5FK9: Staphylococcal enterotoxin A F47A mutant

Crystal structure of staphylococcal enterotoxin A F47A mutant in complex with a T cell receptor. Determined by X-ray diffraction at 3.1 Å resolution. Released 25 May 2016.

Method: X-ray diffraction
Resolution: 3.1 Å
Organisms: HOMO SAPIENS, STAPHYLOCOCCUS AUREUS
Chains: 3
Atoms: 4,771
Mol. weight: 77.63 kDa
Released: 25 May 2016

Explore 5FK9 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5FK9 contains 13 α-helices and 57 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 1 helix, 17 β-strands

Element	Residues	Length	Sheet
β-strand	5-8	4	1
β-strand	11-15	5	2
β-strand	20-26	7	1
β-strand	32-38	7	2
β-strand	44-49	6	2
β-strand	53-57	5	1
β-strand	60-65	6	1
β-strand	70-75	6	1
α-helix	80-82	3
β-strand	84-91	8	2
β-strand	100-102	3	2
β-strand	106-111	6	2
β-strand	120-126	7	3
β-strand	133-138	6	3
β-strand	155-156	2	3
β-strand	160-163	4	3
β-strand	170-177	8	3
β-strand	199	1	3

Chain B: 7 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	6-8	3	4
β-strand	11-15	5	5
β-strand	20-25	6	4
β-strand	32-38	7	5
α-helix	43-44	2
β-strand	45-51	7	5
β-strand	54-58	5	5
β-strand	66-69	4	4
β-strand	76-80	5	4
β-strand	90-96	7	5
β-strand	102-104	3	5
β-strand	108-113	6	5
α-helix	116-118	3
β-strand	120	1	6
β-strand	123-128	6	3
α-helix	129-130	2
α-helix	131-136	6
β-strand	139-149	11	3
β-strand	150	1	6
β-strand	154-160	7	7
β-strand	163-165	3	7
β-strand	169-171	3	3
α-helix	175	1
β-strand	176-177	2	3
β-strand	187-196	10	3
α-helix	197-201	5
β-strand	206-213	8	7
β-strand	216	1	8
α-helix	227-228	2
β-strand	230	1	8
β-strand	233-239	7	7

Chain C: 5 helices, 16 β-strands

Element	Residues	Length	Sheet
α-helix	22-30	9
β-strand	36-41	6	9
β-strand	52-54	3	10
β-strand	66-70	5	10
α-helix	74-81	8
β-strand	85-89	5	9
β-strand	92	1	10
β-strand	104-108	5	10
β-strand	111-113	3	9
β-strand	123-124	2	11
β-strand	127-131	5	12
β-strand	134-137	4	12
β-strand	144-145	2	11
β-strand	149-151	3	13
α-helix	152-166	15
β-strand	181-186	6	12
β-strand	194-197	4	12
α-helix	206-209	4
α-helix	211-215	5
β-strand	218-220	3	13
β-strand	226-231	6	12

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
T cell receptor alpha chain	A	protein	206	HOMO SAPIENS	A0A0B4J277 (AlphaFold model), P01848 (AlphaFold model)
T cell receptor beta chain	B	protein	243	HOMO SAPIENS	A0A5A3 (AlphaFold model), A0A5B9 (AlphaFold model)
Enterotoxin type A	C	protein	233	STAPHYLOCOCCUS AUREUS	P0A0L2

Sequence of entity 1 (A), FASTA

>5FK9_1 T CELL RECEPTOR ALPHA CHAIN (chains A)
MGIQVEQSPPDLILQEGANSTLRCNFSDSVNNLQWFHQNPWGQLINLFYIPSGTKQNGRL
SATTVATERYSLLYISSSQTTDSGVYFCAVDSATSGTYKYIFGTGTRLKVLANIQNPDPA
VYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKCVLDMRSMDFKSNSAVAWSNK
SDFACANAFNNSIIPEDTFFPSPESS

Sequence of entity 2 (B), FASTA

>5FK9_2 T CELL RECEPTOR BETA CHAIN (chains B)
MDTGVSQNPRHKITKRGQNVTFRCDPISEHNRLYWYRQTLGQGPEFLTYFQNEAQLEKSR
LLSDRFSAERPKGSFSTLEIQRTEQGDSAMYLCASSLGGYEQYFGPGTRLTVTEDLKNVF
PPEVAVFVPSEAEISHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVCTDPQPLKEQP
ALNDSRYALSSRLRVSATFWQDPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWG
RAD

Sequence of entity 3 (C), FASTA

>5FK9_3 ENTEROTOXIN TYPE A (chains C)
SEKSEEINEKDLRKKSELQGTALGNLKQIYYYNEKAKTENKESHDQALQHTILFKGFFTD
HSWYNDLLVDFDSKDIVDKYKGKKVDLYGAYYGYQCAGGTPNKTACMYGGVTLHDNNRLT
EEKKVPINLWLDGKQNTVPLETVKTNKKNVTVQELDLQARRYLQEKYNLYNSDVFDGKVQ
RGLIVFHTSTEPSVNYDLFGAQGQYSNTLLRIYRDNKTINSENMHIDIYLYTS

Primary citation

Two Common Structural Motifs for Tcr Recognition by Staphylococcal Enterotoxins. Rodstrom, K.E.J., Regenthal, P., Bahl, C. et al. Sci Rep (2016) 6:25796. DOI 10.1038/SREP25796 · PubMed

Other PDB entries of the same protein (UniProt A0A0B4J277 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4UDT 1.35 Å, T cell receptor (TRAV22,TRBV7-9) structure
6R2L 2.3 Å, NYBR1-A2-slskildtv
5FKA 2.4 Å, Crystal structure of staphylococcal enterotoxin E in complex with a T cell receptor
4UDU 2.5 Å, Crystal structure of staphylococcal enterotoxin E in complex with a T cell receptor
5MEN 2.81 Å, Human Leukocyte Antigen A02 presenting ILAKFLHWL, in complex with cognate T-Cell Receptor

Browse structure collections

About this viewer

MolViewer shows 5FK9 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.