Contact-dependent inhibition system from Escherichia coli NC101 - ternary CdiA/CdiI/EF-Tu complex (domains 2 and 3). Determined by X-ray diffraction at 2.35 Å resolution. Released 28 Jun 2017.
Explore 5I4Q in 3D Show helices and sheets RCSB PDB PDBe
5I4Q contains 12 α-helices and 30 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 171-172 | 2 | 1 |
| α-helix | 173 | 1 | |
| β-strand | 174-175 | 2 | 2 |
| β-strand | 179-182 | 4 | 2 |
| β-strand | 185-188 | 4 | 2 |
| α-helix | 189-197 | 9 | |
| α-helix | 203-211 | 9 | |
| β-strand | 216-219 | 4 | 2 |
| β-strand | 228-232 | 5 | 2 |
| β-strand | 235-240 | 6 | 2 |
| β-strand | 245-251 | 7 | 2 |
| α-helix | 252-254 | 3 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 4-40 | 37 | |
| α-helix | 44-66 | 23 | |
| α-helix | 74-82 | 9 | |
| α-helix | 88-99 | 12 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 212-214 | 3 | 3 |
| β-strand | 217-221 | 5 | 1 |
| β-strand | 225-231 | 7 | 1 |
| β-strand | 234 | 1 | 3 |
| β-strand | 236-238 | 3 | 4 |
| β-strand | 242-247 | 6 | 3 |
| β-strand | 249-255 | 7 | 3 |
| β-strand | 256-261 | 6 | 1 |
| β-strand | 265-266 | 2 | 1 |
| β-strand | 268-270 | 3 | 4 |
| β-strand | 274-279 | 6 | 1 |
| α-helix | 284-286 | 3 | |
| β-strand | 289 | 1 | 5 |
| β-strand | 291 | 1 | 5 |
| β-strand | 292-294 | 3 | 3 |
| β-strand | 300-311 | 12 | 6 |
| α-helix | 312-313 | 2 | |
| α-helix | 314-316 | 3 | |
| β-strand | 323 | 1 | 7 |
| β-strand | 330-333 | 4 | 6 |
| β-strand | 336-343 | 8 | 6 |
| α-helix | 344-345 | 2 | |
| β-strand | 351 | 1 | 7 |
| β-strand | 356-369 | 14 | 6 |
| β-strand | 374-379 | 6 | 6 |
| β-strand | 382-392 | 11 | 6 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Contact-dependent inhibitor A | A | protein | 92 | Escherichia coli NC101 | P0DSI1 |
| Contact-dependent inhibitor I | B | protein | 114 | Escherichia coli NC101 | P0DSM8 (AlphaFold model) |
| Elongation factor Tu | C | protein | 217 | Escherichia coli | P0CE47 (AlphaFold model) |
>5I4Q_1 Contact-dependent inhibitor A (chains A) LSYLGIGKKISFDGDFYTVDGMKFSKSYYEKLWEQGRPAPFVQAREVLNSNPKIEPDPRG APGYLRYEGAGLEMIYNPKTGQVGHIQPVKVK
>5I4Q_2 Contact-dependent inhibitor I (chains B) MDIWPEFQRDLEMYRDVVLSIKRNLRLYEECIESLVHQIGSTNFDNAQPLFDDLFRMQSE LATMLYKYEYKPGKRIQDLIYHLDRDDFYSRKYWHKKFSDGLAWPEAGHHHHHH
>5I4Q_3 Elongation factor Tu (chains C) ALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG TIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNI KMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG
Structure of a novel antibacterial toxin that exploits elongation factor Tu to cleave specific transfer RNAs. Michalska, K., Gucinski, G.C., Garza-Sanchez, F. et al. Nucleic Acids Res (2017) 45:10306-10320. DOI 10.1093/nar/gkx700 · PubMed
Other PDB entries of the same protein (UniProt P0DSI1), best resolution first:
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