5T94: Kap60

Crystal structure of Kap60 bound to yeast RCC1 (Prp20). Determined by X-ray diffraction at 2.63 Å resolution. Released 13 Sept 2017.

Method
X-ray diffraction
Resolution
2.63 Å
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Chains
2
Atoms
6,921
Mol. weight
113.58 kDa
Released
13 Sept 2017

Explore 5T94 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5T94 contains 43 α-helices and 33 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 33 β-strands

ElementResiduesLengthSheet
α-helix33-353
α-helix37-404
β-strand47-5371
α-helix65-673
β-strand69-7571
β-strand86-9162
β-strand95-10062
β-strand105-10952
α-helix145-1484
β-strand151-15222
α-helix153-1542
α-helix155-1573
α-helix159-1624
β-strand167-17263
β-strand176-18163
β-strand186-19053
β-strand192-19324
β-strand198-20034
β-strand20215
β-strand20615
β-strand209-21573
α-helix216-2172
β-strand223-22866
β-strand232-23766
β-strand242-24656
β-strand269-27026
β-strand276-28167
β-strand285-29067
β-strand295-30067
β-strand317-32377
β-strand332-33768
β-strand341-34668
β-strand351-35668
β-strand374-37528
β-strand381-38998
α-helix394-3952
β-strand396-40169
β-strand405-41069
β-strand415-41959
β-strand435-44069
β-strand449-45681
β-strand460-46781
α-helix468-4692
α-helix472-4809
Chain B: 32 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix89-968
α-helix101-11515
α-helix123-1286
α-helix132-1376
α-helix145-15915
α-helix163-1719
α-helix175-18410
α-helix187-20317
α-helix205-2139
α-helix217-2226
α-helix223-2253
α-helix229-24214
α-helix252-2554
α-helix256-2583
α-helix259-2657
α-helix271-28414
α-helix289-2979
α-helix301-3066
α-helix313-32614
α-helix331-3399
α-helix342-3498
α-helix355-36814
α-helix373-3819
α-helix385-39410
α-helix397-41115
α-helix412-4143
α-helix419-4268
α-helix430-43910
α-helix442-46221
α-helix475-4795
α-helix483-4875
α-helix495-50814

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Guanine nucleotide exchange factor SRM1Aprotein482Saccharomyces cerevisiae (strain ATCC 204508 / S288c)P21827 (AlphaFold model)
Importin subunit alphaBprotein542Saccharomyces cerevisiae (strain ATCC 204508 / S288c)Q02821 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>5T94_1 Guanine nucleotide exchange factor SRM1 (chains A)
MVKRTVATNGDASGAHRAKKMSKTHASHIINAQEDYKHMYLSVQPLDIFCWGTGSMCELG
LGPLAKNKEVKRPRLNPFLPRDEAKIISFAVGGMHTLALDEESNVWSWGCNDVGALGRDT
SNAKEQLKDMDADDSSDDEDGDLNELESTPAKIPRESFPPLAEGHKVVQLAATDNMSCAL
FSNGEVYAWGTFRCNEGILGFYQDKIKIQKTPWKVPTFSKYNIVQLAPGKDHILFLDEEG
MVFAWGNGQQNQLGRKVMERFRLKTLDPRPFGLRHVKYIASGENHCFALTKDNKLVSWGL
NQFGQCGVSEDVEDGALVTKPKRLALPDNVVIRSIAAGEHHSLILSQDGDLYSCGRLDMF
EVGIPKDNLPEYTYKDVHGKARAVPLPTKLNNVPKFKSVAAGSHHSVAVAQNGIAYSWGF
GETYAVGLGPFEDDTEVPTRIKNTATQDHNIILVGCGGQFSVSGGVKLSDEDAEKRADEM
DD
Sequence of entity 2 (B), FASTA
>5T94_2 Importin subunit alpha (chains B)
MDNGTDSSTSKFVPEYRRTNFKNKGRFSADELRRRRDTQQVELRKAKRDEALAKRRNFIP
PTDGADSDEEDESSVSADQQFYSQLQQELPQMTQQLNSDDMQEQLSATVKFRQILSREHR
PPIDVVIQAGVVPRLVEFMRENQPEMLQLEAAWALTNIASGTSAQTKVVVDADAVPLFIQ
LLYTGSVEVKEQAIWALGNVAGDSTDYRDYVLQCNAMEPILGLFNSNKPSLIRTATWTLS
NLCRGKKPQPDWSVVSQALPTLAKLIYSMDTETLVDACWAISYLSDGPQEAIQAVIDVRI
PKRLVELLSHESTLVQTPALRAVGNIVTGNDLQTQVVINAGVLPALRLLLSSPKENIKKE
ACWTISNITAGNTEQIQAVIDANLIPPLVKLLEVAEYKTKKEACWAISNASSGGLQRPDI
IRYLVSQGCIKPLCDLLEIADNRIIEVTLDALENILKMGEADKEARGLNINENADFIEKA
GGMEKIFNCQQNENDKIYEKAYKIIETYFGEEEDAVDETMAPQNAGNTFGFGSNVNQQFN
FN

Primary citation

Three-dimensional context rather than NLS amino acid sequence determines importin alpha subtype specificity for RCC1. Sankhala, R.S., Lokareddy, R.K., Begum, S. et al. Nat Commun (2017) 8:979-979. DOI 10.1038/s41467-017-01057-7 · PubMed

Other PDB entries of the same protein (UniProt P21827 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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