6ICZ: Human post-catalytic spliceosome

Cryo-EM structure of a human post-catalytic spliceosome (P complex) at 3.0 angstrom. Determined by electron microscopy at 3.0 Å resolution. Released 13 Mar 2019.

Method: Electron microscopy
Resolution: 3.0 Å
Organism: Homo sapiens
Chains: 51
Atoms: 97,900
Mol. weight: 2993.56 kDa
Ligands: IHP, GTP, MG, ZN
Released: 13 Mar 2019

Explore 6ICZ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6ICZ contains 653 α-helices and 520 β-strands across 47 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains a and h: 2 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	5-12	8
β-strand	17-22	6	75
β-strand	27-35	9	75
β-strand	41-49	9	75
β-strand	55-63	9	75
α-helix	65-67	3
β-strand	68-73	6	75

Chain A: 104 helices, 59 β-strands

Element	Residues	Length	Sheet
α-helix	27-44	18
α-helix	47-49	3
β-strand	60	1	27
α-helix	61-62	2
α-helix	63-73	11
α-helix	82-90	9
α-helix	91-94	4
α-helix	95-104	10
β-strand	115-121	7	27
β-strand	127-129	3	27
β-strand	134-135	2	28
α-helix	137-157	21
α-helix	174-177	4
α-helix	178-182	5
β-strand	228-229	2	29
α-helix	232-241	10
α-helix	260-269	10
α-helix	310-315	6
α-helix	329-331	3
α-helix	357-358	2
α-helix	371-373	3
β-strand	379	1	30
α-helix	380-381	2
α-helix	398-405	8
β-strand	415-416	2	29
β-strand	419-420	2	28
α-helix	428-432	5
α-helix	434-435	2
α-helix	441-459	19
α-helix	473-477	5
β-strand	482-488	7	27
α-helix	489-510	22
β-strand	516-518	3	31
β-strand	524-526	3	31
α-helix	529-531	3
α-helix	532-537	6
α-helix	542-565	24
α-helix	571-583	13
α-helix	585-588	4
α-helix	591-593	3
α-helix	596-598	3
α-helix	599-616	18
α-helix	631-661	31
α-helix	677-696	20
α-helix	706-721	16
α-helix	733-763	31
α-helix	769-797	29
α-helix	804-819	16
α-helix	822-824	3
β-strand	825	1	32
α-helix	827-829	3
α-helix	835-847	13
α-helix	858-872	15
α-helix	874-886	13
β-strand	894-900	7	33
β-strand	905-910	6	33
α-helix	913-932	20
β-strand	940	1	34
α-helix	948-960	13
β-strand	973-980	8	34
α-helix	985-987	3
α-helix	990-998	9
β-strand	1001	1	32
α-helix	1003-1011	9
β-strand	1016-1018	3	33
β-strand	1023-1025	3	33
α-helix	1037-1053	17
α-helix	1055-1062	8
α-helix	1077-1080	4
β-strand	1085-1091	7	34
β-strand	1094-1101	8	34
α-helix	1103-1116	14
α-helix	1140-1141	2
α-helix	1144-1158	15
α-helix	1170-1172	3
β-strand	1174-1178	5	34
β-strand	1185-1187	3	35
β-strand	1192-1198	7	35
β-strand	1213-1217	5	35
β-strand	1224-1232	9	35
α-helix	1234-1249	16
α-helix	1256-1274	19
α-helix	1277-1280	4
α-helix	1282-1302	21
α-helix	1313-1317	5
α-helix	1320-1322	3
α-helix	1327-1329	3
β-strand	1335-1337	3	36
α-helix	1340-1343	4
β-strand	1352-1354	3	36
α-helix	1369-1371	3
α-helix	1375-1398	24
α-helix	1419-1422	4
α-helix	1436-1442	7
α-helix	1443-1445	3
α-helix	1468-1477	10
α-helix	1480-1485	6
α-helix	1490-1493	4
α-helix	1510-1515	6
α-helix	1522-1537	16
α-helix	1539-1542	4
β-strand	1552-1554	3	37
β-strand	1561-1563	3	37
α-helix	1567-1576	10
α-helix	1581-1597	17
β-strand	1606-1611	6	38
β-strand	1629-1639	11	38
β-strand	1643-1644	2	38
β-strand	1655-1667	13	38
α-helix	1676-1688	13
β-strand	1700-1706	7	38
β-strand	1711-1716	6	38
α-helix	1722-1736	15
α-helix	1738-1751	14
α-helix	1768-1771	4
β-strand	1777-1781	5	39
β-strand	1788-1792	5	40
β-strand	1798-1802	5	40
β-strand	1805-1810	6	39
β-strand	1816-1822	7	39
α-helix	1824-1827	4
α-helix	1833-1851	19
β-strand	1860-1863	4	39
α-helix	1866-1868	3
α-helix	1869-1875	7
β-strand	1883-1886	4	39
α-helix	1893-1898	6
α-helix	1900-1907	8
β-strand	1913-1918	6	39
α-helix	1923-1925	3
α-helix	1929-1945	17
α-helix	1949-1954	6
α-helix	1973-1995	23
α-helix	1999-2001	3
α-helix	2004-2012	9
α-helix	2071-2080	10
α-helix	2083-2086	4
β-strand	2090-2092	3	41
β-strand	2103-2107	5	41
α-helix	2108-2117	10
β-strand	2125-2131	7	41
α-helix	2132-2133	2
β-strand	2139-2146	8	41
β-strand	2150	1	42
β-strand	2151-2152	2	43
β-strand	2157-2158	2	43
α-helix	2163-2164	2
α-helix	2167-2169	3
β-strand	2173-2181	9	41
α-helix	2190-2202	13
β-strand	2212-2219	8	41
β-strand	2222-2230	9	41
α-helix	2232-2240	9
α-helix	2253-2255	3
β-strand	2256-2258	3	41
β-strand	2260-2264	5	41
β-strand	2270-2274	5	41
β-strand	2280	1	42
β-strand	2296-2298	3	41
α-helix	2300-2303	4
α-helix	2307-2309	3
α-helix	2311-2314	4
α-helix	2321-2325	5

Chains b and i: 3 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	7-10	4
β-strand	16-21	6	75
β-strand	26-33	8	75
β-strand	40-48	9	75
β-strand	64-72	9	75
α-helix	74-76	3
β-strand	77-84	8	75
α-helix	85-87	3

Chains c and j: 4 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	3-8	6
β-strand	14-19	6	75
β-strand	24-32	9	75
β-strand	38-46	9	75
β-strand	53-60	8	75
α-helix	62-64	3
β-strand	65-69	5	75
α-helix	70-71	2
α-helix	76-79	4

Chain C: 38 helices, 43 β-strands

Element	Residues	Length	Sheet
α-helix	69-72	4
β-strand	78-81	4	44
α-helix	118-123	6
β-strand	129-136	8	45
α-helix	142-153	12
α-helix	174-177	4
β-strand	184-191	8	45
β-strand	197-204	8	45
α-helix	209-211	3
α-helix	212-221	10
β-strand	224-230	7	45
α-helix	237-247	11
β-strand	252-258	7	45
α-helix	260-263	4
α-helix	271-292	22
β-strand	307-311	5	45
β-strand	316-318	3	45
α-helix	320-330	11
α-helix	336-340	5
β-strand	347-350	4	30
β-strand	355-358	4	30
α-helix	368-369	2
α-helix	370-374	5
α-helix	375-385	11
α-helix	392-398	7
α-helix	407-409	3
α-helix	412-424	13
α-helix	428-437	10
α-helix	441-452	12
β-strand	453	1	46
α-helix	462-465	4
β-strand	475-483	9	47
β-strand	490-498	9	47
β-strand	501-502	2	48
β-strand	506	1	49
β-strand	509	1	50
β-strand	510	1	47
β-strand	523	1	50
β-strand	526	1	49
β-strand	530-531	2	47
β-strand	533-534	2	45
β-strand	537-538	2	45
β-strand	541	1	47
β-strand	543-544	2	48
β-strand	549-553	5	47
β-strand	563-565	3	47
β-strand	577	1	46
α-helix	578-579	2
β-strand	588-594	7	51
α-helix	597-599	3
α-helix	600-613	14
β-strand	618-621	4	51
β-strand	627-631	5	51
α-helix	634-642	9
α-helix	643-647	5
β-strand	653-655	3	51
α-helix	656-658	3
β-strand	659	1	51
β-strand	663-666	4	52
β-strand	675-676	2	53
β-strand	683-691	9	53
α-helix	692-693	2
α-helix	694-701	8
α-helix	711-721	11
α-helix	726-730	5
β-strand	734	1	53
β-strand	743-747	5	53
α-helix	756-774	19
β-strand	783-784	2	52
β-strand	787-796	10	53
α-helix	812-821	10
β-strand	825-838	14	52
α-helix	844-852	9
β-strand	859-862	4	52
α-helix	863	1
β-strand	869-876	8	52
α-helix	878-880	3
α-helix	883-890	8
β-strand	896-906	11	52
α-helix	926-928	3
α-helix	929-941	13

Chain d: 3 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	21-26	6
α-helix	30-38	9
β-strand	41-46	6	75
β-strand	51-59	9	75
β-strand	65-76	12	75
β-strand	90-101	12	75
α-helix	103-105	3
β-strand	106-111	6	75

Chain D: 89 helices, 70 β-strands

Element	Residues	Length	Sheet
β-strand	435-438	4	109
β-strand	442-447	6	109
α-helix	452-456	5
β-strand	463	1	110
β-strand	480	1	110
α-helix	483-494	12
β-strand	499-502	4	111
α-helix	510-523	14
β-strand	537-541	5	111
α-helix	545-559	15
β-strand	568	1	111
α-helix	580-582	3
β-strand	586-588	3	111
α-helix	590-599	10
β-strand	609-614	6	111
α-helix	617-621	5
α-helix	625-642	18
β-strand	647-652	6	111
α-helix	658-664	7
α-helix	669-672	4
β-strand	673-675	3	111
α-helix	678-680	3
β-strand	685-692	8	109
α-helix	697-714	18
β-strand	721-724	4	109
α-helix	730-734	5
α-helix	737-744	8
α-helix	761-766	6
α-helix	774-777	4
β-strand	782-784	3	109
α-helix	792-802	11
β-strand	808-811	4	109
α-helix	813-817	5
β-strand	823	1	112
β-strand	825-829	5	109
β-strand	832-835	4	113
β-strand	840-843	4	113
α-helix	844-845	2
α-helix	846-853	8
β-strand	856	1	112
β-strand	865-872	8	109
α-helix	873-875	3
α-helix	876-883	8
α-helix	893-906	14
β-strand	912	1	114
α-helix	913-920	8
α-helix	924-931	8
α-helix	933-936	4
α-helix	940-945	6
α-helix	950-955	6
α-helix	958-966	9
β-strand	970-972	3	115
β-strand	978	1	114
β-strand	979-981	3	115
α-helix	983-991	9
α-helix	995-1004	10
α-helix	1011-1019	9
α-helix	1022-1024	3
α-helix	1031-1033	3
α-helix	1034-1042	9
α-helix	1055-1067	13
α-helix	1075-1102	28
β-strand	1104	1	116
α-helix	1105-1120	16
α-helix	1128-1131	4
α-helix	1137-1145	9
α-helix	1150-1152	3
α-helix	1158-1162	5
α-helix	1168-1170	3
α-helix	1171-1180	10
β-strand	1184-1194	11	117
β-strand	1197-1206	10	117
β-strand	1218-1226	9	118
β-strand	1232	1	116
β-strand	1233-1242	10	118
α-helix	1249	1
β-strand	1250-1257	8	117
β-strand	1265-1272	8	118
β-strand	1279-1285	7	118
α-helix	1291-1302	12
α-helix	1305-1307	3
β-strand	1308	1	119
α-helix	1315-1318	4
β-strand	1327	1	119
α-helix	1330-1339	10
β-strand	1346-1349	4	120
α-helix	1356-1370	15
β-strand	1376-1379	4	120
α-helix	1383-1393	11
α-helix	1394-1399	6
α-helix	1400-1401	2
β-strand	1405-1407	3	120
α-helix	1412-1418	7
β-strand	1419	1	121
β-strand	1422	1	121
β-strand	1424-1427	4	120
α-helix	1431-1437	7
α-helix	1443-1446	4
β-strand	1450-1454	5	120
α-helix	1456-1460	5
α-helix	1465-1480	16
β-strand	1486-1491	6	120
β-strand	1494	1	122
α-helix	1497-1504	8
α-helix	1508-1510	3
β-strand	1511-1513	3	120
β-strand	1523-1530	8	123
α-helix	1535-1541	7
α-helix	1543-1553	11
β-strand	1559-1563	5	123
α-helix	1568-1582	15
β-strand	1590	1	124
α-helix	1594-1601	8
α-helix	1607-1614	8
β-strand	1619-1620	2	123
α-helix	1626-1638	13
β-strand	1641	1	124
β-strand	1643-1647	5	123
α-helix	1648-1651	4
β-strand	1658	1	125
β-strand	1660-1664	5	123
β-strand	1667-1670	4	126
β-strand	1675-1678	4	126
α-helix	1679-1680	2
α-helix	1681-1689	9
β-strand	1691	1	125
β-strand	1700-1707	8	123
α-helix	1711-1717	7
α-helix	1721	1
β-strand	1722	1	122
α-helix	1723	1
α-helix	1733-1741	9
β-strand	1747	1	127
α-helix	1748-1755	8
α-helix	1759-1764	6
α-helix	1768-1770	3
α-helix	1778-1798	21
β-strand	1802-1805	4	127
β-strand	1809-1812	4	127
α-helix	1814-1822	9
α-helix	1826-1835	10
α-helix	1842-1850	9
α-helix	1853-1855	3
α-helix	1867-1873	7
α-helix	1887-1899	13
α-helix	1906-1933	28
β-strand	1935	1	128
α-helix	1936-1951	16
α-helix	1960-1962	3
α-helix	1968-1977	10
α-helix	2001-2013	13
β-strand	2017-2023	7	129
β-strand	2034-2043	10	129
β-strand	2064-2070	7	128
α-helix	2071-2073	3
β-strand	2075-2082	8	128
β-strand	2087-2095	9	129
β-strand	2102-2110	9	128
β-strand	2118-2124	7	128

Chain e: 2 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	17-27	11
β-strand	30-35	6	75
β-strand	38-50	13	75
β-strand	56-65	10	75
β-strand	70-79	10	75
α-helix	81-83	3
β-strand	84-90	7	75

35 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Protein mago nashi homolog 2	v	protein	148	Homo sapiens	Q96A72 (AlphaFold model)
RNA-binding protein 8A	w	protein	174	Homo sapiens	Q9Y5S9 (AlphaFold model)
Eukaryotic initiation factor 4A-III	u	protein	411	Homo sapiens	P38919 (AlphaFold model)
Protein CASC3	x	protein	703	Homo sapiens	O15234 (AlphaFold model)
Pre-mRNA-processing-splicing factor 8	A	protein	2335	Homo sapiens	Q6P2Q9
U5snRNA	B	RNA	117	Homo sapiens
116 kDa U5 small nuclear ribonucleoprotein component	C	protein	972	Homo sapiens	Q15029
U5 small nuclear ribonucleoprotein 40 kDa protein	E	protein	357	Homo sapiens	Q96DI7
U6snRNA	F	RNA	107	Homo sapiens
pre-mRNA	G	RNA	273	Homo sapiens
U2snRNA	H	RNA	188	Homo sapiens
Crooked neck-like protein 1	J	protein	848	Homo sapiens	Q9BZJ0

29 more molecules are not listed.

Sequence of entity 1 (v), FASTA

>6ICZ_1 Protein mago nashi homolog 2 (chains v)
MAVASDFYLRYYVGHKGKFGHEFLEFEFRPDGKLRYANNSNYKNDVMIRKEAYVHKSVME
ELKRIIDDSEITKEDDALWPPPDRVGRQELEIVIGDEHISFTTSKIGSLIDVNQSKDPEG
LRVFYYLVQDLKCLVFSLIGLHFKIKPI

Sequence of entity 2 (w), FASTA

>6ICZ_2 RNA-binding protein 8A (chains w)
MADVLDLHEAGGEDFAMDEDGDESIHKLKEKAKKRKGRGFGSEEGSRARMREDYDSVEQD
GDEPGPQRSVEGWILFVTGVHEEATEEDIHDKFAEYGEIKNIHLNLDRRTGYLKGYTLVE
YETYKEAQAAMEGLNGQDLMGQPISVDWCFVRGPPKGKRRGGRRRSRSPDRRRR

Sequence of entity 3 (u), FASTA

>6ICZ_3 Eukaryotic initiation factor 4A-III (chains u)
MATTATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEK
PSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAV
QIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRA
IKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRIL
VKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREA
NFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNREL
YIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI

Sequence of entity 4 (x), FASTA

>6ICZ_4 Protein CASC3 (chains x)
MADRRRQRASQDTEDEESGASGSDSGGSPLRGGGSCSGSAGGGGSGSLPSQRGGRTGALH
LRRVESGGAKSAEESECESEDGIEGDAVLSDYESAEDSEGEEGEYSEEENSKVELKSEAN
DAVNSSTKEEKGEEKPDTKSTVTGERQSGDGQESTEPVENKVGKKGPKHLDDDEDRKNPA
YIPRKGLFFEHDLRGQTQEEEVRPKGRQRKLWKDEGRWEHDKFREDEQAPKSRQELIALY
GYDIRSAHNPDDIKPRRIRKPRYGSPPQRDPNWNGERLNKSHRHQGLGGTLPPRTFINRN
AAGTGRMSAPRNYSRSGGFKEGRAGFRPVEAGGQHGGRSGETVKHEISYRSRRLEQTSVR
DPSPEADAPVLGSPEKEEAASEPPAAAPDAAPPPPDRPIEKKSYSRARRTRTKVGDAVKL
AEEVPPPPEGLIPAPPVPETTPTPPTKTGTWEAPVDSSTSGLEQDVAQLNIAEQNWSPGQ
PSFLQPRELRGMPNHIHMGAGPPPQFNRMEEMGVQGGRAKRYSSQRQRPVPEPPAPPVHI
SIMEGHYYDPLQFQGPIYTHGDSPAPLPPQGMLVQPGMNLPHPGLHPHQTPAPLPNPGLY
PPPVSMSPGQPPPQQLLAPTYFSAPGVMNFGNPSYPYAPGALPPPPPPHLYPNTQAPSQV
YGGVTYYNPAQQQVQPKPSPPRRTPQPVTIKPPPPEVVSRGSS

Sequence of entity 5 (A), FASTA

>6ICZ_5 Pre-mRNA-processing-splicing factor 8 (chains A)
MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKED
MPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLY
HITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYAD
NILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYR
LANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDIN
KIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPIS
HRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR
ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATK
FFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRF
GNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMR
QIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQF
EGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCW
KANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLT
RLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI
LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMD
LYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGIN
NLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYK
DMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATE
AAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARM
RLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKC
RTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV
NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPK
ELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFI
DSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRT
DFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEG
LFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGI
FMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSY
KMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA
RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPAL
YVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTT
KPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQ
IIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLY
DDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVE
VQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLT
ATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET
GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLP
GQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTP
GSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWN
YNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Sequence of entity 6 (B), FASTA

>6ICZ_6 U5snRNA (chains B)
AUACUCUGGUUUCUCUUCAGAUCGCAUAAAUCUUUCGCCUUUUACUAAAGAUUUCCGUGG
AGAGGAACAACUCUGAGUCUUAACCCAAUUUUUUGAGGCCUUGCUUUGGCAAGGCUA

Sequence of entity 7 (C), FASTA

>6ICZ_7 116 kDa U5 small nuclear ribonucleoprotein component (chains C)
MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLH
EDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLA
DLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVG
IKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTE
RLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLIL
SPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKA
PTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVC
KKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTK
MYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIE
VNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSEL
PKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVV
TFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQT
KYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLC
DELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPA
DCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSV
FHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLE
LAKQDVVLNYPM

Sequence of entity 8 (E), FASTA

>6ICZ_8 U5 small nuclear ribonucleoprotein 40 kDa protein (chains E)
MIEQQKRKGPELPLVPVKRQRHELLLGAGSGPGAGQQQATPGALLQAGPPRCSSLQAPIM
LLSGHEGEVYCCKFHPNGSTLASAGFDRLILLWNVYGDCDNYATLKGHSGAVMELHYNTD
GSMLFSASTDKTVAVWDSETGERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWD
IRKKAAIQTFQNTYQVLAVTFNDTSDQIISGGIDNDIKVWDLRQNKLTYTMRGHADSVTG
LSLSSEGSYLLSNAMDNTVRVWDVRPFAPKERCVKIFQGNVHNFEKNLLRCSWSPDGSKI
AAGSADRFVYVWDTTSRRILYKLPGHAGSINEVAFHPDEPIIISASSDKRLYMGEIQ

Sequence of entity 9 (F), FASTA

>6ICZ_9 U6snRNA (chains F)
GUGCUCGCUUCGGCAGCACAUAUACUAAAAUUGGAACGAUACAGAGAAGAUUAGCAUGGC
CCCUGCGCAAGGAUGACACGCAAAUUCGUGAAGCGUUCCAUAUUUUU

Sequence of entity 10 (G), FASTA

>6ICZ_10 pre-mRNA (chains G)
GAAUACACGGAAUUCGAGCUCGCCCACUCUUGGAUCGGAAACCCGUCGGCCUCCGAACGN
GUAAGAGCCUAGCAUGUAGAACUGGUUACCUGCAGCCCAAGCUUGCGUACACCAUCAGGG
UACGUACUAGUACGUACACCAUCAGGGUACGGCUGCACGUCUAGGGCGCAGUAGUCCAGG
GUUUCCUUGAUGAUGUCAUACUUAUCCUGUCCCUUUUUUUUCCACAGCUCGCGGUUGAGG
ACAAACUCUUCGCGGUCUUUCCAGUGGGGAUCC

Sequence of entity 11 (H), FASTA

>6ICZ_11 U2snRNA (chains H)
AUCGCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGUUUAAUAU
CUGAUACGUCCUCUAUCCGAGGACAAUAUAUUAAAUGGAUUUUUGGAGCAGGGAGAUGGA
AUAGGAGCUUGCUCCGUCCACUCCACGCAUCGACCUGGUAUUGCAGUACCUCCAGGAACG
GUGCACCC

Sequence of entity 12 (J), FASTA

>6ICZ_12 Crooked neck-like protein 1 (chains J)
MTATVENLTFQKDTLGNAVDKNTSRLELRSYSLAGRHGSTEPLVLAWSSQFRRLTWGCAL
DALHRSPCVAASQHGVTHLIRSSRTPHSTRCRKEDAQPGHHGNGAASVTAQARGQRSVLQ
VPLPVPRSCLFSESFVVSVSSQSRFLASVPGTGVQRSTAADMAASTAAGKQRIPKVAKVK
NKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKTFEDNIRKNRT
VISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQVNHARNIWD
RAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVD
RARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFA
KFEENQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQY
EEEVKANPHNYDAWFDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINY
ALYEELEAKDPERTRQVYQASLELIPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSI
GKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTSWIKFAELETILGDIDRAR
AIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQRTQHVKVWISFAQFEL
SSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPE
KVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPD
EDVDESES

Ligands and cofactors

ID	Name	Formula	Copies
IHP	Inositol hexakisphosphate	C6 H18 O24 P6	1
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	1
MG	Magnesium ion	Mg	9
ZN	Zinc ion	Zn	7
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	1

Primary citation

Structures of the human spliceosomes before and after release of the ligated exon. Zhang, X., Zhan, X., Yan, C. et al. Cell Res (2019) 29:274-285. DOI 10.1038/s41422-019-0143-x · PubMed

Other PDB entries of the same protein (UniProt Q96A72 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6QDV 3.3 Å, Human post-catalytic P complex spliceosome
5XJC 3.6 Å, Cryo-EM structure of the human spliceosome just prior to exon ligation at 3.6 angstrom
5YZG 4.1 Å, The Cryo-EM Structure of Human Catalytic Step I Spliceosome (C complex) at 4.1 angstrom…

Browse structure collections

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