5AFP: Neuronal calcium sensor 1

Neuronal calcium sensor-1 (NCS-1)from Rattus norvegicus complex with rhodopsin kinase peptide from Homo sapiens. Determined by X-ray diffraction at 2.3 Å resolution. Released 18 Mar 2015.

Method: X-ray diffraction
Resolution: 2.3 Å
Organisms: RATTUS NORVEGICUS, HOMO SAPIENS
Chains: 4
Atoms: 3,022
Mol. weight: 49.19 kDa
Ligands: CA
Released: 18 Mar 2015

Explore 5AFP in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5AFP contains 24 α-helices and 8 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	10-18	9
α-helix	24-37	14
β-strand	42-43	2	1
α-helix	45-55	11
α-helix	62-72	11
β-strand	80-81	2	1
α-helix	82-94	13
α-helix	97-108	12
β-strand	116-117	2	2
α-helix	118-132	15
α-helix	140-142	3
α-helix	145-156	12
β-strand	163-164	2	2
α-helix	166-175	10
α-helix	177-182	6

Chain B: 11 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	10-19	10
α-helix	24-37	14
β-strand	42-43	2	3
α-helix	45-53	9
α-helix	62-72	11
β-strand	80-81	2	3
α-helix	82-94	13
α-helix	97-108	12
β-strand	116-117	2	4
α-helix	118-131	14
α-helix	140-142	3
α-helix	145-156	12
β-strand	163-164	2	4
α-helix	166-175	10
α-helix	177-182	6

Chains C and D: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	7-14	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Neuronal calcium sensor 1	A, B	protein	190	RATTUS NORVEGICUS	P62168 (AlphaFold model)
Rhodopsin kinase	C, D	protein	25	HOMO SAPIENS	Q15835 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>5AFP_1 NEURONAL CALCIUM SENSOR 1 (chains A, B)
MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGD
PTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNE
MLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIV
QALSLYDGLV

Sequence of entity 2 (C, D), FASTA

>5AFP_2 RHODOPSIN KINASE (chains C, D)
MDFGSLETVVANSAFIAARGSFDGS

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	6

Water and common crystallization additives (NA) are not listed.

Primary citation

Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and G-Protein Coupled Receptor Kinase 1 (Grk1) Peptides Using Different Modes of Interactions. Pandalaneni, S., Karuppiah, V., Saleem, M. et al. J Biol Chem (2015) 290:18744. DOI 10.1074/JBC.M114.627059 · PubMed

Other PDB entries of the same protein (UniProt P62168 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5AEQ 1.95 Å, Neuronal calcium sensor (NCS-1)from Rattus norvegicus
5AER 2.19 Å, Neuronal calcium sensor-1 (NCS-1)from Rattus norvegicus complex with D2 dopamine…
4OV2 2.6 Å, Crystal structure of C-terminally truncated Neuronal Calcium Sensor (NCS-1) from Rattus…
4YRU 2.8 Å, Crystal structure of C-terminally truncated Neuronal Calcium Sensor (NCS-1) from Rattus…

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