Crystal structure of USP35 C450S in complex with ubiquitin. Determined by X-ray diffraction at 1.84 Å resolution. Released 9 May 2018.
Explore 5TXK in 3D Show helices and sheets RCSB PDB PDBe
5TXK contains 16 α-helices and 29 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 442-443 | 2 | 1 |
| α-helix | 450-461 | 12 | |
| α-helix | 463-470 | 8 | |
| α-helix | 479-493 | 15 | |
| β-strand | 498-499 | 2 | 1 |
| α-helix | 502-507 | 6 | |
| α-helix | 514 | 1 | |
| β-strand | 519 | 1 | 2 |
| α-helix | 521-549 | 29 | |
| α-helix | 565-570 | 6 | |
| β-strand | 572-580 | 9 | 3 |
| β-strand | 586-593 | 8 | 3 |
| β-strand | 596-598 | 3 | 4 |
| α-helix | 599-602 | 4 | |
| β-strand | 760 | 1 | 5 |
| α-helix | 761-768 | 8 | |
| β-strand | 772-774 | 3 | 3 |
| α-helix | 776-778 | 3 | |
| β-strand | 780-782 | 3 | 6 |
| β-strand | 787-789 | 3 | 6 |
| β-strand | 791-799 | 9 | 3 |
| β-strand | 803-808 | 6 | 4 |
| β-strand | 811-814 | 4 | 7 |
| β-strand | 819-822 | 4 | 7 |
| β-strand | 828 | 1 | 5 |
| β-strand | 832-836 | 5 | 4 |
| α-helix | 838-840 | 3 | |
| β-strand | 842-854 | 13 | 4 |
| β-strand | 862-867 | 6 | 4 |
| β-strand | 891-894 | 4 | 4 |
| β-strand | 897-900 | 4 | 4 |
| α-helix | 903-912 | 10 | |
| β-strand | 916-925 | 10 | 4 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 2-6 | 5 | 8 |
| β-strand | 12-16 | 5 | 8 |
| β-strand | 22 | 1 | 9 |
| α-helix | 23-34 | 12 | |
| α-helix | 38-40 | 3 | |
| β-strand | 42-45 | 4 | 8 |
| β-strand | 48-49 | 2 | 8 |
| α-helix | 50-51 | 2 | |
| β-strand | 55 | 1 | 9 |
| α-helix | 57-59 | 3 | |
| β-strand | 66-70 | 5 | 8 |
| β-strand | 75 | 1 | 2 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Ubiquitin carboxyl-terminal hydrolase 35,Ubiquitin carboxyl-terminal hydrolase 35 | A | protein | 372 | Homo sapiens | Q9P2H5 (AlphaFold model) |
| Polyubiquitin-B | B | protein | 76 | Homo sapiens | P0CG47 (AlphaFold model) |
>5TXK_1 Ubiquitin carboxyl-terminal hydrolase 35,Ubiquitin carboxyl-terminal hydrolase 35 (chains A) SELAGFYPRLMAKSDTGKIGLINLGNTSYVNSILQALFMASDFRHCVLRLTENNSQPLMT KLQWLFGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRI CQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEAFTDLSLAFPP PGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCASLQDAEKVVELSQGPCYLILTLLRFS FDLRTMRRRKILDDVSIPLLLRLPLAGGRGQAYDLCSVVVHSGVSSESGHYYCYAREGAA RPAASLGTADRPEPENQWYLFNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPE AELGSSRVRTEP
>5TXK_2 Polyubiquitin-B (chains B) MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG
| ID | Name | Formula | Copies |
|---|---|---|---|
| ZN | Zinc ion | Zn | 1 |
Water and common crystallization additives (EDO, SO4) are not listed.
Expansion of DUB functionality generated by alternative isoforms - USP35, a case study. Leznicki, P., Natarajan, J., Bader, G. et al. J Cell Sci (2018) 131. DOI 10.1242/jcs.212753 · PubMed
Other PDB entries of the same protein (UniProt Q9P2H5 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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