4V9L: 30S ribosomal protein S2

70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Determined by X-ray diffraction at 3.5 Å resolution. Released 9 Jul 2014.

Method: X-ray diffraction
Resolution: 3.5 Å
Organisms: Thermus thermophilus, Escherichia coli, Streptomyces
Chains: 122
Atoms: 308,166
Mol. weight: 4570.58 kDa
Ligands: FUA, GDP, MG
Released: 9 Jul 2014

Explore 4V9L in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4V9L contains 445 α-helices and 724 β-strands across 105 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain AB: 12 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	8-15	8
α-helix	27-31	5
β-strand	32-33	2	1
α-helix	36-38	3
β-strand	41-42	2	1
α-helix	44-64	21
β-strand	71	1	2
α-helix	75-88	14
β-strand	93	1	2
α-helix	105-122	18
α-helix	132-148	17
α-helix	152-154	3
β-strand	162	1	3
α-helix	169-171	3
α-helix	172-179	8
β-strand	184	1	3
β-strand	185-188	4	4
β-strand	199-202	4	4
α-helix	208-224	17
α-helix	236-240	5

Chain AC: 7 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	7-10	4
β-strand	21-22	2	16
α-helix	26-46	21
α-helix	48-50	3
β-strand	52	1	17
β-strand	57	1	17
β-strand	58-59	2	16
β-strand	63	1	18
β-strand	66-70	5	17
α-helix	74-76	3
α-helix	83-94	12
β-strand	98	1	18
β-strand	101-105	5	17
α-helix	113-125	13
α-helix	130-144	15
β-strand	148-154	7	19
β-strand	165-166	2	19
β-strand	169-170	2	19
β-strand	182-191	10	19
β-strand	194-203	10	19

Chain AD: 10 helices, 10 β-strands

Element	Residues	Length	Sheet
α-helix	10-15	6
β-strand	26	1	32
β-strand	28	1	32
β-strand	30	1	32
α-helix	53-67	15
α-helix	72-84	13
α-helix	89-98	10
β-strand	100	1	33
α-helix	101-108	8
α-helix	114-122	9
β-strand	126-128	3	34
β-strand	131-132	2	34
β-strand	139	1	33
β-strand	144-148	5	34
α-helix	151-154	4
α-helix	156-165	10
α-helix	169-171	3
β-strand	174-177	4	34
β-strand	182-185	4	34
α-helix	200-207	8

Chain AE: 4 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	8-15	8	39
β-strand	18	1	40
β-strand	25	1	40
β-strand	28-34	7	39
β-strand	40	1	41
β-strand	42-48	7	39
α-helix	51-64	14
β-strand	68	1	41
β-strand	72	1	42
β-strand	75	1	42
β-strand	80-82	3	43
β-strand	87-93	7	43
β-strand	99	1	44
α-helix	104-111	8
β-strand	117	1	44
β-strand	118-124	7	43
α-helix	128-140	13
α-helix	145-151	7

Chain AF: 2 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	2-10	9	53
α-helix	16-32	17
β-strand	41-52	12	53
β-strand	55-67	13	53
α-helix	71-79	9
β-strand	87-92	6	53
β-strand	98	1	54

Chain AG: 7 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	21-30	10
β-strand	32	1	56
β-strand	35	1	56
α-helix	36-53	18
α-helix	58-68	11
β-strand	73-75	3	57
β-strand	80	1	58
β-strand	83	1	58
β-strand	88-90	3	57
α-helix	93-110	18
α-helix	116-129	14
α-helix	133-147	15
α-helix	149-151	3

Chain AH: 5 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	5-19	15
β-strand	23-27	5	80
α-helix	30-40	11
β-strand	45-53	9	80
β-strand	56-63	8	80
α-helix	76-78	3
β-strand	83-85	3	81
α-helix	104-106	3
β-strand	109-114	6	81
β-strand	118-119	2	81
α-helix	121-127	7
β-strand	131-137	7	81

Chain AI: 7 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	6-9	4	89
β-strand	14-20	7	89
β-strand	25-28	4	89
β-strand	31-32	2	89
α-helix	33-36	4
α-helix	42-45	4
α-helix	48-51	4
β-strand	60-66	7	89
α-helix	70-84	15
α-helix	90-96	7
α-helix	97-99	3
α-helix	122-125	4

94 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
30S ribosomal protein S2	AB, CB	protein	235	Thermus thermophilus	P62662 (AlphaFold model)
30S ribosomal protein S3	AC, CC	protein	207	Thermus thermophilus	P62663 (AlphaFold model)
30S ribosomal protein S4	AD, CD	protein	208	Thermus thermophilus	P62664 (AlphaFold model)
30S ribosomal protein S5	AE, CE	protein	151	Thermus thermophilus	P62665 (AlphaFold model)
30S ribosomal protein S6	AF, CF	protein	101	Thermus thermophilus	P62666
30S ribosomal protein S7	AG, CG	protein	155	Thermus thermophilus	P62667
30S ribosomal protein S8	AH, CH	protein	138	Thermus thermophilus	P62668
30S ribosomal protein S9	AI, CI	protein	127	Thermus thermophilus	P62669
30S ribosomal protein S10	AJ, CJ	protein	99	Thermus thermophilus	P62653
30S ribosomal protein S11	AK, CK	protein	119	Thermus thermophilus	P62654
30S ribosomal protein S12	AL, CL	protein	125	Thermus thermophilus	P61941
30S ribosomal protein S13	AM, CM	protein	125	Thermus thermophilus	P62655

48 more molecules are not listed.

Sequence of entity 1 (AB, CB), FASTA

>4V9L_1 30S ribosomal protein S2 (chains AB, CB)
VKELLEAGVHFGHERKRWNPKFARYIYAERNGIHIIDLQKTMEELERTFRFIEDLAMRGG
TILFVGTKKQAQDIVRMEAERAGMPYVNQRWLGGMLTNFKTISQRVHRLEELEALFASPE
IEERPKKEQVRLKHELERLQKYLSGFRLLKRLPDAIFVVDPTKEAIAVREARKLFIPVIA
LADTDSDPDLVDYIIPGNDDAIRSIQLILSRAVDLIIQARGGVVEPSPSYALVQE

Sequence of entity 2 (AC, CC), FASTA

>4V9L_2 30S ribosomal protein S3 (chains AC, CC)
GNKIHPIGFRLGITRDWESRWYAGKKQYRHLLLEDQRIRGLLEKELYSAGLARVDIERAA
DNVAVTVHVAKPGVVIGRGGERIRVLREELAKLTGKNVALNVQEVQNPNLSAPLVAQRVA
EQIERRFAVRRAIKQAVQRVMESGAKGAKVIVSGRIGGAEQARTEWAAQGRVPLHTLRAN
IDYGFALARTTYGVLGVKAYIFLGEVI

Sequence of entity 3 (AD, CD), FASTA

>4V9L_3 30S ribosomal protein S4 (chains AD, CD)
GRYIGPVCRLCRREGVKLYLKGERCYSPKCAMERRPYPPGQHGQKRARRPSDYAVRLREK
QKLRRIYGISERQFRNLFEEASKKKGVTGSVFLGLLESRLDNVVYRLGFAVSRRQARQLV
RHGHITVNGRRVDLPSYRVRPGDEIAVAEKSRNLELIRQNLEAMKGRKVGPWLSLDVEGM
KGKFLRLPDREDLALPVNEQLVIEFYSR

Sequence of entity 4 (AE, CE), FASTA

>4V9L_4 30S ribosomal protein S5 (chains AE, CE)
DFEEKMILIRRTARMQAGGRRFRFGALVVVGDRQGRVGLGFGKAPEVPLAVQKAGYYARR
NMVEVPLQNGTIPHEIEVEFGASKIVLKPAAPGTGVIAGAVPRAILELAGVTDILTKELG
SRNPINIAYATMEALRQLRTKADVERLRKGE

Sequence of entity 5 (AF, CF), FASTA

>4V9L_5 30S ribosomal protein S6 (chains AF, CF)
MRRYEVNIVLNPNLDQSQLALEKEIIQRALENYGARVEKVEELGLRRLAYPIAKDPQGYF
LWYQVEMPEDRVNDLARELRIRDNVRRVMVVKSQEPFLANA

Sequence of entity 6 (AG, CG), FASTA

>4V9L_6 30S ribosomal protein S7 (chains AG, CG)
ARRRRAEVRQLQPDLVYGDVLVTAFINKIMRDGKKNLAARIFYDACKIIQEKTGQEPLKV
FKQAVENVKPRMEVRSRRVGGANYQVPMEVSPRRQQSLALRWLVQAANQRPERRAAVRIA
HELMDAAEGKGGAVKKKEDVERMAEANRAYAHYRW

Sequence of entity 7 (AH, CH), FASTA

>4V9L_7 30S ribosomal protein S8 (chains AH, CH)
MLTDPIADMLTRIRNATRVYKESTDVPASRFKEEILRILAREGFIKGYERVDVDGKPYLR
VYLKYGPRRQGPDPRPEQVIHHIRRISKPGRRVYVGVKEIPRVRRGLGIAILSTSKGVLT
DREARKLGVGGELICEVW

Sequence of entity 8 (AI, CI), FASTA

>4V9L_8 30S ribosomal protein S9 (chains AI, CI)
EQYYGTGRRKEAVARVFLRPGNGKVTVNGQDFNEYFQGLVRAVAALEPLRAVDALGHFDA
YITVRGGGKSGQIDAIKLGIARALVQYNPDYRAKLKPLGFLTRDARVVERKKYGKHKARR
APQYSKR

Sequence of entity 9 (AJ, CJ), FASTA

>4V9L_9 30S ribosomal protein S10 (chains AJ, CJ)
KIRIKLRGFDHKTLDASAQKIVEAARRSGAQVSGPIPLPTRVRRFTVIRGPFKHKDSREH
FELRTHNRLVDIINPNRKTIEQLMTLDLPTGVEIEIKTV

Sequence of entity 10 (AK, CK), FASTA

>4V9L_10 30S ribosomal protein S11 (chains AK, CK)
KRQVASGRAYIHASYNNTIVTITDPDGNPITWSSGGVIGYKGSRKGTPYAAQLAALDAAK
KAMAYGMQSVDVIVRGTGAGREQAIRALQASGLQVKSIVDDTPVPHNGCRPKKKFRKAS

Sequence of entity 11 (AL, CL), FASTA

>4V9L_11 30S ribosomal protein S12 (chains AL, CL)
PTINQLVRKGREKVRKKSKVPALKGAPFRRGVCTVVRTVTPKKPNSALRKVAKVRLTSGY
EVTAYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGVYDAAGVKDRKKSRSKYGTKK
PKEAA

Sequence of entity 12 (AM, CM), FASTA

>4V9L_12 30S ribosomal protein S13 (chains AM, CM)
ARIAGVEIPRNKRVDVALTYIYGIGKARAKEALEKTGINPATRVKDLTEAEVVRLREYVE
NTWKLEGELRAEVAANIKRLMDIGCYRGLRHRRGLPVRGQRTRTNARTRKGPRKTVAGKK
KAPRK

Ligands and cofactors

ID	Name	Formula	Copies
FUA	Fusidic acid	C31 H48 O6	2
GDP	Guanosine-5'-diphosphate	C10 H15 N5 O11 P2	2
MG	Magnesium ion	Mg	2

Primary citation

Crystal structures of EF-G-ribosome complexes trapped in intermediate states of translocation. Zhou, J., Lancaster, L., Donohue, J.P. et al. Science (2013) 340:1236086-1236086. DOI 10.1126/science.1236086 · PubMed

Other PDB entries of the same protein (UniProt P62662 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5J4D 3.1 Å, E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated…
6N1D 3.2 Å, X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into…
4V63 3.21 Å, Structural basis for translation termination on the 70S ribosome.
5V8I 3.25 Å, Thermus thermophilus 70S ribosome lacking ribosomal protein uS17
4V84 3.4 Å, Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S…
4V9N 3.4 Å, Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2.
4V9Q 3.4 Å, Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome.
6B4V 3.4 Å, Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome
6BOH 3.4 Å, Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304)…
4V83 3.5 Å, Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to…
4V9K 3.5 Å, 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP,…
6BOK 3.55 Å, E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome

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