6H8Q: Scc3-dependent cohesin recruitment to chromatin

Structural basis for Scc3-dependent cohesin recruitment to chromatin. Determined by X-ray diffraction at 3.63 Å resolution. Released 29 Aug 2018.

Method
X-ray diffraction
Resolution
3.63 Å
Organisms
Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Homo sapiens
Chains
8
Atoms
16,363
Mol. weight
312.81 kDa
Released
29 Aug 2018

Explore 6H8Q in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6H8Q contains 112 α-helices and 0 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 54 helices, 0 β-strands

ElementResiduesLengthSheet
α-helix150-15910
α-helix165-17915
α-helix187-1904
α-helix196-2027
α-helix205-2084
α-helix218-2225
α-helix232-2365
α-helix237-2437
α-helix245-2495
α-helix270-28213
α-helix288-31225
α-helix313-3175
α-helix318-3258
α-helix338-36124
α-helix362-3676
α-helix376-3849
α-helix386-3927
α-helix404-4096
α-helix416-42914
α-helix444-45916
α-helix466-48015
α-helix485-4928
α-helix493-4953
α-helix510-53627
α-helix538-5403
α-helix551-57020
α-helix574-5752
α-helix578-58912
α-helix600-6078
α-helix648-66417
α-helix674-6807
α-helix684-6907
α-helix696-70611
α-helix712-7154
α-helix721-73616
α-helix747-76115
α-helix764-78724
α-helix798-8058
α-helix806-8105
α-helix811-8166
α-helix826-83510
α-helix837-8393
α-helix841-8444
α-helix851-8544
α-helix858-86710
α-helix872-8754
α-helix889-91123
α-helix920-94627
α-helix953-96210
α-helix969-99022
α-helix1002-10054
α-helix1018-103215
α-helix1038-10447
α-helix1053-10608
Chain B: 54 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix150-16213
α-helix165-17915
α-helix187-1904
α-helix196-2027
α-helix205-2084
α-helix218-2225
α-helix232-2365
α-helix237-2437
α-helix271-28212
α-helix290-31223
α-helix313-3175
α-helix318-3258
α-helix338-36124
α-helix362-3676
α-helix376-3849
α-helix386-3927
α-helix404-4096
α-helix416-42813
α-helix444-45916
α-helix466-47914
α-helix485-4928
α-helix493-4953
α-helix510-53627
α-helix538-5403
α-helix551-57020
α-helix574-5752
α-helix578-58912
α-helix594-5963
α-helix600-6078
α-helix648-66417
α-helix674-6807
α-helix684-6907
α-helix696-70611
α-helix712-7154
α-helix721-73616
α-helix747-76014
α-helix764-78724
α-helix798-8058
α-helix806-8105
α-helix811-8166
α-helix826-8327
α-helix837-8393
α-helix841-8444
α-helix851-8544
α-helix859-86911
α-helix872-8754
α-helix894-91118
α-helix920-94627
α-helix953-96210
α-helix969-99022
α-helix1002-10054
α-helix1018-103215
α-helix1038-10447
α-helix1053-10597
Chain G: 2 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix363-37311
α-helix377-3804
Chain H: 2 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix364-3729
α-helix378-3836

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Cohesin subunit SCC3A, Bprotein1150Saccharomyces cerevisiae (strain ATCC 204508 / S288c)P40541 (AlphaFold model)
Sister chromatid cohesion protein 1G, Hprotein100Saccharomyces cerevisiae (strain ATCC 204508 / S288c)Q12158 (AlphaFold model)
DNA (5'-d(p*cp*tp*tp*tp*cp*gp*tp*tp*tp*cp*cp*tp*tp*gp*ap*ap*ap*ap*a)-3')EDNA19Homo sapiens
DNA (5'-d(p*cp*tp*tp*tp*cp*gp*tp*tp*tp*cp*cp*tp*tp*gp*ap*ap*ap*ap*a)-3')FDNA19Homo sapiens
DNA (5'-d(p*tp*tp*tp*tp*tp*cp*ap*ap*gp*gp*ap*ap*ap*cp*gp*ap*ap*ap*g)-3')CDNA19Homo sapiens
DNA (5'-d(p*cp*tp*tp*tp*cp*gp*tp*tp*tp*cp*cp*tp*tp*gp*ap*ap*ap*ap*a)-3')DDNA19Homo sapiens
Sequence of entity 1 (A, B), FASTA
>6H8Q_1 Cohesin subunit SCC3 (chains A, B)
MTAVRRSTRIRTKSQVIEEDYDDEQNTSAQHVESDKITAKTQHEEEEEQDTGESEESSSE
DDYEDQDDDDYVDTATAKRKSRKRKPKSASNTSSKRQKKKPTSAQKSAVSHAPAYHRSKK
DQDQYLEIAKDFQPTELFDILSTSEDVSIEELLREWLETYSENRDKFLQEFINLLLNCCG
SVARVEDHDVHSNESSNETIGEIQLLFQRQKLHEFYLLISKENKKRKNFKMGPLYQNFAE
FMTKLLEVANDLQLLYVESDEDDTQIVTGNLVLDLLTWLSSFSVCKIRCFRYISTLTLYL
FQDYLTQQAVNLEKNYLAKLSKQLSLEEKKKRPNNKTLEKLESTIAETQGSKVVIDSIID
NIVKLCFVHRYKDVSDLIRSESMLHLSIWIKNYPEYFLKVTFLKYFGWLLSDNSVSVRLQ
VTKILPHLIIQNHNSKSTDNSAIRQVFERFKTKILEVAIRDVNLDVRIHSIQVLTEASSL
GYLDDSEILIISSLMFDEEFDPFKTSSFNKRSKFLSTVAKFLARVIKEKFDEFIKTHEDL
PKEVDGLEVGPVVQVGIFIKILNDSLIYHLKDCAEVDSRTKIRMLTQAAEFLSPYISTHL
KTICNLLISDTESNELIQKLQNSANNNSDDEDVDDEELDITPLFPIDRNSTILYLNVFHG
LCAGANNPKIQTKDSVKEIVLPLFYDLLNAASIESADILCPLLESFITFSLDDWISIGYE
TELKKITDKTIKAFMDSTIGNSKVDMKYDIFAKFIHHIHHFEKKELQEKFLNQIATLKIH
LKKFLQEKMDPNNSRDDYKDLTCSLYELYINKLTILGRDYPIEVDEELLQLFLNNFVSRI
PIMFQDFDDSTAQEINFKMLVLLATWNLEKWREIIEKVRDYENSISKDLRSVWKPIAAII
GRLNTLVISLAATNETFENINSLFYLKWSACTSLMDIIVAIKIFELKLPADATTWRYSMS
EQFPFYLHDNASKVLLKIFLYLESLFAKQVDVQLERVADEDANLNDLPETGFFENIETEF
LLFTVKLKGLMKLNILDERFASRVALNKEKLGPLFKKIVDDTIMENPEPNKKNIQKAKSN
QTQREKAPLQPNSERETDHANTENNDPDIPMTIDLEPIEESSQNNSELAPIEEHPTVVDA
IDNSDEITQD
Sequence of entity 2 (G, H), FASTA
>6H8Q_2 Sister chromatid cohesion protein 1 (chains G, H)
TDAMTESQPKQTGTRRNSKLLNTKSIQIDEETENSESIASSNTYKEERSNNLLTPQPTNF
TTKRLWSEITESMSYLPDPILKNFLSYESLKKRKIHNGRE
Sequence of entity 3 (E), FASTA
>6H8Q_3 DNA (5'-D(P*CP*TP*TP*TP*CP*GP*TP*TP*TP*CP*CP*TP*TP*GP*AP*AP*AP*AP*A)-3') (chains E)
TTTTCAAGGAAACGAAACG
Sequence of entity 4 (F), FASTA
>6H8Q_4 DNA (5'-D(P*CP*TP*TP*TP*CP*GP*TP*TP*TP*CP*CP*TP*TP*GP*AP*AP*AP*AP*A)-3') (chains F)
CGTTTCGTTTCCTTGAAAA
Sequence of entity 5 (C), FASTA
>6H8Q_5 DNA (5'-D(P*TP*TP*TP*TP*TP*CP*AP*AP*GP*GP*AP*AP*AP*CP*GP*AP*AP*AP*G)-3') (chains C)
TTTTTCAAGGAAACGAAAG
Sequence of entity 6 (D), FASTA
>6H8Q_6 DNA (5'-D(P*CP*TP*TP*TP*CP*GP*TP*TP*TP*CP*CP*TP*TP*GP*AP*AP*AP*AP*A)-3') (chains D)
CTTTCGTTTCCTTGAAAAA

Primary citation

Structural basis for Scc3-dependent cohesin recruitment to chromatin. Li, Y., Muir, K., Bowler, M.W. et al. Elife (2018) 7. DOI 10.7554/eLife.38356 · PubMed

Other PDB entries of the same protein (UniProt P40541 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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