6TJK: Recombinant GBA

Crystal Structure of Recombinant GBA in Complex with Bis-Tris Propane. Determined by X-ray diffraction at 1.56 Å resolution. Released 10 Jun 2020.

Method: X-ray diffraction
Resolution: 1.56 Å
Organism: Homo sapiens
Chains: 2
Atoms: 9,312
Mol. weight: 117.67 kDa
Ligands: NAG, B3P
Released: 10 Jun 2020

Explore 6TJK in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6TJK contains 53 α-helices and 52 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain AAA: 28 helices, 25 β-strands

Element	Residues	Length	Sheet
α-helix	2-4	3
β-strand	6-7	2	1
α-helix	14	1
β-strand	15-18	4	1
α-helix	26-29	4
α-helix	32-33	2
β-strand	36-43	8	2
β-strand	50-55	6	2
α-helix	56	1
β-strand	57	1	2
β-strand	65-77	13	2
α-helix	78	1
β-strand	80-84	5	3
α-helix	87-95	9
α-helix	98-109	12
β-strand	118-123	6	3
α-helix	151	1
α-helix	152-157	6
α-helix	158-167	10
α-helix	171-172	2
β-strand	173-178	6	3
α-helix	183-185	3
β-strand	186	1	4
β-strand	197	1	4
α-helix	204-222	19
β-strand	229-231	3	3
α-helix	236-240	5
α-helix	253-259	7
α-helix	260-264	5
α-helix	265-269	5
β-strand	277-284	8	3
α-helix	285-287	3
α-helix	290-296	7
α-helix	299-302	4
β-strand	307-311	5	3
α-helix	315-317	3
α-helix	320	1
α-helix	321-325	5
α-helix	326-330	5
β-strand	335-340	6	3
α-helix	357-372	16
β-strand	375-382	8	3
β-strand	385	1	1
β-strand	402-405	4	1
α-helix	406-408	3
β-strand	410-413	4	1
α-helix	415-424	10
β-strand	432-438	7	2
β-strand	444-450	7	2
β-strand	456-462	7	2
β-strand	468-474	7	2
β-strand	478-484	7	2
β-strand	488-494	7	2

Chain BBB: 25 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	2	1	5
β-strand	6-8	3	6
β-strand	14-18	5	6
β-strand	25	1	5
α-helix	26-29	4
α-helix	31-33	3
β-strand	36-43	8	7
β-strand	50-55	6	7
β-strand	57	1	7
β-strand	66-77	12	7
α-helix	78	1
β-strand	80-84	5	8
α-helix	87-94	8
α-helix	98-109	12
β-strand	118-123	6	8
α-helix	151	1
α-helix	152-157	6
α-helix	158-167	10
α-helix	171-172	2
β-strand	173-178	6	8
α-helix	183-185	3
β-strand	186	1	9
β-strand	197	1	9
α-helix	204-222	19
β-strand	229-231	3	8
α-helix	236-240	5
α-helix	253-259	7
α-helix	260-264	5
α-helix	265-269	5
β-strand	277-284	8	8
α-helix	285-287	3
α-helix	290-296	7
α-helix	299-302	4
β-strand	307-311	5	8
α-helix	315-317	3
α-helix	320	1
α-helix	321-325	5
α-helix	326-330	5
β-strand	335-340	6	8
α-helix	357-372	16
β-strand	375-382	8	8
β-strand	385	1	6
β-strand	402-405	4	6
α-helix	406-408	3
β-strand	410-413	4	6
α-helix	415-424	10
β-strand	432-438	7	7
β-strand	445-450	6	7
β-strand	456-462	7	7
β-strand	468-474	7	7
β-strand	478-484	7	7
β-strand	488-494	7	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Lysosomal acid glucosylceramidase	AAA, BBB	protein	497	Homo sapiens	P04062 (AlphaFold model)

Sequence of entity 1 (AAA, BBB), FASTA

>6TJK_1 Lysosomal acid glucosylceramidase (chains AAA, BBB)
ARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANH
TGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIR
VPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWT
SPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGL
LSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPE
AAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRG
MQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHL
GHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFL
ETISPGYSIHTYLWRRQ

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	3
B3P	2-[3-(2-hydroxy-1,1-dihydroxymethyl-ethylamino)-propylamino]-2-hydroxymethyl-pr…	C11 H26 N2 O6	2

Water and common crystallization additives (SO4, EDO) are not listed.

Primary citation

A baculoviral system for the production of human beta-glucocerebrosidase enables atomic resolution analysis. Rowland, R.J., Wu, L., Liu, F. et al. Acta Crystallogr D Struct Biol (2020) 76:565-580. DOI 10.1107/S205979832000501X · PubMed

Other PDB entries of the same protein (UniProt P04062 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6TN1 0.98 Å, Unliganded Crystal Structure of Recombinant GBA
9FB2 1.14 Å, Gcase in complex with small molecule inhibitor 1
9FA3 1.36 Å, Gcase in complex with small molecule inhibitor 1
9FAL 1.39 Å, Gcase in complex with small molecule inhibitor 1
9FAY 1.4 Å, Gcase in complex with small molecule inhibitor 1
6TJQ 1.41 Å, Crystal Structure of Recombinant GBA in Complex with 2-Deoxy-2-fluoro-beta-D-glucopyranos…
9FDI 1.41 Å, Gcase in complex with small molecule inhibitor 1
8AWR 1.49 Å, Structure of recombinant human beta-glucocerebrosidase in complex with L-carbaxylosyl…
9FA6 1.49 Å, Gcase in complex with small molecule inhibitor 1
8AWK 1.58 Å, Structure of recombinant human beta-glucocerebrosidase in complex with D-carbaxylosyl…
6TJJ 1.59 Å, Structure of Cerezyme at pH 4.6
8AX3 1.59 Å, Structure of recombinant human beta-glucocerebrosidase in complex with L-carbaxylosyl…

Browse structure collections

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