6RFO: PDB entry 6RFO

ERK2 MAP kinase with the activation loop of p38alpha. Determined by X-ray diffraction at 1.7 Å resolution. Released 27 May 2020.

Method: X-ray diffraction
Resolution: 1.7 Å
Organisms: Rattus norvegicus, Homo sapiens
Chains: 1
Atoms: 2,762
Mol. weight: 42.86 kDa
Released: 27 May 2020

Explore 6RFO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6RFO contains 22 α-helices and 11 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 22 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	23-27	5	1
β-strand	37-42	6	1
β-strand	48-54	7	1
α-helix	60-75	16
β-strand	81	1	2
β-strand	86-88	3	1
β-strand	99-104	6	1
β-strand	108-109	2	2
α-helix	110-116	7
α-helix	118-120	3
α-helix	121-140	20
β-strand	143-144	2	3
α-helix	150-152	3
β-strand	153-155	3	2
β-strand	161-163	3	2
β-strand	170-171	2	3
α-helix	189-191	3
α-helix	194-198	5
α-helix	206-221	16
α-helix	231-242	12
α-helix	245-246	2
α-helix	247-251	5
α-helix	256-264	9
α-helix	266-267	2
α-helix	269-272	4
α-helix	273-276	4
α-helix	282-291	10
α-helix	300-301	2
α-helix	302-306	5
α-helix	309-311	3
α-helix	317-319	3
α-helix	338-348	11
α-helix	350-352	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Mitogen-activated protein kinase 1,Mitogen-activated protein kinase 14,Mitogen-activated protein…	A	protein	372	Rattus norvegicus, Homo sapiens	P63086 (AlphaFold model), Q16539 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>6RFO_1 Mitogen-activated protein kinase 1,Mitogen-activated protein kinase 14,Mitogen-activated protein kinase 1 (chains A)
MGSSHHHHHHSSGLVPRGSHMAAAAAAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSA
YDNLNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVY
IVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCD
LKILDFGLARHTDDEMTGYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIF
PGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALD
LLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIF
EETARFQPGYRS

Primary citation

The bacterial metalloprotease NleD selectively cleaves mitogen-activated protein kinases that have high flexibility in their activation loop. Gur-Arie, L., Eitan-Wexler, M., Weinberger, N. et al. J Biol Chem (2020) 295:9409-9420. DOI 10.1074/jbc.RA120.013590 · PubMed

Other PDB entries of the same protein (UniProt P63086 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4S32 1.34 Å, Crystal structure of ERK2 AMP-PNP complex
4S31 1.45 Å, Crystal structure of mitogen-activated protein kinase 1 wtERK2 at 1.45A
5HD4 1.45 Å, Dissecting Therapeutic Resistance to ERK Inhibition Rat Wild Type SCH772984 in complex…
6DCG 1.45 Å, Discovery of MK-8353: An Orally Bioavailable Dual Mechanism ERK Inhibitor for Oncology
3QYZ 1.46 Å, Crystal structure of ERK2 in complex with an inhibitor
4XP0 1.46 Å, Crystal structure of ERK2 in complex with an inhibitor
4S2Z 1.48 Å, ERK2 Intrinsically active mutant R65S
4S33 1.48 Å, ERK2 R65S mutant complexed with AMP-PNP
6FLE 1.48 Å, Crystal structure of ERK2 in complex with an adenosine derivative
3QYW 1.5 Å, Crystal structure of ERK2 in complex with an inhibitor
8QR7 1.51 Å, Crystal structure of ERK2 in complex with a covalently bound macrocyclic ligand
8QRB 1.51 Å, Crystal structure of ERK2 in complex with a covalently bound macrocyclic ligand

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