6CFZ: Ask1
Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface. Determined by electron microscopy at 4.5 Å resolution. Released 2 May 2018.
- Method
- Electron microscopy
- Resolution
- 4.5 Å
- Organism
- Chaetomium thermophilum
- Chains
- 10
- Atoms
- 5,990
- Mol. weight
- 116.15 kDa
- Released
- 2 May 2018
Explore 6CFZ in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
6CFZ contains 23 α-helices and 0 β-strands across 10 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 14-42 | 29 | |
| α-helix | 43-47 | 5 | |
| α-helix | 48-76 | 29 | |
Chain B: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 19-65 | 47 | |
| α-helix | 66-70 | 5 | |
| α-helix | 71-81 | 11 | |
Chain C: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 26-91 | 66 | |
Chain D: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 50-114 | 65 | |
Chain E: 2 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-39 | 36 | |
| α-helix | 42-64 | 23 | |
Chain F: 2 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 19-56 | 38 | |
| α-helix | 60-73 | 14 | |
Chain G: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 23-76 | 54 | |
Chain H: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 56-103 | 48 | |
2 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Ask1 | A | protein | 77 | Chaetomium thermophilum | G0S8F9 (AlphaFold model) |
| Dad3 | B | protein | 67 | Chaetomium thermophilum | G0RY74 (AlphaFold model) |
| Dad2 | C | protein | 94 | Chaetomium thermophilum | G0RZB3 (AlphaFold model) |
| Duo1 | D | protein | 111 | Chaetomium thermophilum | G0SAN7 (AlphaFold model) |
| Dad4 | E | protein | 72 | Chaetomium thermophilum | G0S589 |
| Dad1,Dad1 | F | protein | 92 | Chaetomium thermophilum | G0RYE9 |
| Hsk3 | G | protein | 61 | Chaetomium thermophilum | |
| Dam1 | H | protein | 56 | Chaetomium thermophilum | G0S2K4 |
| Spc19 | I | protein | 151 | Chaetomium thermophilum | G0S0N0 |
| Spc34 | J | protein | 245 | Chaetomium thermophilum | G0S2A3 |
Sequence of entity 1 (A), FASTA
>6CFZ_1 Ask1 (chains A)
MTLTEELEKLEQQITLTLQEIDSNFAKAHRIVTTSILPLVEQYGEHSRAVWEATKFWKQF
FEASANVSLSGYEELVD
Sequence of entity 2 (B), FASTA
>6CFZ_2 Dad3 (chains B)
MLSPLEQEVLDEYERLSENMKKLAVLLDELASAPATEILDGLRELERKTSLVFTLLKASV
YSIVLQQ
Sequence of entity 3 (C), FASTA
>6CFZ_3 Dad2 (chains C)
MSPALLARVNEKKAELENLKELRDLSAAVAAQMEALEQKLSTLSSGTEAIATVLANWHNV
LRAISMASAKIPEPKEETEENTVPLPQTLVRIPT
Sequence of entity 4 (D), FASTA
>6CFZ_4 Duo1 (chains D)
MEAREAALRKELEGVRKINEVIEGMIGTLERAKGNMGTVSQTVTNATTLLNTWTRMLSQT
EHNQRLILNPEWKGATQDLLELEAEERRRQEEVERRAAEAERRREEARRKA
Sequence of entity 5 (E), FASTA
>6CFZ_5 Dad4 (chains E)
MESPHEHQQNLLLSRIITNVEKLNEAIMVMNKTLQEINIQNMNIELVAQMFKNYQSNVLF
HLEATDNLKDPA
Sequence of entity 6 (F), FASTA
>6CFZ_6 Dad1,Dad1 (chains F)
MSYFEQQRQALIEEIAMNFEHVLANINKLNRSLEAVIAVGNEFSSVEALWSQFENVMSWS
HPQFEKGAMTGWSHPQFEKRSAGSWSHPQFEK
Sequence of entity 7 (G), FASTA
>6CFZ_7 Hsk3 (chains G)
MAVKARQLAHLHSQLTQLSHNLATTENLMRMTAVQAEAMRGLGSWHAGLFMAASKVLGEE
S
Sequence of entity 8 (H), FASTA
>6CFZ_8 Dam1 (chains H)
MNAFEPAFAELADAVADLEANMMHFQLMHESLARFSESFASFLYGLNMNAFCVDFP
Sequence of entity 9 (I), FASTA
>6CFZ_9 Spc19 (chains I)
MSYADCVCSLRTSLAFLESSVATLDNGVQDFPRLCHVLRTVRHYELIPQTTLAAAEASLR
DEIGPFIQLLLDRAEKHLDRQARRIETLKARAELNAGRLSQYSGDGHNNGKFSGQGMDFR
KSRPLNGEAALRAKVVRQRKEALKYSVERLE
Sequence of entity 10 (J), FASTA
>6CFZ_10 Spc34 (chains J)
MSLLSAHLEQISISCQGIDSLPFPPPKIFTNALLSNPDITSLIRDTEAHERALFSVPPPP
PRQTTLTAEQQQQQKPSNRRQTVFNVTGGEIRTGGVGSASTARRNTAVAAVLGGDLHAQI
MRGTRARPGQQPGSGDIDMEVLLRGVEKLCAVYPLPGALERVPVIRQKWQAQSNTLAYYE
AKIAEQQEMLDRIAQERMMNDGDGDVEMEDVEEVGMTEEDLRREEEEVRELDKRKRDLQH
HHHHH
Primary citation
Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface. Jenni, S., Harrison, S.C. Science (2018) 360:552-558. DOI 10.1126/science.aar6436 · PubMed
Browse structure collections
About this viewer
MolViewer shows 6CFZ directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.