Crystal structure of vaccinia virus protein A55 BTB-Back domain in complex with human Cullin-3 N-terminus. Determined by X-ray diffraction at 2.3 Å resolution. Released 23 Jan 2019.
Explore 6I2M in 3D Show helices and sheets RCSB PDB PDBe
6I2M contains 40 α-helices and 5 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 4-17 | 14 | |
| β-strand | 23-27 | 5 | 1 |
| β-strand | 30-34 | 5 | 1 |
| α-helix | 36-42 | 7 | |
| β-strand | 43 | 1 | 2 |
| α-helix | 44-49 | 6 | |
| β-strand | 61-63 | 3 | 1 |
| α-helix | 69-79 | 11 | |
| α-helix | 82-84 | 3 | |
| α-helix | 91-101 | 11 | |
| β-strand | 103 | 1 | 2 |
| α-helix | 104-116 | 13 | |
| α-helix | 120-132 | 13 | |
| α-helix | 136-147 | 12 | |
| α-helix | 150-153 | 4 | |
| α-helix | 157-161 | 5 | |
| α-helix | 164-171 | 8 | |
| α-helix | 184-195 | 12 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 26-45 | 20 | |
| α-helix | 49-51 | 3 | |
| α-helix | 54-66 | 13 | |
| α-helix | 70-84 | 15 | |
| α-helix | 85-89 | 5 | |
| α-helix | 90-94 | 5 | |
| α-helix | 101-122 | 22 | |
| α-helix | 124-125 | 2 | |
| α-helix | 126-130 | 5 | |
| α-helix | 131-133 | 3 | |
| α-helix | 139-147 | 9 | |
| α-helix | 148-152 | 5 | |
| α-helix | 155-174 | 20 | |
| α-helix | 180-192 | 13 | |
| α-helix | 199-201 | 3 | |
| α-helix | 202-206 | 5 | |
| α-helix | 207-227 | 21 | |
| α-helix | 230-251 | 22 | |
| α-helix | 254-256 | 3 | |
| α-helix | 257-268 | 12 | |
| α-helix | 270-272 | 3 | |
| α-helix | 273-277 | 5 | |
| α-helix | 284-290 | 7 | |
| α-helix | 293-303 | 11 | |
| α-helix | 309-327 | 19 | |
| α-helix | 345-358 | 14 | |
| α-helix | 364-380 | 17 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Kelch repeat and BTB domain-containing protein A55 | A | protein | 259 | Vaccinia virus WR | P24768 (AlphaFold model) |
| Cullin-3 | B | protein | 388 | Homo sapiens | Q13618 (AlphaFold model) |
>6I2M_1 Kelch repeat and BTB domain-containing protein A55 (chains A) MNNSSELIAVINGFRNSGRFCDISIVINDERINAHKLILSGASEYFSILFSNNFIDSNEY EVNLSHLDYQSVNDLIDYIYGIPLSLTNDNVKYILSTADFLQIGSAITECENYILKNLCS KNCIDFYIYADKYNNKKIESASFNTILQNILRLINDENFKYLTEESMIKILSDDMLNIKN EDFAPLILIKWLESTQQSCTVELLRCLRISLLSPQVIKSLYSHQLVSSIYECITFLNNIA FLDESFPRYHGSKHHHHHH
>6I2M_2 Cullin-3 (chains B) MTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVT EHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNL GLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYE EDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVK VVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLR EQGKALVSEEGEGKNPVDYRQGLDDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNS RSPEYLAENLYFQSHHHHHHDYKDDDDK
Molecular basis of cullin-3 (Cul3) ubiquitin ligase subversion by vaccinia virus protein A55. Gao, C., Pallett, M.A., Croll, T.I. et al. J Biol Chem (2019) 294:6416-6429. DOI 10.1074/jbc.RA118.006561 · PubMed
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