5S5Q: Tubulin-Z396380540-complex
Tubulin-Z396380540-complex. Determined by X-ray diffraction at 2.05 Å resolution. Released 30 Jun 2021.
- Method
- X-ray diffraction
- Resolution
- 2.05 Å
- Organisms
- Bos taurus, Rattus norvegicus, Gallus gallus
- Chains
- 6
- Atoms
- 18,077
- Mol. weight
- 264.75 kDa
- Ligands
- GTP, MG, CA, GDP
- Released
- 30 Jun 2021
Explore 5S5Q in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
5S5Q contains 133 α-helices and 91 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 29 helices, 16 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 4-9 | 6 | 1 |
| α-helix | 10-28 | 19 | |
| α-helix | 48-51 | 4 | |
| β-strand | 53-55 | 3 | 2 |
| β-strand | 61-63 | 3 | 2 |
| β-strand | 65-69 | 5 | 1 |
| α-helix | 72-79 | 8 | |
| α-helix | 84-86 | 3 | |
| α-helix | 89-91 | 3 | |
| β-strand | 92-94 | 3 | 1 |
| α-helix | 103-104 | 2 | |
| α-helix | 105-109 | 5 | |
| α-helix | 111-126 | 16 | |
| β-strand | 134-140 | 7 | 1 |
| α-helix | 144 | 1 | |
| α-helix | 145-149 | 5 | |
| α-helix | 150-160 | 11 | |
| β-strand | 165-172 | 8 | 1 |
| α-helix | 175-177 | 3 | |
| α-helix | 183-194 | 12 | |
| α-helix | 195-197 | 3 | |
| β-strand | 200-205 | 6 | 1 |
| α-helix | 206-215 | 10 | |
| α-helix | 224-243 | 20 | |
| α-helix | 252-259 | 8 | |
| α-helix | 268 | 1 | |
| β-strand | 269-273 | 5 | 3 |
| β-strand | 277 | 1 | 4 |
| α-helix | 283-286 | 4 | |
| α-helix | 288-294 | 7 | |
| α-helix | 298-300 | 3 | |
| β-strand | 301 | 1 | 3 |
| α-helix | 307-309 | 3 | |
| β-strand | 312-321 | 10 | 3 |
| α-helix | 325-336 | 12 | |
| β-strand | 343 | 1 | 3 |
| β-strand | 349-356 | 8 | 3 |
| α-helix | 359-361 | 3 | |
| β-strand | 368 | 1 | 4 |
| α-helix | 369-370 | 2 | |
| β-strand | 373-381 | 9 | 3 |
| α-helix | 382-384 | 3 | |
| α-helix | 385-399 | 15 | |
| α-helix | 405-409 | 5 | |
| α-helix | 415-436 | 22 | |
Chain B: 29 helices, 20 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 2-9 | 8 | 5 |
| α-helix | 10-27 | 18 | |
| β-strand | 30 | 1 | 6 |
| β-strand | 35 | 1 | 7 |
| β-strand | 36 | 1 | 6 |
| α-helix | 42-47 | 4 | |
| α-helix | 49-51 | 3 | |
| β-strand | 53-56 | 4 | 7 |
| α-helix | 57-59 | 3 | |
| β-strand | 60-63 | 4 | 7 |
| β-strand | 65-69 | 5 | 5 |
| α-helix | 73-80 | 8 | |
| α-helix | 84-86 | 3 | |
| α-helix | 89-91 | 3 | |
| β-strand | 92-94 | 3 | 5 |
| α-helix | 103-107 | 5 | |
| α-helix | 110-127 | 18 | |
| β-strand | 131-140 | 10 | 5 |
| α-helix | 145-149 | 5 | |
| α-helix | 150-160 | 11 | |
| β-strand | 165-172 | 8 | 5 |
| α-helix | 173 | 1 | |
| β-strand | 174 | 1 | 8 |
| β-strand | 177 | 1 | 8 |
| α-helix | 183-197 | 15 | |
| β-strand | 200-205 | 6 | 5 |
| α-helix | 206-211 | 6 | |
| α-helix | 212-216 | 5 | |
| α-helix | 224-238 | 15 | |
| α-helix | 240-243 | 4 | |
| α-helix | 252-259 | 8 | |
| β-strand | 267-268 | 2 | 5 |
| β-strand | 269-273 | 5 | 9 |
| α-helix | 285-287 | 3 | |
| α-helix | 288-295 | 8 | |
| α-helix | 298-300 | 3 | |
| β-strand | 301 | 1 | 9 |
| α-helix | 307-309 | 3 | |
| β-strand | 312-320 | 9 | 9 |
| α-helix | 325-336 | 12 | |
| α-helix | 340-342 | 3 | |
| β-strand | 343 | 1 | 9 |
| β-strand | 351-356 | 6 | 9 |
| α-helix | 359-360 | 2 | |
| β-strand | 374-381 | 8 | 9 |
| α-helix | 382-384 | 3 | |
| α-helix | 385-399 | 15 | |
| α-helix | 406-409 | 4 | |
| α-helix | 415-436 | 22 | |
Chain C: 34 helices, 16 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 4-9 | 6 | 10 |
| α-helix | 10-28 | 19 | |
| α-helix | 48-51 | 4 | |
| β-strand | 53-55 | 3 | 11 |
| β-strand | 61-63 | 3 | 11 |
| β-strand | 65-69 | 5 | 10 |
| α-helix | 73-79 | 7 | |
| α-helix | 84-86 | 3 | |
| α-helix | 89-91 | 3 | |
| β-strand | 92-94 | 3 | 10 |
| α-helix | 103-104 | 2 | |
| α-helix | 105-109 | 5 | |
| α-helix | 111-113 | 3 | |
| α-helix | 115-127 | 13 | |
| β-strand | 134-140 | 7 | 10 |
| α-helix | 144 | 1 | |
| α-helix | 145-149 | 5 | |
| α-helix | 150-161 | 12 | |
| β-strand | 165-172 | 8 | 10 |
| α-helix | 175-177 | 3 | |
| α-helix | 183-194 | 12 | |
| α-helix | 195-197 | 3 | |
| β-strand | 200-205 | 6 | 10 |
| α-helix | 206-217 | 12 | |
| α-helix | 224-243 | 20 | |
| α-helix | 252-259 | 8 | |
| α-helix | 268 | 1 | |
| β-strand | 269-273 | 5 | 12 |
| β-strand | 277 | 1 | 13 |
| α-helix | 278-281 | 4 | |
| α-helix | 288-295 | 8 | |
| α-helix | 298-300 | 3 | |
| β-strand | 301 | 1 | 12 |
| α-helix | 307-309 | 3 | |
| β-strand | 312-321 | 10 | 12 |
| α-helix | 325-338 | 14 | |
| α-helix | 342 | 1 | |
| β-strand | 343 | 1 | 12 |
| α-helix | 344 | 1 | |
| α-helix | 350-351 | 2 | |
| β-strand | 352-356 | 5 | 12 |
| α-helix | 359-360 | 2 | |
| β-strand | 368 | 1 | 13 |
| α-helix | 369-370 | 2 | |
| β-strand | 373-381 | 9 | 12 |
| α-helix | 382-384 | 3 | |
| α-helix | 385-399 | 15 | |
| α-helix | 405-410 | 6 | |
| α-helix | 415-435 | 21 | |
| α-helix | 438-439 | 2 | |
Chain D: 25 helices, 17 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 4-9 | 6 | 14 |
| α-helix | 10-28 | 19 | |
| β-strand | 30 | 1 | 15 |
| β-strand | 36 | 1 | 15 |
| α-helix | 41-45 | 3 | |
| α-helix | 49-51 | 3 | |
| β-strand | 53-56 | 4 | 16 |
| β-strand | 60-63 | 4 | 16 |
| β-strand | 65-69 | 5 | 14 |
| α-helix | 72-80 | 9 | |
| α-helix | 84-86 | 3 | |
| α-helix | 89-91 | 3 | |
| β-strand | 92-94 | 3 | 14 |
| α-helix | 103-107 | 5 | |
| α-helix | 110-127 | 18 | |
| β-strand | 134-140 | 7 | 14 |
| α-helix | 145-149 | 5 | |
| α-helix | 150-160 | 11 | |
| β-strand | 165-172 | 8 | 14 |
| α-helix | 183-197 | 15 | |
| β-strand | 200-205 | 6 | 14 |
| α-helix | 206-211 | 6 | |
| α-helix | 212-216 | 5 | |
| α-helix | 224-243 | 20 | |
| α-helix | 252-259 | 8 | |
| β-strand | 267-268 | 2 | 14 |
| β-strand | 269-273 | 5 | 17 |
| α-helix | 288-295 | 8 | |
| α-helix | 298-300 | 3 | |
| β-strand | 301 | 1 | 17 |
| α-helix | 307-309 | 3 | |
| β-strand | 312-321 | 10 | 17 |
| α-helix | 325-338 | 14 | |
| α-helix | 340-342 | 3 | |
| β-strand | 343 | 1 | 17 |
| β-strand | 351-356 | 6 | 17 |
| α-helix | 359-360 | 2 | |
| β-strand | 373-381 | 9 | 17 |
| α-helix | 382-384 | 3 | |
| α-helix | 385-399 | 15 | |
| α-helix | 406-409 | 4 | |
| α-helix | 415-437 | 23 | |
Chain E: 1 helix, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 7-14 | 8 | 3 |
| β-strand | 17-25 | 9 | 3 |
| α-helix | 47-142 | 96 | |
Chain F: 15 helices, 20 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 2-6 | 5 | 18 |
| α-helix | 12-23 | 12 | |
| β-strand | 27-30 | 4 | 18 |
| β-strand | 39-41 | 3 | 18 |
| α-helix | 49-51 | 3 | |
| β-strand | 61-62 | 2 | 18 |
| α-helix | 69-72 | 4 | |
| α-helix | 74-83 | 10 | |
| β-strand | 97-100 | 4 | 19 |
| α-helix | 128-140 | 13 | |
| β-strand | 147-150 | 4 | 19 |
| β-strand | 161-163 | 3 | 19 |
| α-helix | 166-174 | 9 | |
| β-strand | 180-184 | 5 | 19 |
| β-strand | 189 | 1 | 20 |
| β-strand | 192 | 1 | 21 |
| β-strand | 197 | 1 | 21 |
| β-strand | 199-207 | 9 | 22 |
| β-strand | 213-216 | 4 | 22 |
| β-strand | 220-223 | 4 | 22 |
| α-helix | 243-249 | 7 | |
| α-helix | 258-260 | 3 | |
| β-strand | 261-263 | 3 | 22 |
| α-helix | 264-275 | 12 | |
| α-helix | 279 | 1 | |
| α-helix | 280-284 | 5 | |
| α-helix | 285-302 | 18 | |
| β-strand | 310-311 | 2 | 18 |
| β-strand | 313-321 | 9 | 22 |
| β-strand | 322 | 1 | 20 |
| β-strand | 327-333 | 7 | 22 |
| α-helix | 340-342 | 3 | |
| α-helix | 343-350 | 8 | |
| α-helix | 351-355 | 5 | |
| β-strand | 375-377 | 3 | 22 |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Tubulin alpha-1B chain | A, C | protein | 451 | Bos taurus | P81947 (AlphaFold model) |
| Tubulin beta-2B chain | B, D | protein | 445 | Bos taurus | Q6B856 (AlphaFold model) |
| Stathmin-4 | E | protein | 143 | Rattus norvegicus | P63043 (AlphaFold model) |
| Tubulin-Tyrosine Ligase | F | protein | 384 | Gallus gallus | A0A8V0Z8P0 (AlphaFold model) |
Sequence of entity 1 (A, C), FASTA
>5S5Q_1 Tubulin alpha-1B chain (chains A, C)
MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGK
HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLD
RIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTA
VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITA
SLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPAN
QMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPP
TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
AREDMAALEKDYEEVGVDSVEGEGEEEGEEY
Sequence of entity 2 (B, D), FASTA
>5S5Q_2 Tubulin beta-2B chain (chains B, D)
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYV
PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV
RKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVV
EPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCL
RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMM
AACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRG
LKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
EYQQYQDATADEQGEFEEEEGEDEA
Sequence of entity 3 (E), FASTA
>5S5Q_3 Stathmin-4 (chains E)
MADMEVIELNKCTSGQSFEVILKPPSFDGVPEFNASLPRRRDPSLEEIQKKLEAAEERRK
YQEAELLKHLAEKREHEREVIQKAIEENNNFIKMAKEKLAQKMESNKENREAHLAAMLER
LQEKDKHAEEVRKNKELKEEASR
Sequence of entity 4 (F), FASTA
>5S5Q_4 Tubulin-Tyrosine Ligase (chains F)
MYTFVVRDENSSVYAEVSRLLLATGQWKRLRKDNPRFNLMLGERNRLPFGRLGHEPGLVQ
LVNYYRGADKLCRKASLVKLIKTSPELSESCTWFPESYVIYPTNLKTPVAPAQNGIRHLI
NNTRTDEREVFLAAYNRRREGREGNVWIAKSSAGAKGEGILISSEASELLDFIDEQGQVH
VIQKYLEKPLLLEPGHRKFDIRSWVLVDHLYNIYLYREGVLRTSSEPYNSANFQDKTCHL
TNHCIQKEYSKNYGRYEEGNEMFFEEFNQYLMDALNTTLENSILLQIKHIIRSCLMCIEP
AISTKHLHYQSFQLFGFDFMVDEELKVWLIEVNGAPACAQKLYAELCQGIVDVAISSVFP
LADTGQKTSQPTSIFIKLHHHHHH
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| GTP | Guanosine-5'-triphosphate | C10 H16 N5 O14 P3 | 2 |
| MG | Magnesium ion | Mg | 5 |
| CA | Calcium ion | Ca | 4 |
| GDP | Guanosine-5'-diphosphate | C10 H15 N5 O11 P2 | 2 |
| HR5 | ~{N}-(cyclobutylmethyl)-1,5-dimethyl-pyrazole-4-carboxamide | C11 H17 N3 O | 1 |
| ACP | Phosphomethylphosphonic acid adenylate ester | C11 H18 N5 O12 P3 | 1 |
Water and common crystallization additives (MES) are not listed.
Primary citation
Comprehensive Analysis of Binding Sites in Tubulin. Muhlethaler, T., Gioia, D., Prota, A.E. et al. Angew Chem Int Ed Engl (2021) 60:13331-13342. DOI 10.1002/anie.202100273 · PubMed
Other PDB entries of the same protein (UniProt P81947 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 6S8K 1.52 Å, Structure, Thermodynamics, and Kinetics of Plinabulin Binding to two Tubulin Isotypes
- 8QL2 1.7 Å, Ultrafast structural transitions in an azobenzene photoswitch at near-atomic resolution:…
- 6ZWB 1.75 Å, Z-SBTub3 photoswitch bound to tubulin-DARPin D1 complex
- 4I4T 1.8 Å, Crystal structure of tubulin-RB3-TTL-Zampanolide complex
- 5IYZ 1.8 Å, Tubulin-MMAE complex
- 5NQU 1.8 Å, Tubulin Darpin cryo structure
- 7YZ3 1.8 Å, Molecular snapshots of drug release from tubulin: Apo state
- 8QEA 1.8 Å, Ultrafast structural transitions in an azobenzene photoswitch at near-atomic resolution:…
- 8QL3 1.8 Å, Ultrafast structural transitions in an azobenzene photoswitch at near-atomic resolution:…
- 8QL4 1.8 Å, Ultrafast structural transitions in an azobenzene photoswitch at near-atomic resolution:…
- 8QL5 1.8 Å, Ultrafast structural transitions in an azobenzene photoswitch at near-atomic resolution:…
- 8QL6 1.8 Å, Ultrafast structural transitions in an azobenzene photoswitch at near-atomic resolution:…
Browse structure collections
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