5M3X: Human angiotensin I-deleted angiotensinogen

Crystal structure of human angiotensin I-deleted angiotensinogen. Determined by X-ray diffraction at 2.63 Å resolution. Released 20 Dec 2017.

Method: X-ray diffraction
Resolution: 2.63 Å
Organism: Homo sapiens
Chains: 2
Atoms: 5,923
Mol. weight: 98.73 kDa
Released: 20 Dec 2017

Explore 5M3X in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5M3X contains 34 α-helices and 40 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 17 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	33-34	2	1
α-helix	35-36	2
β-strand	37-38	2	2
α-helix	44-46	3
α-helix	48-60	13
α-helix	64-86	23
α-helix	91-94	4
β-strand	96-97	2	2
β-strand	99-101	3	3
α-helix	103-115	13
α-helix	119-129	11
α-helix	133-135	3
α-helix	144-159	16
β-strand	170-180	11	2
α-helix	184-185	2
β-strand	186-187	2	1
α-helix	188-197	10
β-strand	200-205	6	2
α-helix	211-226	16
β-strand	243-254	12	2
β-strand	258-260	3	3
β-strand	265-267	3	4
β-strand	275-277	3	4
β-strand	279-291	13	3
β-strand	296-302	7	3
β-strand	307-314	8	3
α-helix	317-319	3
α-helix	320-327	8
β-strand	340-349	10	3
β-strand	351-358	8	2
α-helix	359-362	4
α-helix	364-366	3
α-helix	368-371	4
β-strand	388-400	13	2
β-strand	419-422	4	3
β-strand	427-433	7	3
β-strand	438-445	8	3

Chain B: 17 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	33-34	2	5
α-helix	35-37	3
β-strand	38	1	6
α-helix	44-46	3
α-helix	48-60	13
α-helix	64-86	23
α-helix	91-93	3
β-strand	96-97	2	6
β-strand	99-101	3	7
α-helix	103-115	13
α-helix	119-129	11
α-helix	134-136	3
α-helix	144-159	16
β-strand	170-180	11	6
α-helix	184-185	2
β-strand	186-187	2	5
α-helix	188-197	10
β-strand	200-205	6	6
α-helix	211-226	16
β-strand	243-254	12	6
β-strand	258-260	3	7
β-strand	265-267	3	8
β-strand	275-277	3	8
β-strand	279-291	13	7
β-strand	296-302	7	7
β-strand	307-314	8	7
α-helix	317-319	3
α-helix	320-327	8
β-strand	340-349	10	7
β-strand	351-358	8	6
α-helix	359-362	4
α-helix	364-366	3
α-helix	368-373	6
β-strand	388-400	13	6
β-strand	419-422	4	7
β-strand	427-433	7	7
β-strand	438-445	8	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Angiotensinogen	A, B	protein	448	Homo sapiens	P01019 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>5M3X_1 Angiotensinogen (chains A, B)
VIHNESTCEQLAKANAGKPKDPTFIPAPIQAKTSPVDEKALQDQLVLVAAKLDTEDKLRA
AMVGMLANFLGFRIYGMHSELWGVVHGATVLSPTAVFGTLASLYLGALDHTADRLQAILG
VPWKDKQCTSRLDAHKVLSALQAVQGLLVAQGRADSQAQLLLSTVVGVFTAPGLHLKQPF
VQGLALYTPVVLPRSLDFTELDVAAEKIDRFMQAVTGWKTGSSLMGASVDSTLAFNTYVH
FQGKMKGFSLLAEPQEFWVDQSTSVSVPMLSGMGTFQHWSDIQDQFSVTQVPFTESASLL
LIQPHYASDLDKVEGLTFQQNSLNWMKKLSPRTIHLTMPQLVLQGSYDLQDLLAQAELPA
ILHTELNLQKLSNDRIRVGEVLNSIFFELEADEREPTESTQQLNKPEVLEVTLNRPFLFA
VYDQSATALHFLGRVANPLSTAHHHHHH

Primary citation

Structural basis for the specificity of renin-mediated angiotensinogen cleavage. Yan, Y., Zhou, A., Carrell, R.W. et al. J Biol Chem (2019) 294:2353-2364. DOI 10.1074/jbc.RA118.006608 · PubMed

Other PDB entries of the same protein (UniProt P01019 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5NJ9 1.25 Å, E. coli Microcin-processing metalloprotease TldD/E with DRVY angiotensin fragment bound
5NJA 1.4 Å, E. coli Microcin-processing metalloprotease TldD/E with angiotensin analogue bound
3WOO 1.8 Å, Crystal structure of the DAP BII hexapeptide complex I
4AA1 1.99 Å, Crystal structure of ANCE in complex with Angiotensin-II
4APH 1.99 Å, Human angiotensin-converting enzyme in complex with angiotensin-II
4FYS 2.01 Å, Human aminopeptidase N (CD13) in complex with angiotensin IV
3WOR 2.1 Å, Crystal structure of the DAP BII octapeptide complex
5M3Y 2.3 Å, Crystal structure of human glycosylated angiotensinogen
5E2Q 2.4 Å, Structure of human DPP3 in complex with angiotensin-II
6I3F 2.55 Å, Crystal structure of the complex of human angiotensinogen and renin at 2.55 Angstrom
6OS0 2.9 Å, Structure of synthetic nanobody-stabilized angiotensin II type 1 receptor bound to…
6I3I 2.97 Å, Crystal structure of reactive center loop (RCL) cleaved angiotensinogen

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