix | 399-401 | 3 | |
| α-helix | 402-420 | 19 | |
| α-helix | 426-429 | 4 | |
| α-helix | 430 | 1 | |
| α-helix | 431-435 | 5 | |
| α-helix | 436-442 | 7 | |
| α-helix | 454-456 | 3 | |
| α-helix | 460-464 | 5 | |
| α-helix | 469 | 1 | |
| α-helix | 470-474 | 5 | |
| α-helix | 475-486 | 12 | |
| α-helix | 488-502 | 15 | |
| β-strand | 505-507 | 3 | 7 |
| α-helix | 509-521 | 13 | |
| α-helix | 532-540 | 9 | |
| β-strand | 546-551 | 6 | 8 |
| β-strand | 556-561 | 6 | 8 |
| α-helix | 568-570 | 3 | |
| β-strand | 582-584 | 3 | 9 |
| β-strand | 586-588 | 3 | 10 |
| β-strand | 591-592 | 2 | 10 |
| β-strand | 596-598 | 3 | 9 |
| β-strand | 603-605 | 3 | 8 |
| α-helix | 607-609 | 3 | |
| β-strand | 617-619 | 3 | 10 |
| α-helix | 621-623 | 3 | |
| β-strand | 628-631 | 4 | 8 |
| α-helix | 633-645 | 13 | |
| α-helix | 647-649 | 3 | |
| α-helix | 652-667 | 16 | |
| α-helix | 673-678 | 6 | |
| α-helix | 681-685 | 5 | |
| α-helix | 689-706 | 18 | |
| α-helix | 712-733 | 22 | |
| α-helix | 744-759 | 16 | |
| α-helix | 763-777 | 15 | |
| α-helix | 790-800 | 11 | |
| α-helix | 803-815 | 13 | |
| α-helix | 819-829 | 11 | |
| α-helix | 835-844 | 10 | |
| α-helix | 853-855 | 3 | |
| α-helix | 856-865 | 10 | |
| α-helix | 869-880 | 12 | |
| α-helix | 881-883 | 3 | |
| α-helix | 895-903 | 9 | |
| α-helix | 909-919 | 11 | |
| α-helix | 928-930 | 3 | |
| α-helix | 935-962 | 28 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 2 | 1 | 6 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Aminopeptidase N | A | protein | 908 | Sus scrofa | P15145 (AlphaFold model) |
| tumor-homing peptide | B | protein | 6 |
>4OU3_1 Aminopeptidase N (chains A) QSKPWNRYRLPTTLLPDSYNVTLRPYLTPNADGLYIFKGKSIVRFLCQEPTDVIIIHSKK LNYTTQGHMVVLRGVGDSQVPEIDRTELVELTEYLVVHLKGSLQPGHMYEMESEFQGELA DDLAGFYRSEYMEGNVKKVLATTQMQSTDARKSFPCFDEPAMKATFNITLIHPNNLTALS NMPPKGSSTPLAEDPNWSVTEFETTPVMSTYLLAYIVSEFQSVNETAQNGVLIRIWARPN AIAEGHGMYALNVTGPILNFFANHYNTSYPLPKSDQIALPDFNAGAMENWGLVTYRENAL LFDPQSSSISNKERVVTVIAHELAHQWFGNLVTLAWWNDLWLNEGFASYVEYLGADHAEP TWNLKDLIVPGDVYRVMAVDALASSHPLTTPAEEVNTPAQISEMFDSISYSKGASVIRML SNFLTEDLFKEGLASYLHAFAYQNTTYLDLWEHLQKAVDAQTSIRLPDTVRAIMDRWTLQ MGFPVITVDTKTGNISQKHFLLDSESNVTRSSAFDYLWIVPISSIKNGVMQDHYWLRDVS QAQNDLFKTASDDWVLLNVNVTGYFQVNYDEDNWRMIQHQLQTNLSVIPVINRAQVIYDS FNLATAHMVPVTLALDNTLFLNGEKEYMPWQAALSSLSYFSLMFDRSEVYGPMKKYLRKQ VEPLFQHFETLTKNWTERPENLMDQYSEINAISTACSNGLPQCENLAKTLFDQWMSDPEN NPIHPNLRSTIYCNAIAQGGQDQWDFAWGQLQQAQLVNEADKLRSALACSNEVWLLNRYL GYTLNPDLIRKQDATSTINSIASNVIGQPLAWDFVQSNWKKLFQDYGGGSFSFSNLIQGV TRRFSSEFELQQLEQFKKNNMDVGFGSGTRALEQALEKTKANIKWVKENKEVVLNWFIEH SSHHHHHH
>4OU3_2 tumor-homing peptide (chains B) CNGRCG
Water and common crystallization additives (SO4) are not listed.
A Unified Mechanism for Aminopeptidase N-based Tumor Cell Motility and Tumor-homing Therapy. Liu, C., Yang, Y., Chen, L. et al. J Biol Chem (2014) 289:34520-34529. DOI 10.1074/jbc.M114.566802 · PubMed
Other PDB entries of the same protein (UniProt P15145 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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