5W1S: Escherichia coli RNA polymerase and TraR complex
X-ray crystal structure of Escherichia coli RNA polymerase and TraR complex. Determined by X-ray diffraction at 3.81 Å resolution. Released 14 Jun 2017.
- Method
- X-ray diffraction
- Resolution
- 3.81 Å
- Organism
- Escherichia coli (strain K12)
- Chains
- 14
- Atoms
- 56,825
- Mol. weight
- 938.56 kDa
- Ligands
- ZN, MG
- Released
- 14 Jun 2017
Explore 5W1S in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
5W1S contains 336 α-helices and 388 β-strands across 14 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 12 helices, 22 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 13-20 | 8 | 1 |
| β-strand | 23-28 | 6 | 1 |
| β-strand | 31 | 1 | 2 |
| α-helix | 35-48 | 14 | |
| β-strand | 52-61 | 10 | 3 |
| β-strand | 74 | 1 | 4 |
| α-helix | 78-85 | 8 | |
| β-strand | 90-91 | 2 | 5 |
| β-strand | 98-105 | 8 | 3 |
| β-strand | 109-111 | 3 | 6 |
| α-helix | 112-114 | 3 | |
| β-strand | 115 | 1 | 3 |
| β-strand | 122-123 | 2 | 5 |
| β-strand | 129-132 | 4 | 6 |
| β-strand | 133 | 1 | 4 |
| β-strand | 139-150 | 12 | 3 |
| β-strand | 151-153 | 3 | 7 |
| α-helix | 155-158 | 4 | |
| β-strand | 170-172 | 3 | 3 |
| β-strand | 175-177 | 3 | 7 |
| β-strand | 180-186 | 7 | 1 |
| β-strand | 189 | 1 | 2 |
| β-strand | 199 | 1 | 2 |
| β-strand | 202-207 | 6 | 1 |
| α-helix | 213-232 | 20 | |
| α-helix | 251-254 | 4 | |
| β-strand | 256 | 1 | 8 |
| α-helix | 257-260 | 4 | |
| α-helix | 264-272 | 9 | |
| β-strand | 277 | 1 | 8 |
| α-helix | 278-281 | 4 | |
| α-helix | 287-290 | 4 | |
| α-helix | 297-301 | 5 | |
| α-helix | 303-310 | 8 | |
Chain B: 4 helices, 13 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 14-17 | 4 | 9 |
| β-strand | 23-31 | 9 | 9 |
| α-helix | 32 | 1 | |
| α-helix | 35-48 | 14 | |
| β-strand | 52-60 | 9 | 10 |
| α-helix | 78-86 | 9 | |
| β-strand | 90-92 | 3 | 10 |
| β-strand | 99-101 | 3 | 10 |
| β-strand | 110 | 1 | 11 |
| β-strand | 121-123 | 3 | 10 |
| β-strand | 131 | 1 | 11 |
| β-strand | 143-150 | 8 | 10 |
| β-strand | 152-153 | 2 | 12 |
| β-strand | 175-176 | 2 | 12 |
| β-strand | 180-190 | 11 | 9 |
| β-strand | 198-207 | 10 | 9 |
| α-helix | 213-226 | 14 | |
Chain C: 57 helices, 98 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 13-14 | 2 | 13 |
| α-helix | 19-20 | 2 | |
| α-helix | 29-38 | 10 | |
| α-helix | 49-56 | 8 | |
| α-helix | 57 | 1 | |
| β-strand | 59-60 | 2 | 14 |
| β-strand | 66-75 | 10 | 14 |
| α-helix | 76-78 | 3 | |
| α-helix | 82-88 | 7 | |
| β-strand | 93-105 | 13 | 14 |
| β-strand | 113-117 | 5 | 14 |
| β-strand | 122-128 | 7 | 14 |
| β-strand | 136-137 | 2 | 15 |
| β-strand | 142-145 | 4 | 15 |
| β-strand | 147-151 | 5 | 16 |
| α-helix | 152 | 1 | |
| β-strand | 154-158 | 5 | 17 |
| β-strand | 173-177 | 5 | 17 |
| β-strand | 180 | 1 | 18 |
| β-strand | 184-187 | 4 | 17 |
| β-strand | 195-198 | 4 | 17 |
| β-strand | 204-205 | 2 | 17 |
| α-helix | 208-212 | 5 | |
| α-helix | 217-224 | 8 | |
| β-strand | 228-231 | 4 | 19 |
| β-strand | 238-240 | 3 | 19 |
| β-strand | 250 | 1 | 20 |
| β-strand | 255-257 | 3 | 21 |
| β-strand | 260-263 | 4 | 21 |
| β-strand | 268 | 1 | 20 |
| α-helix | 271-280 | 10 | |
| β-strand | 284-286 | 3 | 19 |
| α-helix | 291-293 | 3 | |
| α-helix | 295 | 1 | |
| β-strand | 296-297 | 2 | 19 |
| β-strand | 301-302 | 2 | 22 |
| β-strand | 309-311 | 3 | 22 |
| β-strand | 315-316 | 2 | 19 |
| α-helix | 319-326 | 8 | |
| β-strand | 332-335 | 4 | 19 |
| α-helix | 346-351 | 6 | |
| α-helix | 359-370 | 12 | |
| α-helix | 378-389 | 12 | |
| β-strand | 396 | 1 | 18 |
| α-helix | 398-408 | 11 | |
| α-helix | 422-437 | 16 | |
| β-strand | 451-454 | 4 | 16 |
| α-helix | 456-481 | 26 | |
| α-helix | 496-507 | 12 | |
| β-strand | 509 | 1 | 15 |
| β-strand | 512-514 | 3 | 15 |
| α-helix | 520-527 | 8 | |
| β-strand | 529 | 1 | 23 |
| β-strand | 530-531 | 2 | 16 |
| α-helix | 545-548 | 4 | |
| α-helix | 550-551 | 2 | |
| α-helix | 552-554 | 3 | |
| β-strand | 558 | 1 | 24 |
| β-strand | 574 | 1 | 23 |
| β-strand | 575 | 1 | 24 |
| β-strand | 576 | 1 | 25 |
| β-strand | 580-581 | 2 | 26 |
| β-strand | 587-595 | 9 | 26 |
| β-strand | 598-607 | 10 | 26 |
| α-helix | 608-613 | 6 | |
| β-strand | 616 | 1 | 26 |
| β-strand | 623-624 | 2 | 26 |
| β-strand | 628-629 | 2 | 26 |
| β-strand | 633-637 | 5 | 26 |
| β-strand | 642-646 | 5 | 26 |
| β-strand | 652-654 | 3 | 26 |
| β-strand | 662 | 1 | 25 |
| α-helix | 663-666 | 4 | |
| α-helix | 671-673 | 3 | |
| β-strand | 674 | 1 | 27 |
| α-helix | 676-685 | 10 | |
| α-helix | 686-688 | 3 | |
| β-strand | 689 | 1 | 28 |
| α-helix | 690-692 | 3 | |
| β-strand | 700-701 | 2 | 13 |
| α-helix | 705-711 | 7 | |
| β-strand | 716-717 | 2 | 29 |
| β-strand | 722-727 | 6 | 30 |
| β-strand | 731-736 | 6 | 30 |
| β-strand | 748-752 | 5 | 30 |
| α-helix | 753-754 | 2 | |
| β-strand | 757-758 | 2 | 31 |
| β-strand | 763 | 1 | 32 |
| β-strand | 764-765 | 2 | 31 |
| β-strand | 768-769 | 2 | 29 |
| β-strand | 776-777 | 2 | 30 |
| β-strand | 782-785 | 4 | 29 |
| β-strand | 789-790 | 2 | 33 |
| β-strand | 793-794 | 2 | 33 |
| β-strand | 798-804 | 7 | 34 |
| β-strand | 816-819 | 4 | 34 |
| α-helix | 820-824 | 5 | |
| β-strand | 830-841 | 12 | 32 |
| β-strand | 842-843 | 2 | 35 |
| β-strand | 846-847 | 2 | 35 |
| β-strand | 849 | 1 | 36 |
| α-helix | 859-862 | 4 | |
| β-strand | 865 | 1 | 37 |
| β-strand | 870 | 1 | 38 |
| β-strand | 871 | 1 | 37 |
| α-helix | 872 | 1 | |
| β-strand | 876-877 | 2 | 32 |
| β-strand | 882-884 | 3 | 38 |
| β-strand | 887-889 | 3 | 36 |
| α-helix | 897-906 | 10 | |
| β-strand | 913-915 | 3 | 36 |
| β-strand | 918-919 | 2 | 38 |
| α-helix | 920-921 | 2 | |
| β-strand | 926-935 | 10 | 32 |
| α-helix | 940-941 | 2 | |
| α-helix | 943-979 | 37 | |
| α-helix | 986-989 | 4 | |
| α-helix | 994-996 | 3 | |
| α-helix | 1006-1037 | 32 | |
| α-helix | 1039-1041 | 3 | |
| β-strand | 1046-1058 | 13 | 32 |
| β-strand | 1065-1068 | 4 | 34 |
| β-strand | 1073-1080 | 8 | 34 |
| α-helix | 1082-1084 | 3 | |
| β-strand | 1087 | 1 | 39 |
| β-strand | 1093 | 1 | 39 |
| β-strand | 1096-1098 | 3 | 34 |
| α-helix | 1100-1102 | 3 | |
| β-strand | 1108 | 1 | 27 |
| α-helix | 1110-1133 | 24 | |
| α-helix | 1138-1148 | 11 | |
| α-helix | 1167-1176 | 10 | |
| β-strand | 1182-1183 | 2 | 13 |
| α-helix | 1192-1201 | 10 | |
| β-strand | 1209-1210 | 2 | 40 |
| β-strand | 1213 | 1 | 39 |
| α-helix | 1219 | 1 | |
| β-strand | 1220 | 1 | 39 |
| α-helix | 1221 | 1 | |
| β-strand | 1225-1226 | 2 | 40 |
| β-strand | 1227-1234 | 8 | 34 |
| β-strand | 1235 | 1 | 28 |
| α-helix | 1239-1242 | 4 | |
| β-strand | 1244-1246 | 3 | 41 |
| β-strand | 1250-1251 | 2 | 42 |
| β-strand | 1252 | 1 | 43 |
| β-strand | 1258-1259 | 2 | 42 |
| α-helix | 1265-1268 | 4 | |
| β-strand | 1269 | 1 | 44 |
| α-helix | 1272-1280 | 9 | |
| α-helix | 1284-1290 | 7 | |
| α-helix | 1298-1310 | 13 | |
| α-helix | 1321-1331 | 11 | |
| β-strand | 1335 | 1 | 45 |
| β-strand | 1338-1339 | 2 | 46 |
Chain D: 61 helices, 61 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 20-21 | 2 | 46 |
| β-strand | 24 | 1 | 45 |
| α-helix | 27-33 | 7 | |
| β-strand | 34-37 | 4 | 47 |
| β-strand | 40 | 1 | 48 |
| β-strand | 44 | 1 | 49 |
| α-helix | 50 | 1 | |
| β-strand | 51 | 1 | 49 |
| α-helix | 52 | 1 | |
| β-strand | 55 | 1 | 48 |
| β-strand | 84 | 1 | 50 |
| β-strand | 91 | 1 | 50 |
| α-helix | 97-100 | 4 | |
| β-strand | 102-112 | 11 | 47 |
| α-helix | 114-118 | 5 | |
| α-helix | 123-127 | 5 | |
| α-helix | 132-139 | 8 | |
| β-strand | 144-147 | 4 | 51 |
| β-strand | 159-160 | 2 | 51 |
| α-helix | 162-171 | 10 | |
| β-strand | 177-179 | 3 | 51 |
| α-helix | 182-191 | 10 | |
| α-helix | 194-207 | 14 | |
| α-helix | 211-230 | 20 | |
| α-helix | 234-237 | 4 | |
| β-strand | 238-244 | 7 | 47 |
| α-helix | 247-249 | 3 | |
| β-strand | 254 | 1 | 52 |
| α-helix | 256-258 | 3 | |
| β-strand | 260 | 1 | 52 |
| β-strand | 261-262 | 2 | 53 |
| α-helix | 264-284 | 21 | |
| α-helix | 289-308 | 20 | |
| α-helix | 315 | 1 | |
| β-strand | 316 | 1 | 54 |
| α-helix | 317 | 1 | |
| β-strand | 323 | 1 | 54 |
| α-helix | 324-325 | 2 | |
| α-helix | 327-331 | 5 | |
| β-strand | 346 | 1 | 44 |
| β-strand | 350-354 | 5 | 41 |
| β-strand | 355-356 | 2 | 55 |
| β-strand | 365-369 | 5 | 55 |
| α-helix | 370-376 | 7 | |
| α-helix | 378-387 | 10 | |
| α-helix | 394-403 | 10 | |
| α-helix | 406-416 | 11 | |
| β-strand | 421-425 | 5 | 41 |
| α-helix | 431-433 | 3 | |
| β-strand | 434-437 | 4 | 41 |
| β-strand | 438-442 | 5 | 55 |
| β-strand | 447-448 | 2 | 55 |
| α-helix | 454-457 | 4 | |
| β-strand | 465-469 | 5 | 41 |
| α-helix | 474-482 | 9 | |
| β-strand | 485 | 1 | 41 |
| α-helix | 486-488 | 3 | |
| β-strand | 491 | 1 | 56 |
| β-strand | 498 | 1 | 56 |
| α-helix | 505-513 | 9 | |
| β-strand | 526-527 | 2 | 57 |
| α-helix | 530-538 | 9 | |
| β-strand | 547-556 | 10 | 57 |
| β-strand | 558 | 1 | 58 |
| β-strand | 562 | 1 | 58 |
| β-strand | 564-573 | 10 | 57 |
| α-helix | 574-580 | 7 | |
| α-helix | 589-592 | 4 | |
| α-helix | 598-612 | 15 | |
| α-helix | 614-635 | 22 | |
| α-helix | 641-643 | 3 | |
| α-helix | 645-647 | 3 | |
| α-helix | 650-669 | 20 | |
| α-helix | 675-703 | 29 | |
| β-strand | 706 | 1 | 59 |
| β-strand | 715 | 1 | 59 |
| α-helix | 721-728 | 8 | |
| α-helix | 734-740 | 7 | |
| β-strand | 748 | 1 | 60 |
| β-strand | 754 | 1 | 60 |
| α-helix | 766-767 | 2 | |
| α-helix | 769-803 | 35 | |
| β-strand | 809 | 1 | 61 |
| β-strand | 820-822 | 3 | 62 |
| α-helix | 835-839 | 5 | |
| β-strand | 843 | 1 | 63 |
| β-strand | 844 | 1 | 62 |
| α-helix | 847 | 1 | |
| β-strand | 848-849 | 2 | 64 |
| β-strand | 856-858 | 3 | 64 |
| β-strand | 863 | 1 | 63 |
| α-helix | 866-874 | 9 | |
| β-strand | 880-882 | 3 | 62 |
| β-strand | 894 | 1 | 61 |
| α-helix | 896-899 | 4 | |
| α-helix | 908-910 | 3 | |
| α-helix | 915-924 | 10 | |
| α-helix | 925-929 | 5 | |
| α-helix | 1138-1145 | 8 | |
| α-helix | 1148-1150 | 3 | |
| β-strand | 1156 | 1 | 65 |
| β-strand | 1161-1166 | 6 | 66 |
| β-strand | 1173-1178 | 6 | 66 |
| β-strand | 1187-1190 | 4 | 66 |
| β-strand | 1196 | 1 | 67 |
| β-strand | 1203-1204 | 2 | 66 |
| β-strand | 1209 | 1 | 65 |
| β-strand | 1211 | 1 | 67 |
| α-helix | 1217-1219 | 3 | |
| α-helix | 1220-1224 | 5 | |
| α-helix | 1226-1243 | 18 | |
| α-helix | 1250-1260 | 11 | |
| β-strand | 1263-1265 | 3 | 68 |
| β-strand | 1277-1279 | 3 | 68 |
| α-helix | 1280-1291 | 12 | |
| β-strand | 1304-1305 | 2 | 68 |
| α-helix | 1309-1314 | 6 | |
| α-helix | 1319-1325 | 7 | |
| α-helix | 1328-1338 | 11 | |
| β-strand | 1341-1342 | 2 | 46 |
| α-helix | 1347-1352 | 6 | |
| α-helix | 1360-1370 | 11 | |
Chain E: 6 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-12 | 6 | |
| α-helix | 18-31 | 14 | |
| α-helix | 46-55 | 10 | |
| α-helix | 61-78 | 18 | |
| α-helix | 80-83 | 4 | |
| α-helix | 87-89 | 3 | |
Chain F: 27 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 97-106 | 10 | |
| α-helix | 109-112 | 4 | |
| α-helix | 115-135 | 21 | |
| α-helix | 138-152 | 15 | |
| α-helix | 158-161 | 4 | |
| β-strand | 162-165 | 4 | 69 |
| α-helix | 214-233 | 20 | |
| α-helix | 245-256 | 12 | |
| β-strand | 259-261 | 3 | 69 |
| α-helix | 263-288 | 26 | |
| α-helix | 289-293 | 5 | |
| α-helix | 328-331 | 4 | |
| α-helix | 334-339 | 6 | |
| α-helix | 342-350 | 9 | |
| α-helix | 355-382 | 28 | |
| α-helix | 384-391 | 8 | |
| α-helix | 401-418 | 18 | |
| α-helix | 421-423 | 3 | |
| α-helix | 427-446 | 20 | |
| α-helix | 454-473 | 20 | |
| α-helix | 483-486 | 4 | |
| α-helix | 493-501 | 9 | |
| α-helix | 503-504 | 2 | |
| β-strand | 505-506 | 2 | 53 |
| β-strand | 510 | 1 | 70 |
| β-strand | 518 | 1 | 70 |
| α-helix | 519-522 | 4 | |
| β-strand | 524 | 1 | 43 |
| α-helix | 531-549 | 19 | |
| α-helix | 553-562 | 10 | |
| α-helix | 573-578 | 6 | |
| α-helix | 585-597 | 13 | |
| α-helix | 604-608 | 5 | |
Chain G: 7 helices, 15 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 13-18 | 6 | 71 |
| β-strand | 24-31 | 8 | 71 |
| α-helix | 32 | 1 | |
| α-helix | 35-49 | 15 | |
| β-strand | 53-61 | 9 | 72 |
| α-helix | 78-86 | 9 | |
| β-strand | 90-92 | 3 | 73 |
| β-strand | 97-105 | 9 | 72 |
| β-strand | 108-110 | 3 | 74 |
| α-helix | 112-114 | 3 | |
| β-strand | 115 | 1 | 72 |
| α-helix | 116-118 | 3 | |
| β-strand | 121-123 | 3 | 73 |
| β-strand | 131-133 | 3 | 74 |
| β-strand | 139-149 | 11 | 72 |
| β-strand | 151-153 | 3 | 75 |
| α-helix | 156-158 | 3 | |
| β-strand | 170-172 | 3 | 72 |
| β-strand | 175-177 | 3 | 75 |
| β-strand | 180-190 | 11 | 71 |
| β-strand | 198-207 | 10 | 71 |
| α-helix | 213-227 | 15 | |
Chain H: 4 helices, 15 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 15-17 | 3 | 76 |
| β-strand | 23-31 | 9 | 76 |
| α-helix | 32 | 1 | |
| α-helix | 35-48 | 14 | |
| β-strand | 53-60 | 8 | 77 |
| α-helix | 78-86 | 9 | |
| β-strand | 90-92 | 3 | 78 |
| β-strand | 99-103 | 5 | 77 |
| β-strand | 110-111 | 2 | 79 |
| β-strand | 115 | 1 | 77 |
| β-strand | 121-123 | 3 | 78 |
| β-strand | 129-131 | 3 | 79 |
| β-strand | 141-149 | 9 | 77 |
| β-strand | 152-153 | 2 | 80 |
| β-strand | 172 | 1 | 77 |
| β-strand | 175-176 | 2 | 80 |
| β-strand | 180-192 | 13 | 76 |
| β-strand | 195-207 | 13 | 76 |
| α-helix | 213-227 | 15 | |
6 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| DNA-directed RNA polymerase subunit alpha | A, B, G, H | protein | 329 | Escherichia coli (strain K12) | P0A7Z4 (AlphaFold model) |
| DNA-directed RNA polymerase subunit beta | C, I | protein | 1342 | Escherichia coli (strain K12) | P0A8V2 (AlphaFold model) |
| DNA-directed RNA polymerase subunit beta' | D, J | protein | 1407 | Escherichia coli (strain K12) | P0A8T7 (AlphaFold model) |
| DNA-directed RNA polymerase subunit omega | E, K | protein | 91 | Escherichia coli (strain K12) | P0A800 (AlphaFold model) |
| RNA polymerase sigma factor RpoD | F, L | protein | 613 | Escherichia coli (strain K12) | P00579 |
| Protein TraR | M, N | protein | 79 | Escherichia coli (strain K12) | P41065 |
Sequence of entity 1 (A, B, G, H), FASTA
>5W1S_1 DNA-directed RNA polymerase subunit alpha (chains A, B, G, H)
MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE
IDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTLNKSGIGPVTAADITHDGD
VEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIHSEEDERPIGRLLVDACYSPV
ERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIRRAATILAEQLEAFVDLRDVRQP
EVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSL
TEIKDVLASRGLSLGMRLENWPPASIADE
Sequence of entity 2 (C, I), FASTA
>5W1S_2 DNA-directed RNA polymerase subunit beta (chains C, I)
MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQ
SYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQ
EVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYR
GSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQME
LVPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKD
YIDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRL
SALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLREEIEGSGIL
SKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLS
LGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTR
ERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVT
DEIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQV
VSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKR
GGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERG
DVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVS
RDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEK
LLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDL
SEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDE
LKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKIN
PIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAK
LREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLK
LGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLG
GKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
ESFNVLLKEIRSLGINIELEDE
Sequence of entity 3 (D, J), FASTA
>5W1S_3 DNA-directed RNA polymerase subunit beta' (chains D, J)
MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPERDGLFCAR
IFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERMGHIELASPTAHIWFLKSL
PSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQILTEEQYLDALEEFGDEFDAKM
GAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLTKRIKLLEAFVQSGNKPEWMILT
VLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQ
EAVDALLDNGRRGRAITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPY
LRLHQCGLPKKMALELFKPFIYGKLELRGLATTIKAAKKMVEREEAVVWDILDEVIREHP
VLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNADFDGDQMAVHVPLTLEAQLEA
RALMMSTNNILSPANGEPIIVPSQDVVLGLYYMTRDCVNAKGEGMVLTGPKEAERLYRSG
LASLHARVKVRITEYEKDANGELVAKTSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKA
ISKMLNTCYRILGLKPTVIFADQIMYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAE
VAEIQEQFQSGLVTAGERYNKVIDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFN
SIYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGAR
KGLADTALKTANSGYLTRRLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVL
GRVTAEDVLKPGTADILVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYG
RDLARGHIINKGEAIGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKL
SNVKSVVNSSGKLVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANW
DPHTMPVITEVSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKI
VDAQGNDVLIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPR
VADLFEARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFE
GERVERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQM
LRKATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEAPA
APQVTAEDASASLAELLNAGLGGSDNE
Sequence of entity 4 (E, K), FASTA
>5W1S_4 DNA-directed RNA polymerase subunit omega (chains E, K)
MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDKTTVIALREIEEGLIN
NQILDVRERQEQQEQEAAELQAVTAIAEGRR
Sequence of entity 5 (F, L), FASTA
>5W1S_5 RNA polymerase sigma factor RpoD (chains F, L)
MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVMEEAP
DADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTREGEIDIA
KRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSDLITGFVDPNAEEDLAPTATH
VGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAREKFAELRAQYVVTRDTIKAKGR
SHATAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVMMDRVRTQERLIMKLCVEQCKMPKK
NFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVSEEVHRALQKLQQIEEETGLTIEQVKD
INRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFE
YRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREPTP
EELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATTESL
RAATHDVLAGLTAREAKVLRMRFGIDMNTDYTLEEVGKQFDVTRERIRQIEAKALRKLRH
PSRSEVLRSFLDD
Sequence of entity 6 (M, N), FASTA
>5W1S_6 Protein TraR (chains M, N)
MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPIPEARRKIFPGVTLCVE
CQAYQERQRKHYAHHHHHH
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ZN | Zinc ion | Zn | 6 |
| MG | Magnesium ion | Mg | 2 |
Primary citation
Allosteric Effector ppGpp Potentiates the Inhibition of Transcript Initiation by DksA. Molodtsov, V., Sineva, E., Zhang, L. et al. Mol Cell (2018) 69:828-839.e5. DOI 10.1016/j.molcel.2018.01.035 · PubMed
Other PDB entries of the same protein (UniProt P0A7Z4 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 3K4G 2.05 Å, Crystal structure of E. coli RNA polymerase alpha subunit C-terminal domain
- 8FVW 2.1 Å, CryoEM structure of E.coli transcription elongation complex bound to ppGpp
- 8FVR 2.42 Å, CryoEM structure of E.coli transcription elongation complex
- 9ZP4 2.42 Å, E. coli RNA polymerase elongation complex containing the unnatural dZ:PTP base pair in a…
- 8HKC 2.49 Å, Cryo-EM structure of E. coli RNAP sigma32 complex
- 1BDF 2.5 Å, Structure of escherichia coli RNA polymerase alpha subunit N-terminal domain
- 6XL5 2.5 Å, Cryo-EM structure of EcmrR-RNAP-promoter open complex (EcmrR-RPo)
- 6XL9 2.5 Å, Cryo-EM structure of EcmrR-RNAP-promoter initial transcribing complex with 3-nt RNA…
- 12EC 2.6 Å, Half-translocated RNA polymerase elemental paused elongation complex, swiveled (ePEC…
- 8F1J 2.6 Å, SigN RNA polymerase early-melted intermediate bound to mismatch DNA fragment dhsU36mm2…
- 8PIB 2.6 Å, autoinhibited RfaH bound to E. coli transcription complex paused at ops site (encounter…
- 9MSF 2.6 Å, de novo SigN RNA polymerase transcription initiation intermediate with post-catalytic…
Browse structure collections
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