5FCI: Vacant uL3 W255C mutant 80S yeast ribosome

Name: PDB 5FCI: Structure of the vacant uL3 W255C mutant 80S yeast ribosome
Published: 2016-05-11
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: ribosome

Structure of the vacant uL3 W255C mutant 80S yeast ribosome. Determined by X-ray diffraction at 3.4 Å resolution. Released 11 May 2016.

Method: X-ray diffraction
Resolution: 3.4 Å
Organism: Saccharomyces cerevisiae S288c
Chains: 162
Atoms: 411,776
Mol. weight: 6586.42 kDa
Ligands: OHX, MG, ZN
Released: 11 May 2016

Explore 5FCI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5FCI contains 942 α-helices and 1,059 β-strands across 154 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain c0: 4 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	5-17	13
β-strand	20-21	2	180
α-helix	39-49	11
α-helix	50-52	3
β-strand	55-56	2	180
β-strand	59	1	181
β-strand	64	1	181
β-strand	66-68	3	180
α-helix	72-79	8

Chain C0: 4 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	5-18	14
β-strand	20-22	3	167
α-helix	39-51	13
β-strand	55-58	4	167
β-strand	65-68	4	167
α-helix	70-79	10
α-helix	82-83	2

Chain c1: 1 helix, 7 β-strands

Element	Residues	Length	Sheet
β-strand	39	1	188
β-strand	42	1	188
α-helix	46-49	4
β-strand	70-77	8	189
β-strand	84-94	11	189
β-strand	99-110	12	189
β-strand	122-132	11	189
β-strand	135-142	8	189

Chain C1: 3 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	13-14	2
α-helix	46-50	5
β-strand	70-80	11	175
β-strand	83-94	12	175
β-strand	99-110	12	175
β-strand	122-127	6	175
β-strand	137-141	5	175
α-helix	150-152	3

Chain c2: 6 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	34-37	4
β-strand	41-43	3	202
α-helix	46-52	7
β-strand	60-62	3	202
α-helix	70-79	10
α-helix	95-99	5
β-strand	120-123	4	202
α-helix	131-133	3
α-helix	136-141	6

Chain C2: 5 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	27-36	10
β-strand	41-43	3	183
α-helix	45-52	8
β-strand	59-61	3	183
α-helix	70-79	10
α-helix	94-101	8
β-strand	121-123	3	183
α-helix	131-138	8

Chain c3: 6 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	30-42	13
α-helix	47-57	11
β-strand	58	1	217
α-helix	71-77	7
α-helix	86-102	17
α-helix	109-131	23
α-helix	145-147	3

Chain C3: 7 helices, 0 β-strands

Element	Residues	Length
α-helix	30-42	13
α-helix	47-53	7
α-helix	63-66	4
α-helix	71-76	6
α-helix	86-104	19
α-helix	109-131	23
α-helix	143-150	8

145 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
18S ribosomal RNA	2, 6	RNA	1800	Saccharomyces cerevisiae S288c
40S ribosomal protein S0-A	S0, s0	protein	251	Saccharomyces cerevisiae S288c	P32905 (AlphaFold model)
40S ribosomal protein S1-A	S1, s1	protein	254	Saccharomyces cerevisiae S288c	P33442 (AlphaFold model)
40S ribosomal protein S2	S2, s2	protein	253	Saccharomyces cerevisiae S288c	P25443 (AlphaFold model)
40S ribosomal protein S3	S3, s3	protein	239	Saccharomyces cerevisiae S288c	P05750 (AlphaFold model)
40S ribosomal protein S4-A	S4, s4	protein	260	Saccharomyces cerevisiae S288c	P0CX35
40S ribosomal protein S5	S5, s5	protein	224	Saccharomyces cerevisiae S288c	P26783
40S ribosomal protein S6-A	S6, s6	protein	236	Saccharomyces cerevisiae S288c	P0CX37
40S ribosomal protein S7-A	S7, s7	protein	189	Saccharomyces cerevisiae S288c	P26786
40S ribosomal protein S8-A	S8, s8	protein	200	Saccharomyces cerevisiae S288c	P0CX39
40S ribosomal protein S9-A	S9, s9	protein	196	Saccharomyces cerevisiae S288c	O13516
40S ribosomal protein S10-A	C0	protein	105	Saccharomyces cerevisiae S288c	Q08745

72 more molecules are not listed.

Sequence of entity 1 (2, 6), FASTA

>5FCI_1 18S ribosomal RNA (chains 2, 6)
UAUCUGGUUGAUCCUGCCAGUAGUCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCU
AAGUAUAAGCAAUUUAUACAGUGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUCGUUUAU
UUGAUAGUUCCUUUACUACAUGGUAUAACUGUGGUAAUUCUAGAGCUAAUACAUGCUUAA
AAUCUCGACCCUUUGGAAGAGAUGUAUUUAUUAGAUAAAAAAUCAAUGUCUUCGGACUCU
UUGAUGAUUCAUAAUAACUUUUCGAAUCGCAUGGCCUUGUGCUGGCGAUGGUUCAUUCAA
AUUUCUGCCCUAUCAACUUUCGAUGGUAGGAUAGUGGCCUACCAUGGUUUCAACGGGUAA
CGGGGAAUAAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAAACGGCUACCACAUCCAAGGA
AGGCAGCAGGCGCGCAAAUUACCCAAUCCUAAUUCAGGGAGGUAGUGACAAUAAAUAACG
AUACAGGGCCCAUUCGGGUCUUGUAAUUGGAAUGAGUACAAUGUAAAUACCUUAACGAGG
AACAAUUGGAGGGCAAGUCUGGUGCCAGCAGCCGCGGUAAUUCCAGCUCCAAUAGCGUAU
AUUAAAGUUGUUGCAGUUAAAAAGCUCGUAGUUGAACUUUGGGCCCGGUUGGCCGGUCCG
AUUUUUUCGUGUACUGGAUUUCCAACGGGGCCUUUCCUUCUGGCUAACCUUGAGUCCUUG
UGGCUCUUGGCGAACCAGGACUUUUACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCGUA
UUGCUCGAAUAUAUUAGCAUGGAAUAAUAGAAUAGGACGUUUGGUUCUAUUUUGUUGGUU
UCUAGGACCAUCGUAAUGAUUAAUAGGGACGGUCGGGGGCAUCAGUAUUCAAUUGUCAGA
GGUGAAAUUCUUGGAUUUAUUGAAGACUAACUACUGCGAAAGCAUUUGCCAAGGACGUUU
UCAUUAAUCAAGAACGAAAGUUAGGGGAUCGAAGAUGAUCAGAUACCGUCGUAGUCUUAA
CCAUAAACUAUGCCGACUAGGGAUCGGGUGGUGUUUUUUUAAUGACCCACUCGGCACCUU
ACGAGAAAUCAAAGUCUUUGGGUUCUGGGGGGAGUAUGGUCGCAAGGCUGAAACUUAAAG
GAAUUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGG
GAAACUCACCAGGUCCAGACACAAUAAGGAUUGACAGAUUGAGAGCUCUUUCUUGAUUUU
GUGGGUGGUGGUGCAUGGCCGUUCUUAGUUGGUGGAGUGAUUUGUCUGCUUAAUUGCGAU
AACGAACGAGACCUUAACCUACUAAAUAGUGGUGCUAGCAUUUGCUGGUUAUCCACUUCU
UAGAGGGACUAUCGGUUUCAAGCCGAUGGAAGUUUGAGGCAAUAACAGGUCUGUGAUGCC
CUUAGACGUUCUGGGCCGCACGCGCGCUACACUGACGGAGCCAGCGAGUCUAACCUUGGC
CGAGAGGUCUUGGUAAUCUUGUGAAACUCCGUCGUGCUGGGGAUAGAGCAUUGUAAUUAU
UGCUCUUCAACGAGGAAUUCCUAGUAAGCGCAAGUCAUCAGCUUGCGUUGAUUACGUCCC
UGCCCUUUGUACACACCGCCCGUCGCUAGUACCGAUUGAAUGGCUUAGUGAGGCCUCAGG
AUCUGCUUAGAGAAGGGGGCAACUCCAUCUCAGAGCGGAGAAUUUGGACAAACUUGGUCA
UUUAGAGGAACUAAAAGUCGUAACAAGGUUUCCGUAGGUGAACCUGCGGAAGGAUCAUUA

Sequence of entity 2 (S0, s0), FASTA

>5FCI_2 40S ribosomal protein S0-A (chains S0, s0)
SLPATFDLTPEDAQLLLAANTHLGARNVQVHQEPYVFNARPDGVHVINVGKTWEKLVLAA
RIIAAIPNPEDVVAISSRTFGQRAVLKFAAHTGATPIAGRFTPGSFTNYITRSFKEPRLV
IVTDPRSDAQAIKEASYVNIPVIALTDLDSPSEFVDVAIPCNNRGKHSIGLIWYLLAREV
LRLRGALVDRTQPWSIMPDLYFYRDPEEVEQQVAEEATTEEAGEEEAKEEVTEEQAEATE
WAEENADNVEW

Sequence of entity 3 (S1, s1), FASTA

>5FCI_3 40S ribosomal protein S1-A (chains S1, s1)
AVGKNKRLSKGKKGQKKRVVDPFTRKEWFDIKAPSTFENRNVGKTLVNKSTGLKSASDAL
KGRVVEVCLADLQGSEDHSFRKIKLRVDEVQGKNLLTNFHGMDFTTDKLRSMVRKWQTLI
EANVTVKTSDDYVLRIFAIAFTRKQANQVKRHSYAQSSHIRAIRKVISEILTKEVQGSTL
AQLTSKLIPEVINKEIENATKDIFPLQNIHVRKVKLLKQPKFDVGALMALHGEGSGEEKG
KKVTGFKDEVLETV

Sequence of entity 4 (S2, s2), FASTA

>5FCI_4 40S ribosomal protein S2 (chains S2, s2)
SAPEAQQQKRGGFGGRNRGRPNRRGPRNTEEKGWVPVTKLGRLVKAGKITTIEEIFLHSL
PVKEFQIIDTLLPGLQDEVMNIKPVQKQTRAGQRTRFKAVVVVGDSNGHVGLGIKTAKEV
AGAIRAGIIIAKLSVIPIRRGYWGTNLGQPHSLATKTTGKCGSVTVRLIPAPRGSGIVAS
PAVKKLLQLAGVEDVYTQSNGKTRTLENTLKAAFVAIGNTYGFLTPNLWAEQPLPVSPLD
IYSDEASAQKKRF

Sequence of entity 5 (S3, s3), FASTA

>5FCI_5 40S ribosomal protein S3 (chains S3, s3)
VALISKKRKLVADGVFYAELNEFFTRELAEEGYSGVEVRVTPTKTEVIIRATRTQDVLGE
NGRRINELTLLVQKRFKYAPGTIVLYAERVQDRGLSAVAQAESMKFKLLNGLAIRRAAYG
VVRYVMESGAKGCEVVVSGKLRAARAKAMKFADGFLIHSGQPVNDFIDTATRHVLMRQGV
LGIKVKIMRDPAKSRTGPKALPDAVTIIEPKEEEPILAPSVKDYRPAEETEAQAEPVEA

Sequence of entity 6 (S4, s4), FASTA

>5FCI_6 40S ribosomal protein S4-A (chains S4, s4)
ARGPKKHLKRLAAPHHWLLDKLSGCYAPRPSAGPHKLRESLPLIVFLRNRLKYALNGREV
KAILMQRHVKVDGKVRTDTTYPAGFMDVITLDATNENFRLVYDVKGRFAVHRITDEEASY
KLGKVKKVQLGKKGVPYVVTHDGRTIRYPDPNIKVNDTVKIDLASGKITDFIKFDAGKLV
YVTGGRNLGRIGTIVHKERHDGGFDLVHIKDSLDNTFVTRLNNVFVIGEQGKPYISLPKG
KGIKLSIAEERDRRRAQQGL

Sequence of entity 7 (S5, s5), FASTA

>5FCI_7 40S ribosomal protein S5 (chains S5, s5)
SDTEAPVEVQEDFEVVEEFTPVVLATPIPEEVQQAQTEIKLFNKWSFEEVEVKDASLVDY
VQVRQPIFVAHTAGRYANKRFRKAQCPIIERLTNSLMMNGRNNGKKLKAVRIIKHTLDII
NVLTDQNPIQVVVDAITNTGPREDTTRVGGGGAARRQAVDVSPLRRVNQAIALLTIGARE
AAFRNIKTIAETLAEELINAAKGSSTSYAIKKKDELERVAKSNR

Sequence of entity 8 (S6, s6), FASTA

>5FCI_8 40S ribosomal protein S6-A (chains S6, s6)
MKLNISYPVNGSQKTFEIDDEHRIRVFFDKRIGQEVDGEAVGDEFKGYVFKISGGNDKQG
FPMKQGVLLPTRIKLLLTKNVSCYRPRRDGERKRKSVRGAIVGPDLAVLALVIVKKGEQE
LEGLTDTTVPKRLGPKRANNIRKFFGLSKEDDVRDFVIRREVTKGEKTYTKAPKIQRLVT
PQRLQRKRHQRALKVRNAQAQREAAAEYAQLLAKRLSERKAEKAEIRKRRASSLKA

Sequence of entity 9 (S7, s7), FASTA

>5FCI_9 40S ribosomal protein S7-A (chains S7, s7)
SAPQAKILSQAPTELELQVAQAFVELENSSPELKAELRPLQFKSIREIDVAGGKKALAIF
VPVPSLAGFHKVQTKLTRELEKKFQDRHVIFLAERRILPKPSRTSRQVQKRPRSRTLTAV
HDKILEDLVFPTEIVGKRVRYLVGGNKIQKVLLDSKDVQQIDYKLESFQAVYNKLTGKQI
VFEIPSETH

Sequence of entity 10 (S8, s8), FASTA

>5FCI_10 40S ribosomal protein S8-A (chains S8, s8)
MGISRDSRHKRSATGAKRAQFRKKRKFELGRQPANTKIGAKRIHSVRTRGGNKKYRALRI
ETGNFSWASEGISKKTRIAGVVYHPSNNELVRTNTLTKAAIVQIDATPFRQWFEAHYGQT
LGKKKNVKEEETVAKSKNAERKWAARAASAKIESSVESQFSAGRLYACISSRPGQSGRCD
GYILEGEELAFYLRRLTAKK

Sequence of entity 11 (S9, s9), FASTA

>5FCI_11 40S ribosomal protein S9-A (chains S9, s9)
PRAPRTYSKTYSTPKRPYESSRLDAELKLAGEFGLKNKKEIYRISFQLSKIRRAARDLLT
RDEKDPKRLFEGNALIRRLVRVGVLSEDKKKLDYVLALKVEDFLERRLQTQVYKLGLAKS
VHHARVLITQRHIAVGKQIVNIPSFMVRLDSEKHIDFAPTSPFGGARPGRVARRNAARKA
EASGEAADEADEADEE

Sequence of entity 12 (C0), FASTA

>5FCI_12 40S ribosomal protein S10-A (chains C0)
MLMPKEDRNKIHQYLFQEGVVVAKKDFNQAKHEEIDTKNLYVIKALQSLTSKGYVKTQFS
WQYYYYTLTEEGVEYLREYLNLPRHIVPGTYIQERNPTQRPQRRY

Ligands and cofactors

ID	Name	Formula	Copies
OHX	osmium (III) hexammine	H12 N6 Os	1089
MG	Magnesium ion	Mg	1198
ZN	Zinc ion	Zn	16

Primary citation

Crystal Structures of the uL3 Mutant Ribosome: Illustration of the Importance of Ribosomal Proteins for Translation Efficiency. Mailliot, J., Garreau de Loubresse, N., Yusupova, G. et al. J Mol Biol (2016) 428:2195-2202. DOI 10.1016/j.jmb.2016.02.013 · PubMed

Other PDB entries of the same protein (UniProt P32905 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9PN5 1.75 Å, Composite map of hypomethylated 80S ribosome treated with hygromycin B
8CCS 1.97 Å, 80S S. cerevisiae ribosome with ligands in hybrid-1 pre-translocation (PRE-H1) complex
8CDR 2.04 Å, Translocation intermediate 2 (TI-2) of 80S S. cerevisiae ribosome with ligands and eEF2…
8CEH 2.05 Å, Translocation intermediate 4 (TI-4) of 80S S. cerevisiae ribosome with ligands and eEF2…
8EVT 2.2 Å, Hypopseudouridylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome…
8T2Y 2.2 Å, Hypomethylated yeast 80S bound with cycloheximide, P-site tRNA, and A-site tRNA,…
8CGN 2.28 Å, Non-rotated 80S S. cerevisiae ribosome with ligands
8ZHC 2.3 Å, pre-frameshift complex of yeast 80S ribosome with eRF1 and mRNA of WNV
8EVP 2.38 Å, Hypopseudouridylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome…
9DP7 2.38 Å, Hypopseudouridylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome…
7ZW0 2.4 Å, FAP-80S Complex - Rotated state
8BN3 2.4 Å, Yeast 80S, ES7s delta, eIF5A, Stm1 containing

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