6I2M: Vaccinia virus protein A55 BTB-Back domain

Crystal structure of vaccinia virus protein A55 BTB-Back domain in complex with human Cullin-3 N-terminus. Determined by X-ray diffraction at 2.3 Å resolution. Released 23 Jan 2019.

Method
X-ray diffraction
Resolution
2.3 Å
Organisms
Vaccinia virus WR, Homo sapiens
Chains
2
Atoms
4,402
Mol. weight
75.68 kDa
Released
23 Jan 2019

Explore 6I2M in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6I2M contains 40 α-helices and 5 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 13 helices, 5 β-strands

ElementResiduesLengthSheet
α-helix4-1714
β-strand23-2751
β-strand30-3451
α-helix36-427
β-strand4312
α-helix44-496
β-strand61-6331
α-helix69-7911
α-helix82-843
α-helix91-10111
β-strand10312
α-helix104-11613
α-helix120-13213
α-helix136-14712
α-helix150-1534
α-helix157-1615
α-helix164-1718
α-helix184-19512
Chain B: 27 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix26-4520
α-helix49-513
α-helix54-6613
α-helix70-8415
α-helix85-895
α-helix90-945
α-helix101-12222
α-helix124-1252
α-helix126-1305
α-helix131-1333
α-helix139-1479
α-helix148-1525
α-helix155-17420
α-helix180-19213
α-helix199-2013
α-helix202-2065
α-helix207-22721
α-helix230-25122
α-helix254-2563
α-helix257-26812
α-helix270-2723
α-helix273-2775
α-helix284-2907
α-helix293-30311
α-helix309-32719
α-helix345-35814
α-helix364-38017

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Kelch repeat and BTB domain-containing protein A55Aprotein259Vaccinia virus WRP24768 (AlphaFold model)
Cullin-3Bprotein388Homo sapiensQ13618 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>6I2M_1 Kelch repeat and BTB domain-containing protein A55 (chains A)
MNNSSELIAVINGFRNSGRFCDISIVINDERINAHKLILSGASEYFSILFSNNFIDSNEY
EVNLSHLDYQSVNDLIDYIYGIPLSLTNDNVKYILSTADFLQIGSAITECENYILKNLCS
KNCIDFYIYADKYNNKKIESASFNTILQNILRLINDENFKYLTEESMIKILSDDMLNIKN
EDFAPLILIKWLESTQQSCTVELLRCLRISLLSPQVIKSLYSHQLVSSIYECITFLNNIA
FLDESFPRYHGSKHHHHHH
Sequence of entity 2 (B), FASTA
>6I2M_2 Cullin-3 (chains B)
MTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVT
EHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNL
GLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYE
EDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVK
VVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLR
EQGKALVSEEGEGKNPVDYRQGLDDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNS
RSPEYLAENLYFQSHHHHHHDYKDDDDK

Primary citation

Molecular basis of cullin-3 (Cul3) ubiquitin ligase subversion by vaccinia virus protein A55. Gao, C., Pallett, M.A., Croll, T.I. et al. J Biol Chem (2019) 294:6416-6429. DOI 10.1074/jbc.RA118.006561 · PubMed

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