6PWN: MscS Nanodisc with N-terminal His-Tag

MscS Nanodisc with N-terminal His-Tag. Determined by electron microscopy at 3.1 Å resolution. Released 8 Jan 2020.

Method: Electron microscopy
Resolution: 3.1 Å
Organism: Escherichia coli (strain K12)
Chains: 7
Atoms: 15,379
Mol. weight: 238.57 kDa
Ligands: R16, POV
Released: 8 Jan 2020

Explore 6PWN in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6PWN contains 56 α-helices and 63 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A, B, C, D, E, F and G: 8 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	2-6	5
α-helix	21-57	37
α-helix	63-89	27
α-helix	94-127	34
β-strand	135-138	4	1
β-strand	142-148	7	1
β-strand	152-157	6	1
β-strand	162-166	5	1
α-helix	167-170	4
β-strand	175-177	3	1
β-strand	183-192	10	2
α-helix	198-211	14
β-strand	222-228	7	2
β-strand	233-242	10	2
α-helix	246-264	19
α-helix	268-271	4
β-strand	272-279	8	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Small-conductance mechanosensitive channel	A, B, C, D, E, F, G	protein	306	Escherichia coli (strain K12)	P0C0S1 (AlphaFold model)

Sequence of entity 1 (A, B, C, D, E, F, G), FASTA

>6PWN_1 Small-conductance mechanosensitive channel (chains A, B, C, D, E, F, G)
MGSSHHHHHHSSGLVPRGSHMEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIV
GLIIARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVL
GAAGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKDRE
MTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVNFKRV
KEDKAA

Ligands and cofactors

ID	Name	Formula	Copies
R16	Hexadecane	C16 H34	7
POV	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl…	C42 H82 N O8 P	7

Primary citation

Molecular basis of force-from-lipids gating in the mechanosensitive channel MscS. Reddy, B., Bavi, N., Lu, A. et al. Elife (2019) 8. DOI 10.7554/eLife.50486 · PubMed

Other PDB entries of the same protein (UniProt P0C0S1 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7ONJ 2.3 Å, Mechanosensitive channel MscS solubilized with LMNG in open conformation
9E68 2.5 Å, Cryo-EM structure of MscS/YnaI chimera in DOPC nanodiscs
7OOA 2.7 Å, Mechanosensitive channel MscS solubilized with LMNG in open conformation with added lipid
9H2S 2.7 Å, a YnaI-MscS chimera in a closed conformation purified in DDM with additional lipids…
9H2V 2.8 Å, a YnaI-MscS chimera in an open conformation purified in DDM showing ligand-filled pockets
6RLD 2.9 Å, Structure of the mechanosensitive channel mscs embedded in the membrane bilayer
5AJI 2.99 Å, MscS D67R1 high resolution
7OO0 3.1 Å, Mechanosensitive channel MscS solubilized with DDM in open conformation
7OO6 3.1 Å, Mechanosensitive channel MscS solubilized with DDM in closed conformation with added lipid
8DDJ 3.1 Å, Open MscS in PC14.1 Nanodiscs
6VYK 3.2 Å, Cryo-EM structure of mechanosensitive channel MscS in PC-18:1 nanodiscs
6UZH 3.3 Å, Cryo-EM structure of mechanosensitive channel MscS reconstituted into peptidiscs

Browse structure collections

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