6GWC: Tubulin:iE5 alphaRep complex

Tubulin:iE5 alphaRep complex. Determined by X-ray diffraction at 2.6 Å resolution. Released 30 Jan 2019.

Method: X-ray diffraction
Resolution: 2.6 Å
Organisms: Ovis aries, synthetic construct
Chains: 3
Atoms: 8,478
Mol. weight: 127.01 kDa
Ligands: GTP, MG
Released: 30 Jan 2019

Explore 6GWC in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6GWC contains 75 α-helices and 33 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 28 helices, 16 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	1
α-helix	10-28	19
α-helix	48-51	4
β-strand	53-55	3	2
β-strand	61-63	3	2
β-strand	65-69	5	1
α-helix	73-79	7
α-helix	89-91	3
β-strand	92-94	3	1
α-helix	103-104	2
α-helix	105-109	5
α-helix	111-113	3
α-helix	115-126	12
β-strand	132-140	9	1
α-helix	144-160	17
β-strand	165-172	8	1
α-helix	173-174	2
α-helix	183-194	12
β-strand	200-205	6	1
α-helix	206-215	10
α-helix	224-243	20
α-helix	252-259	8
α-helix	268	1
β-strand	269-273	5	3
β-strand	277	1	4
α-helix	284-287	4
α-helix	288-294	7
α-helix	298-300	3
β-strand	301	1	3
α-helix	307-309	3
β-strand	312-321	10	3
α-helix	325-338	14
α-helix	342	1
β-strand	343	1	3
α-helix	344	1
β-strand	352-356	5	3
α-helix	359-362	4
β-strand	368	1	4
α-helix	369-370	2
β-strand	373-381	9	3
α-helix	382-384	3
α-helix	385-399	15
α-helix	405-409	5
α-helix	416-436	21

Chain B: 25 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	5
α-helix	10-27	18
β-strand	30	1	6
β-strand	36	1	6
α-helix	42-47	4
α-helix	49-51	3
β-strand	53-54	2	7
β-strand	62-63	2	7
β-strand	65-69	5	5
α-helix	74-79	6
α-helix	89-91	3
β-strand	92-94	3	5
α-helix	103-107	5
α-helix	110-127	18
β-strand	132-140	9	5
α-helix	145-149	5
α-helix	150-160	11
β-strand	165-172	8	5
α-helix	173	1
α-helix	183-197	15
β-strand	200-205	6	5
α-helix	206-215	10
α-helix	224-238	15
α-helix	240-243	4
α-helix	252-259	8
β-strand	267-268	2	5
β-strand	269-273	5	8
α-helix	288-295	8
α-helix	298-300	3
β-strand	301	1	8
α-helix	307-309	3
β-strand	312-321	10	8
α-helix	325-337	13
α-helix	340-342	3
β-strand	343	1	8
β-strand	351-356	6	8
α-helix	359-360	2
β-strand	373-381	9	8
α-helix	382-384	3
α-helix	385-401	17
α-helix	405-410	6
α-helix	415-437	23

Chain C: 22 helices, 0 β-strands

Element	Residues	Length
α-helix	13-21	9
α-helix	22-24	3
α-helix	28-41	14
α-helix	44-46	3
α-helix	47-53	7
α-helix	59-72	14
α-helix	75-77	3
α-helix	78-84	7
α-helix	90-103	14
α-helix	106-108	3
α-helix	109-114	6
α-helix	115-117	3
α-helix	121-134	14
α-helix	137-139	3
α-helix	140-146	7
α-helix	152-165	14
α-helix	168-170	3
α-helix	171-176	6
α-helix	177-179	3
α-helix	183-196	14
α-helix	198-211	14
α-helix	214-224	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Alpha-tubulin	A	protein	451	Ovis aries
Tubulin beta chain	B	protein	445	Ovis aries	D0VWY9 (AlphaFold model)
iE5 alpharep	C	protein	232	synthetic construct

Sequence of entity 1 (A), FASTA

>6GWC_1 ALPHA-TUBULIN (chains A)
MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGK
HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLD
RIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTA
VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITA
SLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPAN
QMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPP
TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
AREDMAALEKDYEEVGVDSVEGEGEEEGEEY

Sequence of entity 2 (B), FASTA

>6GWC_2 Tubulin beta chain (chains B)
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYV
PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV
RKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVV
EPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCL
RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMM
AACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRG
LKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
EYQQYQDATADEQGEFEEEEGEDEA

Sequence of entity 3 (C), FASTA

>6GWC_3 iE5 ALPHAREP (chains C)
MRGSHHHHHHTDPEKVEMYIKNLQDDSTLVRSIAAAALGKIGDERAVEPLIKALKDEDSR
VRAQAAGALGQIGDERAVEPLIKALKDEDPSVRYRAAEALGKIGDERAVEPLIKALKDED
TTVRRIAATALGKIGDERAVEPLIKALKDEDAAVRLTAARALGEIGDERAVEPLIKALKD
EDAYVRRAAAQALGKIGGERVRAAMEKLAETGTGFARKVAVNYLETHKSLIS

Ligands and cofactors

ID	Name	Formula	Copies
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	2
MG	Magnesium ion	Mg	2

Water and common crystallization additives (PGE, MES) are not listed.

Primary citation

Selection and Characterization of Artificial Proteins Targeting the Tubulin alpha Subunit. Campanacci, V., Urvoas, A., Consolati, T. et al. Structure (2019) 27:497-506.e4. DOI 10.1016/j.str.2018.12.001 · PubMed

Other PDB entries of the same protein (UniProt D0VWY9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5EYP 1.9 Å, Tubulin-darpin complex
3RYC 2.1 Å, Tubulin: RB3 stathmin-like domain complex
6TH4 2.12 Å, Tubulin-inhibitor complex
4LNU 2.19 Å, Nucleotide-free kinesin motor domain in complex with tubulin and a DARPin
9F07 2.21 Å, Tubulin:stathmin:darpin:tau MTBR3 complex
4DRX 2.22 Å, GTP-Tubulin in complex with a DARPIN
6TDE 2.29 Å, Tubulin-inhibitor complex
7Q1F 2.35 Å, CPAP:TUBULIN:IE5 alpharep complex
7Z0F 2.4 Å, Cpap:s-TUBULIN:IIH5 alpharep complex
3RYI 2.4 Å, GDP-Tubulin: rb3 stathmin-like domain complex
5KX5 2.5 Å, Crystal structure of tubulin-stathmin-TTL-Compound 11 complex
3RYF 2.52 Å, GTP-Tubulin: RB3 Stathmin-like domain complex

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