4R8G: Myosin-1c tail

Crystal Structure of Myosin-1c tail in complex with Calmodulin. Determined by X-ray diffraction at 3.5 Å resolution. Released 3 Dec 2014.

Method: X-ray diffraction
Resolution: 3.5 Å
Organisms: Mus musculus, Xenopus laevis
Chains: 4
Atoms: 5,570
Mol. weight: 88.56 kDa
Released: 3 Dec 2014

Explore 4R8G in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4R8G contains 37 α-helices and 18 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	6-19	14
β-strand	26-28	3	4
α-helix	29-38	10
α-helix	45-55	11
β-strand	62-64	3	4
α-helix	65-76	12
α-helix	82-90	9
α-helix	102-111	10
α-helix	118-127	10
α-helix	138-145	8

Chain B: 9 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	6-17	12
α-helix	29-31	3
α-helix	32-37	6
α-helix	45-55	11
α-helix	65-75	11
α-helix	83-90	8
β-strand	99-100	2	3
α-helix	102-111	10
α-helix	118-127	10
β-strand	136-137	2	3
α-helix	138-145	8

Chain E: 10 helices, 12 β-strands

Element	Residues	Length	Sheet
α-helix	699-760	62
α-helix	765	1
α-helix	768-786	19
α-helix	803-805	3
α-helix	806-827	22
α-helix	830-846	17
α-helix	853-856	4
α-helix	873-879	7
β-strand	886-893	8	1
β-strand	900-907	8	1
β-strand	911-915	5	1
β-strand	922-925	4	1
β-strand	929-935	7	1
β-strand	941-946	6	1
β-strand	957-961	5	1
α-helix	964-974	11
α-helix	978-980	3
β-strand	981-984	4	1
β-strand	987-990	4	2
β-strand	998-1004	7	2
β-strand	1009-1013	5	2
β-strand	1017-1022	6	2

Chain H: 10 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	6-21	16
α-helix	26-28	3
α-helix	29-38	10
α-helix	45-53	9
α-helix	66-74	9
α-helix	81-91	11
β-strand	99-100	2	5
α-helix	102-107	6
α-helix	108-112	5
α-helix	120-128	9
β-strand	136-137	2	5
α-helix	138-145	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Unconventional myosin-Ic	E	protein	335	Mus musculus	Q9WTI7 (AlphaFold model)
Calmodulin	A, B, H	protein	148	Xenopus laevis	P0DP33 (AlphaFold model)

Sequence of entity 1 (E), FASTA

>4R8G_1 Unconventional myosin-Ic (chains E)
GPGSRRQSLATKIQAAWRGFHWRQKFLRVKRSAICIQSWWRGTLGRRKAAKRKWAAQTIR
RLIRGFILRHSPRCPENAFFLDHVRASFLLNLRRQLPRNVLDTSWPTPPPALREASELLR
ELCMKNMVWKYCRSISPEWKQQLQQKAVASEIFKGKKDNYPQSVPRLFISTRLGTEEISP
RVLQSLGSEPIQYAVPVVKYDRKGYKPRPRQLLLTPSAVVIVEDAKVKQRIDYANLTGIS
VSSLSDSLFVLHVQREDNKQKGDVVLQSDHVIETLTKTALSADRVNNININQGSITFAGG
PGRDGIIDFTSGSELLITKAKNGHLAVVAPRLNSR

Sequence of entity 2 (A, B, H), FASTA

>4R8G_2 Calmodulin (chains A, B, H)
ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEE
VDEMIREADIDGDGQVNYEEFVQMMTAK

Primary citation

Structure of myosin-1c tail bound to calmodulin provides insights into calcium-mediated conformational coupling. Lu, Q., Li, J., Ye, F. et al. Nat Struct Mol Biol (2015) 22:81-88. DOI 10.1038/nsmb.2923 · PubMed

Other PDB entries of the same protein (UniProt Q9WTI7 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9CFV 2.7 Å, Cryo-EM structure of delta-NTR myosin-1c bound to F-actin
9CFX 2.7 Å, Cryo-EM structure of myosin-1c bound to F-actin in the Rigor state
9CFU 2.8 Å, Cryo-EM structure of myosin-1c bound to F-actin in the ADP-A state
9CFW 3.0 Å, Cryo-EM structure of myosin-1c bound to F-actin in the ADP-B state

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