4UAF: Importin alpha 1 delta IBB

Importin alpha 1 delta IBB in complex with Influenza PB2 nuclear localization domain. Determined by X-ray diffraction at 1.7 Å resolution. Released 4 Feb 2015.

Method: X-ray diffraction
Resolution: 1.7 Å
Organisms: Mus musculus, Influenza A virus
Chains: 2
Atoms: 4,288
Mol. weight: 59.99 kDa
Ligands: PO4
Released: 4 Feb 2015

Explore 4UAF in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4UAF contains 40 α-helices and 4 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 33 helices, 0 β-strands

Element	Residues	Length
α-helix	78-85	8
α-helix	90-105	16
α-helix	112-117	6
α-helix	121-128	8
α-helix	134-148	15
α-helix	152-160	9
α-helix	163-170	8
α-helix	176-191	16
α-helix	194-202	9
α-helix	206-211	6
α-helix	218-220	3
α-helix	223-236	14
α-helix	243-245	3
α-helix	246-259	14
α-helix	265-278	14
α-helix	283-290	8
α-helix	295-302	8
α-helix	307-320	14
α-helix	325-333	9
α-helix	336-339	4
α-helix	340-343	4
α-helix	349-362	14
α-helix	367-375	9
α-helix	378-387	10
α-helix	391-407	17
α-helix	410-418	9
α-helix	422-427	6
α-helix	428-430	3
α-helix	434-454	21
α-helix	457-466	10
α-helix	469-475	7
α-helix	476-478	3
α-helix	482-495	14

Chain E: 7 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	688-691	4
β-strand	694-699	6	1
α-helix	702-704	3
α-helix	707	1
β-strand	708	1	1
α-helix	709	1
α-helix	710-713	4
α-helix	716-717	2
β-strand	721-726	6	1
β-strand	730-736	7	1
α-helix	749-750	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Importin subunit alpha-1	B	protein	466	Mus musculus	P52293 (AlphaFold model)
Polymerase basic protein 2	E	protein	87	Influenza A virus	P31345

Sequence of entity 1 (B), FASTA

>4UAF_1 Importin subunit alpha-1 (chains B)
MADIGSNQGTVNWSVEDIVKGINSNNLESQLQATQAARKLLSREKQPPIDNIIRAGLIPK
FVSFLGKTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAV
WALGNIAGDGSAFRDLVIKHGAIDPLLALLAVPDLSTLACGYLRNLTWTLSNLCRNKNPA
PPLDAVEQILPTLVRLLHHNDPEVLADSCWAISYLTDGPNERIEMVVKKGVVPQLVKLLG
ATELPIVTPALRAIGNIVTGTDEQTQKVIDAGALAVFPSLLTNPKTNIQKEATWTMSNIT
AGRQDQIQQVVNHGLVPFLVGVLSKADFKTQKEAAWAITNYTSGGTVEQIVYLVHCGIIE
PLMNLLSAKDTKIIQVILDAISNIFQAAEKLGETEKLSIMIEECGGLDKIEALQRHENES
VYKASLNLIEKYFSVEEEEDQNVVPETTSEGFAFQVQDGAPGTFNF

Sequence of entity 2 (E), FASTA

>4UAF_2 Polymerase basic protein 2 (chains E)
GPLGSDPDESTSGVESAVLRGFLILGKEDRRYGPALSINELSNLAKGEKANVLIGQGDVV
LVMKRKRDSSILTDSQTATKRIRMAIN

Ligands and cofactors

ID	Name	Formula	Copies
PO4	Phosphate ion	O4 P	2

Primary citation

Molecular Determinants for Nuclear Import of Influenza A PB2 by Importin alpha Isoforms 3 and 7. Pumroy, R.A., Ke, S., Hart, D.J. et al. Structure (2015) 23:374-384. DOI 10.1016/j.str.2014.11.015 · PubMed

Other PDB entries of the same protein (UniProt P52293 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5CTT 1.7 Å, Crystal structure of human SART3/TIP110 NLS-mouse importin alpha complex
6IUA 1.7 Å, Crystal structure of importin-alpha1 bound to the 53BP1 nuclear localization signal…
6K06 1.75 Å, Crystal structure of Importin-alpha and phosphomimetic GM130
8HE0 1.8 Å, Crystal structure of importin-alpha1 bound to the HIF-1alpha nuclear localization signal…
4YI0 1.81 Å, Structure of mouse importin a1 bound to Pom121NLS
7RG1 1.85 Å, Importin alpha2 in complex with MERS ORF4B H26A mutant
4MZ6 1.88 Å, Structure of importin-alpha: dUTPase S11E NLS mutant complex
3UL1 1.9 Å, Mouse importin alpha: nucleoplasmin cNLS peptide complex
5D5K 1.9 Å, Crystal Structure NLS from human PARP-2 complexed with Importin alpha delta IBB
5UMZ 1.9 Å, Structure of TNRC6A NLS in complex with importin-alpha
6IU7 1.9 Å, Crystal structure of importin-alpha1 bound to the 53BP1 nuclear localization signal…
8FUA 1.9 Å, Crystal structure of mouse Importin alpha in complex with Hendra virus matrix protein NLS1

Browse structure collections

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