5OED: Human Rab32:GDP

Human Rab32:GDP in complex with Salmonella GtgE C45A mutant. Determined by X-ray diffraction at 2.9 Å resolution. Released 10 Jan 2018.

Method: X-ray diffraction
Resolution: 2.9 Å
Organisms: Salmonella choleraesuis, Homo sapiens
Chains: 2
Atoms: 2,948
Mol. weight: 47.73 kDa
Ligands: GDP, MG
Released: 10 Jan 2018

Explore 5OED in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5OED contains 15 α-helices and 17 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	24-25	2	1
β-strand	34	1	2
β-strand	41	1	2
α-helix	45-54	10
α-helix	64-67	4
β-strand	81-82	2	1
α-helix	83-92	10
β-strand	95-97	3	1
α-helix	98-100	3
α-helix	102-113	12
α-helix	118-134	17
β-strand	138-143	6	1
β-strand	150-159	10	1
β-strand	164-168	5	1
β-strand	176-179	4	1
α-helix	180-191	12
β-strand	204-208	5	1
α-helix	213-215	3

Chain B: 7 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	22-32	11	3
α-helix	38-47	10
β-strand	57-58	2	1
β-strand	63-67	5	3
β-strand	73-81	9	3
α-helix	83-87	5
α-helix	93-96	4
β-strand	101-107	7	3
α-helix	111-127	17
α-helix	134-137	4
β-strand	138-143	6	3
α-helix	161-165	5
β-strand	169-172	4	3
α-helix	181-194	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
GtgE	A	protein	230	Salmonella choleraesuis	Q6EAT3 (AlphaFold model)
Ras-related protein Rab-32	B	protein	183	Homo sapiens	Q13637 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>5OED_1 GtgE (chains A)
GHMLRHIQNSLGSVYRSNTATPQGQIIHHRNFQSQFDTTGNTLYNNAWVCSLNVIKSRDG
NNYSALEDITSDNQAFNNILEGIDIIECENLLKEMNVQKIPESSLFTNIKEALQAEVFNS
TVEDDFESFISYELQNHGPLMLIRPSLGSECLHAECIVGYDSEVKKVLIYDSMNTSPEWQ
SNIDVYDKLTLAFNDKYKNEDCSICGLYYDGVYEPKPLHSSSWKDWCTIL

Sequence of entity 2 (B), FASTA

>5OED_2 Ras-related protein Rab-32 (chains B)
METREHLFKVLVIGELGVGKTSIIKRYVHQLFSQHYRATIGVDFALKVLNWDSRTLVRLQ
LWDIAGQERFGNMTRVYYKEAVGAFVVFDISRSSTFEAVLKWKSDLDSKVHLPNGSPIPA
VLLANKCDQNKDSSQSPSQVDQFCKEHGFAGWFETSAKDNINIEEAARFLVEKILVNHQS
FPN

Ligands and cofactors

ID	Name	Formula	Copies
GDP	Guanosine-5'-diphosphate	C10 H15 N5 O11 P2	1
MG	Magnesium ion	Mg	1

Primary citation

The protease GtgE from Salmonella exclusively targets inactive Rab GTPases. Wachtel, R., Brauning, B., Mader, S.L. et al. Nat Commun (2018) 9:44-44. DOI 10.1038/s41467-017-02110-1 · PubMed

Other PDB entries of the same protein (UniProt Q6EAT3 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5OEC 2.3 Å, Human Rab32 (18-201):GDP in complex with Salmonella GtgE (21-214) C45A mutant

Browse structure collections

About this viewer

MolViewer shows 5OED directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.