5JR7: ACRDYS heterodimer [RIa(92-365):C] of PKA

Crystal structure of an ACRDYS heterodimer [RIa(92-365):C] of PKA. Determined by X-ray diffraction at 3.56 Å resolution. Released 15 Mar 2017.

Method: X-ray diffraction
Resolution: 3.56 Å
Organisms: Mus musculus, Bos taurus
Chains: 4
Atoms: 9,665
Mol. weight: 144.16 kDa
Ligands: ADP
Released: 15 Mar 2017

Explore 5JR7 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5JR7 contains 57 α-helices and 68 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 19 helices, 14 β-strands

Element	Residues	Length	Sheet
α-helix	14-31	18
α-helix	40-42	3
β-strand	43-51	9	1
β-strand	55-62	8	1
β-strand	67-75	9	1
α-helix	76-81	6
α-helix	85-97	13
β-strand	103	1	2
α-helix	104-105	2
β-strand	106-111	6	1
β-strand	115-121	7	1
α-helix	122	1
β-strand	127	1	2
α-helix	128-135	8
α-helix	140-159	20
β-strand	162-163	2	3
α-helix	169-171	3
β-strand	172-174	3	2
β-strand	180-182	3	2
β-strand	189-190	2	3
β-strand	195	1	4
β-strand	199-200	2	5
α-helix	202-204	3
α-helix	207-210	4
β-strand	215	1	4
α-helix	219-233	15
α-helix	243-252	10
α-helix	263-272	10
α-helix	289-292	4
α-helix	295-297	3
α-helix	302-306	5
α-helix	311-312	2
α-helix	341	1

Chain B: 12 helices, 19 β-strands

Element	Residues	Length	Sheet
α-helix	94-97	4
β-strand	98-99	2	5
α-helix	105-110	6
α-helix	120-130	11
α-helix	134-137	4
α-helix	141-150	10
β-strand	152-156	5	6
β-strand	161-163	3	7
β-strand	168	1	8
α-helix	169	1
β-strand	171-177	7	6
β-strand	180-184	5	7
β-strand	187-192	6	7
β-strand	197-198	2	6
α-helix	200-203	4
β-strand	208	1	8
β-strand	212-215	4	7
β-strand	219-225	7	6
α-helix	226-249	24
α-helix	252-254	3
α-helix	259-268	10
β-strand	270-274	5	9
β-strand	279-281	3	9
β-strand	286-287	2	10
β-strand	289-302	14	9
β-strand	311-316	6	9
β-strand	321-322	2	9
α-helix	325-328	4
β-strand	331-332	2	10
β-strand	336-349	14	9
α-helix	350-356	7

Chain C: 15 helices, 15 β-strands

Element	Residues	Length	Sheet
α-helix	16-31	16
α-helix	40-42	3
β-strand	43	1	11
β-strand	47-51	5	11
β-strand	56-62	7	11
β-strand	67-75	9	11
α-helix	76-81	6
α-helix	85-95	11
β-strand	103	1	12
β-strand	106-110	5	11
β-strand	115-121	7	11
β-strand	127	1	12
α-helix	128-135	8
α-helix	140-159	20
β-strand	162-163	2	13
α-helix	169-171	3
β-strand	172-174	3	12
β-strand	180-182	3	12
β-strand	189-190	2	13
β-strand	195	1	14
β-strand	199-200	2	15
α-helix	202-204	3
α-helix	207-210	4
β-strand	215	1	14
α-helix	219-233	15
α-helix	243-250	8
α-helix	263-272	10
α-helix	289-292	4
α-helix	295-297	3
α-helix	302-306	5

Chain D: 11 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	98-99	2	15
α-helix	105-108	4
α-helix	120-123	4
α-helix	125-130	6
α-helix	134-137	4
α-helix	141-150	10
β-strand	154-156	3	16
β-strand	160-163	4	17
β-strand	168	1	18
α-helix	169	1
β-strand	171-175	5	16
β-strand	180-184	5	17
β-strand	187-192	6	17
α-helix	201-204	4
β-strand	208	1	18
β-strand	212-215	4	17
β-strand	219-225	7	16
α-helix	226-250	25
α-helix	259-266	8
β-strand	270-274	5	19
β-strand	279	1	19
β-strand	286-287	2	20
β-strand	289	1	21
β-strand	291-303	13	19
β-strand	310-316	7	19
β-strand	321-322	2	19
α-helix	325-328	4
β-strand	331-332	2	20
β-strand	336-347	12	19
β-strand	349	1	21
α-helix	350-356	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
cAMP-dependent protein kinase catalytic subunit alpha	A, C	protein	350	Mus musculus	P05132 (AlphaFold model)
cAMP-dependent protein kinase type I-alpha regulatory subunit	B, D	protein	275	Bos taurus	P00514 (AlphaFold model)

Sequence of entity 1 (A, C), FASTA

>5JR7_1 cAMP-dependent protein kinase catalytic subunit alpha (chains A, C)
GNAAAAKKGSEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLV
KHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVM
EYVAGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYI
QVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFA
DQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATT
DWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF

Sequence of entity 2 (B, D), FASTA

>5JR7_2 cAMP-dependent protein kinase type I-alpha regulatory subunit (chains B, D)
GRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDA
MFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIYGTPRA
ATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALE
PVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMN
RPRAATVVARGPLKCVKLDRPRFERVLGPCSDILK

Ligands and cofactors

ID	Name	Formula	Copies
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	2

Primary citation

Structure of a PKA RI alpha Recurrent Acrodysostosis Mutant Explains Defective cAMP-Dependent Activation. Bruystens, J.G., Wu, J., Fortezzo, A. et al. J Mol Biol (2016) 428:4890-4904. DOI 10.1016/j.jmb.2016.10.033 · PubMed

Other PDB entries of the same protein (UniProt P05132 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1RDQ 1.26 Å, Hydrolysis of ATP in the crystal of Y204A mutant of cAMP-dependent protein kinase
4DFX 1.35 Å, Crystal structure of myristoylated K7C catalytic subunit of cAMP-dependent protein…
4HPU 1.55 Å, Crystal structure of the catalytic subunit of cAMP-dependent protein kinase displaying…
4IAI 1.55 Å, X-ray Structure of cAMP dependent protein kinase A in complex with high Ca2+…
9PC1 1.55 Å, Co-crystal structure of the cAMP-dependent protein kinase catalytic subunit alpha with…
3FJQ 1.6 Å, Crystal structure of cAMP-dependent protein kinase catalytic subunit alpha in complex…
4IAK 1.6 Å, Low temperature X-ray structure of cAMP dependent protein kinase A in complex with high…
3IDB 1.62 Å, Crystal structure of (108-268)RIIb:C holoenzyme of cAMP-dependent protein kinase
4IB1 1.63 Å, Structure of cAMP dependent protein kinase A in complex with high K+ concentration, ADP…
7V0G 1.63 Å, Structure of cAMP-dependent protein kinase using a MD-MX procedure, produced using 1.63…
3QAL 1.7 Å, Crystal Structure of Arg280Ala mutant of Catalytic subunit of cAMP-dependent Protein…
3X2W 1.7 Å, Michaelis complex of cAMP-dependent Protein Kinase Catalytic Subunit

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