4UX3: Cohesin Smc3-HD:Scc1-N complex from yeast

cohesin Smc3-HD:Scc1-N complex from yeast. Determined by X-ray diffraction at 3.3 Å resolution. Released 3 Dec 2014.

Method: X-ray diffraction
Resolution: 3.3 Å
Organism: SACCHAROMYCES CEREVISIAE
Chains: 2
Atoms: 4,098
Mol. weight: 76.11 kDa
Ligands: AGS, MG
Released: 3 Dec 2014

Explore 4UX3 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4UX3 contains 17 α-helices and 13 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 14 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	6-11	6	1
β-strand	14-17	4	1
β-strand	27-32	6	2
α-helix	38-49	12
α-helix	58-64	7
β-strand	77-80	4	1
β-strand	106	1	1
α-helix	125-133	9
β-strand	145-146	2	2
α-helix	152-154	3
α-helix	159-167	9
α-helix	172-237	66
α-helix	239-244	6
α-helix	976-980	5
α-helix	982-988	7
α-helix	998-1059	62
β-strand	1065-1069	5	3
β-strand	1109-1113	5	3
β-strand	1121-1122	2	3
α-helix	1128-1143	16
β-strand	1150-1153	4	2
α-helix	1162-1176	15
β-strand	1180-1184	5	2
α-helix	1188-1190	3
β-strand	1198-1203	6	2
β-strand	1206-1210	5	2
α-helix	1214-1221	8

Chain B: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	26-28	3
α-helix	44-51	8
α-helix	69-100	32

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Structural maintenance of chromosomes protein 3	A	protein	543	SACCHAROMYCES CEREVISIAE	P47037 (AlphaFold model)
Mitotic chromosome determinant-related protein	B	protein	121	SACCHAROMYCES CEREVISIAE	Q12158 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>4UX3_1 STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3 (chains A)
MAYIKRVIIKGFKTYRNETIIDNFSPHQNVIIGSNGSGKSNFFAAIRFVLSDDYSNLKRE
ERQGLIHQGSGGSVMSASVEIVFHDPDHSMILPSGVLSRGDDEVTIRRTVGLKKDDYQLN
DRNVTKGDIVRMLETAGFSMNNPYNIVPQGKIVALTNAKDKERLQLLEDVVGAKSFEVKL
KASLKKMEETEQKKIQINKEMGELNSKLSEMEQERKELEKYNELERNRKIYQFTLYDREL
NEVINQMERLDGDYNNTVYSSESSKHPTSLVPRGSDITSDQLLQRLNDMNTEISGLKNVN
KRAFENFKKFNERRKDLAERASELDESKDSIQDLIVKLKQQKVNAVDSTFQKVSENFEAV
FERLVPRGTAKLIIHRKNDNANDHDESIDVDMDAESNESQNGKDSEIMYTGVSISVSFNS
KQNEQLHVEQLSGGQKTVCAIALILAIQMVDPASFYLFDEIDAALDKQYRTAVATLLKEL
SKNAQFICTTFRTDMLQVADKFFRVKYENKISTVIEVNREEAIGFIRGSNKFAEVWSHPQ
FEK

Sequence of entity 2 (B), FASTA

>4UX3_2 MITOTIC CHROMOSOME DETERMINANT-RELATED PROTEIN (chains B)
MVTENPQRLTVLRLATNKGPLAQIWLASNMSNIPRGSVIQTHIAESAKEIAKASGCDDES
GDNEYITLRTSGELLQGIVRVYSKQATFLLTDIKDTLTKISMLFKTSQKMTSTVNHHHHH
H

Ligands and cofactors

ID	Name	Formula	Copies
AGS	Phosphothiophosphoric acid-adenylate ester	C10 H16 N5 O12 P3 S	1
MG	Magnesium ion	Mg	1

Primary citation

Closing the Cohesin Ring: Structure and Function of its Smc3-Kleisin Interface. Gligoris, T.G., Scheinost, J.C., Burmann, F. et al. Science (2014) 346:963. DOI 10.1126/SCIENCE.1256917 · PubMed

Other PDB entries of the same protein (UniProt P47037 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6QPW 3.3 Å, Structural basis of cohesin ring opening
6ZZ6 3.4 Å, Cryo-EM structure of S.cerevisiae cohesin-Scc2-DNA complex
7OGT 5.5 Å, Folded elbow of cohesin

Browse structure collections

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