5A5U: Eukaryotic initiation factor 3

Structure of mammalian eIF3 in the context of the 43S preinitiation complex. Determined by electron microscopy at 9.0 Å resolution. Released 9 Sept 2015.

Method: Electron microscopy
Resolution: 9.0 Å
Organism: ORYCTOLAGUS CUNICULUS
Chains: 3
Atoms: 8,124
Mol. weight: 167.82 kDa
Released: 9 Sept 2015

Explore 5A5U in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5A5U contains 17 α-helices and 78 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	664-712	49

Chain B: 11 helices, 48 β-strands

Element	Residues	Length	Sheet
β-strand	89-93	5	1
α-helix	102-113	12
β-strand	116	1	2
β-strand	118	1	2
β-strand	120-124	5	1
β-strand	128	1	3
β-strand	131	1	3
α-helix	132-133	2
β-strand	135-140	6	1
α-helix	145-152	8
β-strand	157	1	4
β-strand	163	1	4
β-strand	165-167	3	1
α-helix	190-201	12
β-strand	207-213	7	5
β-strand	218-224	7	5
α-helix	229-230	2
β-strand	231-236	6	5
β-strand	243-245	3	6
β-strand	252-254	3	6
β-strand	260-264	5	6
β-strand	269-274	6	6
β-strand	281-284	4	7
β-strand	290-294	5	7
β-strand	307-311	5	7
β-strand	316-321	6	7
α-helix	330	1
β-strand	333-334	2	8
β-strand	340-345	6	8
β-strand	348-353	6	8
β-strand	358-365	8	8
β-strand	372-375	4	9
β-strand	381-386	6	9
β-strand	389	1	10
β-strand	392	1	10
β-strand	395-400	6	9
β-strand	405-411	7	9
β-strand	415-422	8	11
β-strand	428-435	8	11
β-strand	444-451	8	11
β-strand	459-465	7	11
β-strand	468-473	6	12
α-helix	474	1
β-strand	480-484	5	12
β-strand	490-497	8	12
β-strand	503-512	10	12
β-strand	516-519	4	13
β-strand	525-529	5	13
β-strand	537-542	6	13
β-strand	547-553	7	13
β-strand	559-562	4	14
β-strand	568-572	5	14
β-strand	583-588	6	14
β-strand	592-598	7	14
β-strand	604-607	4	5
α-helix	608-609	2
α-helix	617-622	6
α-helix	635-644	10
α-helix	650-675	26

Chain I: 5 helices, 30 β-strands

Element	Residues	Length	Sheet
β-strand	2-6	5	15
β-strand	13-18	6	16
β-strand	24-29	6	16
β-strand	34-38	5	16
β-strand	44-48	5	16
β-strand	55-60	6	17
β-strand	66-71	6	17
β-strand	75-80	6	17
β-strand	85-91	7	17
β-strand	96-101	6	18
β-strand	107-112	6	18
α-helix	113	1
β-strand	121-126	6	18
β-strand	127-129	3	19
β-strand	136-139	4	19
β-strand	145-148	4	18
β-strand	156-161	6	20
α-helix	163-165	3
β-strand	167-172	6	20
β-strand	176-181	6	20
β-strand	187-193	7	20
β-strand	199-204	6	21
β-strand	210-215	6	21
β-strand	219-224	6	21
β-strand	230-235	6	21
β-strand	240-245	6	22
α-helix	246	1
β-strand	251-256	6	22
β-strand	273-279	7	22
β-strand	285-292	8	22
β-strand	296-301	6	15
β-strand	307-312	6	15
β-strand	317-321	5	15
α-helix	324-327	4
α-helix	332-339	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Eukaryotic initiation factor 3	A	protein	54	ORYCTOLAGUS CUNICULUS
Eukaryotic translation initiation factor 3 subunit B	B	protein	1121	ORYCTOLAGUS CUNICULUS	G1SZ03 (AlphaFold model)
Eukaryotic translation initiation factor 3 subunit I	I	protein	347	ORYCTOLAGUS CUNICULUS	P40217 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>5A5U_1 EUKARYOTIC INITIATION FACTOR 3 (chains A)
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Sequence of entity 2 (B), FASTA

>5A5U_2 EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT B (chains B)
MLGAVKMEGHESSDWNSYYADTQEAYSSVPVSNMNSGLGSMNSMNTYMTMNTMTTSGNMT
PASFNMSYANPGLGAGLSPGAVAGMPAGSAGAMNSMTAAGVTAMGTALSPGGMGAMGAQP
AASMNGLGPYAAAMNPCMSPMAYAPSNLGRSRAGGGGDAKTFKRSYPHAKPPYSYISLIT
MAIQQAPSKMLTLSEIYQWIMDLFPYYRQNQQRWQNSIRHSLSFNDCFLKVPRSPDKPGK
GSFWTLHPDSGNMFENGCYLRRQKRFKCEKQLALKEAGAPGAGAGGKKAAAVGAQASQAQ
LGEVAGPAPDTPAGTESPHSSASPCQEHKRVALGELKGSAAGAALSPPEPAPSPGQQQQQ
QPGAEPPPEEEPPRPAGAEDAAAEAAAEAGPEPDVRAEPAEAQEAASSPAEPPATLPAEQ
GEAPREQARDEPAPASEAEGSAAEAEPRAVENGDADEPSFSDPEDFVDDVSEEELLGDVL
KDRPQEADGIDSVIVVDNVPQVGPDRLEKLKNVIHKIFSKFGKITNDFYPEEDGKTKGYI
FLEYASPAHAVDAVKNADGYKLDKQHTFRVNLFTDFDKYMTISDEWDIPEKQPFKDLGNL
RYWLEEAECRDQYSVIFESGDRTSIFWNDVKDPVSIEERARWTETYVRWSPKGTYLATFH
QRGIALWGGEKFKQIQRFSHQGVQLIDFSPCERYLVTFSPLMDTQDDPQAIIIWDILTGH
KKRGFHCESSAHWPIFKWSHDGKFFARMTLDTLSIYETPSMGLLDKKSLKISGIKDFSWS
PGGNIIAFWVPEDKDIPARVTLMQLPSRQEIRVRNLFNVVDCKLHWQKNGDYLCVKVDRT
PKGTQGVVTNFEIFRMREKQVPVDVVEMKETIIAFAWEPNGSKFAVLHGEAPRISVSFYH
VKSNGKIELSKMFDKQQANTIFWSPQGQFVVLAGLRSMNGALAFVDTSDCTVMNIAEHYM
ASDVEWDPTGRYVVTSVSWWSHKVDNAYWLWTFQGRLLQKNNKDRFCQLLWRPRPPTLLS
QEQIKQIKKDLKKYSKIFEQKDRLSQSKASKELVERRRAMMEDFRKYGKAAQELYVGQKN
ERLELRGGVDTDELDSNVDDWEEETIEFFVTEEVIPLGSQE

Sequence of entity 3 (I), FASTA

>5A5U_3 EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I (chains I)
MKAIKLTGHERPLTQVKYNKEGDLLFSCSKDSSASVWYSLNGERLGTLDGHTGTIWSIDV
DCFTKYCVTGSADYSIKLWDVSNGQCVATWKSPVPVKRVEFSPCGNYFLAILDNVMKNPG
SINIYEIERDSATHELTKVSEEPIHKIITHEGLDAATVAGWSTKGKYIIAGHKDGKISKY
DVSNNYEYVDSIDLHEKSISDMQFSPDLTYFITSSRDTNSFLVDVSTLQVLKKYETDCPL
NTAVITPLKEFIILGGGQEAKDVTTTSANEGKFEARFYHKIFEEEIGRVQGHFGPLNTVA
ISPQGTSYASGGEDGFIRLHHFEKSYFDFKYDVEKAAEAKEHMQEAN

Primary citation

Structure of Mammalian Eif3 in the Context of the 43S Preinitiation Complex. Des-Georges, A., Dhote, V., Kuhn, L. et al. Nature (2015) 525:491. DOI 10.1038/NATURE14891 · PubMed

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