Crystal structure of USP30 (C77A) in complex with Lys6-linked diubiquitin. Determined by X-ray diffraction at 2.8 Å resolution. Released 20 Sept 2017.
Explore 5OHP in 3D Show helices and sheets RCSB PDB PDBe
5OHP contains 25 α-helices and 38 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 66-68 | 3 | |
| β-strand | 69-70 | 2 | 1 |
| β-strand | 72 | 1 | 2 |
| α-helix | 77-87 | 11 | |
| α-helix | 90-100 | 11 | |
| α-helix | 101-103 | 3 | |
| α-helix | 116-128 | 13 | |
| α-helix | 130-131 | 2 | |
| β-strand | 137-138 | 2 | 1 |
| α-helix | 141-147 | 7 | |
| β-strand | 160 | 1 | 3 |
| α-helix | 162-178 | 17 | |
| β-strand | 226-234 | 9 | 4 |
| α-helix | 239 | 1 | |
| β-strand | 240 | 1 | 4 |
| α-helix | 241-243 | 3 | |
| β-strand | 244-248 | 5 | 4 |
| β-strand | 251-254 | 4 | 5 |
| α-helix | 255 | 1 | |
| α-helix | 257-259 | 3 | |
| α-helix | 262-263 | 2 | |
| β-strand | 264-265 | 2 | 6 |
| α-helix | 266-274 | 9 | |
| β-strand | 276-283 | 8 | 4 |
| α-helix | 285-288 | 4 | |
| β-strand | 305-316 | 12 | 4 |
| β-strand | 320-326 | 7 | 5 |
| β-strand | 328-330 | 3 | 7 |
| β-strand | 336-338 | 3 | 7 |
| α-helix | 342 | 1 | |
| β-strand | 343-344 | 2 | 6 |
| β-strand | 348-350 | 3 | 5 |
| α-helix | 352-354 | 3 | |
| β-strand | 355 | 1 | 4 |
| β-strand | 435-446 | 12 | 5 |
| β-strand | 451-458 | 8 | 5 |
| α-helix | 459-462 | 4 | |
| β-strand | 473-477 | 5 | 5 |
| β-strand | 480-484 | 5 | 5 |
| α-helix | 486-490 | 5 | |
| β-strand | 494-501 | 8 | 5 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 2-6 | 5 | 8 |
| β-strand | 12-16 | 5 | 8 |
| β-strand | 22 | 1 | 9 |
| α-helix | 23-34 | 12 | |
| α-helix | 38-40 | 3 | |
| β-strand | 42-45 | 4 | 8 |
| β-strand | 48-49 | 2 | 8 |
| β-strand | 55 | 1 | 9 |
| β-strand | 66-70 | 5 | 8 |
| β-strand | 75 | 1 | 3 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 2-7 | 6 | 10 |
| β-strand | 10 | 1 | 2 |
| β-strand | 12-16 | 5 | 10 |
| β-strand | 22 | 1 | 11 |
| α-helix | 23-34 | 12 | |
| α-helix | 38-40 | 3 | |
| β-strand | 41-45 | 5 | 10 |
| β-strand | 48-49 | 2 | 10 |
| α-helix | 50-51 | 2 | |
| β-strand | 55 | 1 | 11 |
| α-helix | 56-59 | 4 | |
| β-strand | 66-71 | 6 | 10 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Ubiquitin carboxyl-terminal hydrolase 30 | A | protein | 336 | Homo sapiens | Q70CQ3 (AlphaFold model) |
| Polyubiquitin-B | B, C | protein | 76 | Homo sapiens | P0CG47 (AlphaFold model) |
>5OHP_1 Ubiquitin carboxyl-terminal hydrolase 30 (chains A) GPKGLVPGLVNLGNTAFMNSLLQGLSACPAFIRWLEEFTSQYSRDQKEPPSHQYLSLTLL HLLKALSCQEVTDDEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVITSSLEDERDGSG SHWKSQHPFHGRLTSNMVCKHCEHQSPVRFDTFDSLSLSIPAATWGHPLTLDHCLHHFIS SESVRDVVCDNCTKIEAKGTLNGEKVEHQRTTFVKQLKLGKLPQCLCIHLQRLSWSSHGT PLKRHEHVQFNEDLSMDEYKYHSNASTYLFRLMAVVVHHGDMHSGHFVTYRRSPPSARNP LSTSNQWLWVSDDTVRKASLQEVLSSSAYLLFYERV
>5OHP_2 Polyubiquitin-B (chains B, C) MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG
| ID | Name | Formula | Copies |
|---|---|---|---|
| ZN | Zinc ion | Zn | 1 |
Mechanism and regulation of the Lys6-selective deubiquitinase USP30. Gersch, M., Gladkova, C., Schubert, A.F. et al. Nat Struct Mol Biol (2017) 24:920-930. DOI 10.1038/nsmb.3475 · PubMed
Other PDB entries of the same protein (UniProt Q70CQ3 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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