5AIE: Not4 ring domain

Not4 ring domain in complex with Ubc4. Determined by X-ray diffraction at 2.8 Å resolution. Released 29 Apr 2015.

Method: X-ray diffraction
Resolution: 2.8 Å
Organism: SACCHAROMYCES CEREVISIAE
Chains: 2
Atoms: 1,575
Mol. weight: 24.28 kDa
Ligands: ZN
Released: 29 Apr 2015

Explore 5AIE in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5AIE contains 9 α-helices and 12 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 3 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	32	1	1
β-strand	39	1	1
α-helix	58-66	9
β-strand	73	1	2
α-helix	79	1
β-strand	80	1	2
α-helix	81	1

Chain B: 6 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	2-16	15
β-strand	24-26	3	3
β-strand	33-39	7	3
α-helix	40-41	2
β-strand	50-56	7	3
β-strand	67-70	4	3
β-strand	76	1	4
β-strand	79	1	4
β-strand	84	1	3
β-strand	85	1	4
α-helix	100-112	13
α-helix	122-130	9
α-helix	132-142	11
α-helix	143-147	5

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
General negative regulator of transcription subunit 4	A	protein	57	SACCHAROMYCES CEREVISIAE	P34909 (AlphaFold model)
Ubiquitin-conjugating enzyme E2 4	B	protein	158	SACCHAROMYCES CEREVISIAE	P15731 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>5AIE_1 GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 4 (chains A)
RSMEDYCPLCIEPMDITDKNFFPCPCGYQICQFCYNNIRQNPELNGRCPACRRKYDD

Sequence of entity 2 (B), FASTA

>5AIE_2 UBIQUITIN-CONJUGATING ENZYME E2 4 (chains B)
TGSTGSTETGMSSSKRIAKELSDLERDPPTSCSAGPVGDDLYHWQASIMGPADSPYAGGV
FFLSIHFPTDYPFKPPKISFTTKIYHPNINANGNICLDILKDQWSPALTLSKVLLSICSL
LTDANPDDPLVPEIAHIYKTDRPKYEATAREWTKKYAV

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	2

Primary citation

Architecture of the Ubiquitylation Module of the Yeast Ccr4-not Complex. Bhaskar, V., Basquin, J., Conti, E. Structure (2015) 23:921. DOI 10.1016/J.STR.2015.03.011 · PubMed

Other PDB entries of the same protein (UniProt P34909 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5AJD 3.62 Å, Not1 C-terminal domain in complex with Not4

Browse structure collections

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