6UE8: Tetrameric sIgA complex

Structure of tetrameric sIgA complex (Class 1). Determined by electron microscopy at 3.0 Å resolution. Released 19 Feb 2020.

Method: Electron microscopy
Resolution: 3.0 Å
Organism: Homo sapiens
Chains: 10
Atoms: 18,183
Mol. weight: 296.34 kDa
Ligands: NAG
Released: 19 Feb 2020

Explore 6UE8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6UE8 contains 72 α-helices and 202 β-strands across 10 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	246-247	2	1
α-helix	250-252	3
α-helix	253-257	5
β-strand	265-269	5	1
β-strand	280-281	2	2
β-strand	290-291	2	1
β-strand	296	1	3
β-strand	302	1	3
β-strand	303-307	5	1
α-helix	313-316	4
β-strand	320-324	5	2
β-strand	335-339	5	2
β-strand	348-352	5	4
α-helix	353-355	3
β-strand	364-374	11	4
β-strand	380-385	6	5
β-strand	388-389	2	5
α-helix	390-391	2
α-helix	392-394	3
β-strand	396-397	2	4
β-strand	401-403	3	4
β-strand	410-420	11	4
α-helix	421-426	6
β-strand	430-435	6	5
β-strand	444-448	5	5
β-strand	457-463	7	6

Chain B: 9 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	245-249	5	7
α-helix	250-252	3
α-helix	253-257	5
β-strand	264-271	8	7
β-strand	280-281	2	8
β-strand	295	1	9
β-strand	302	1	7
β-strand	303	1	9
β-strand	304-308	5	7
α-helix	313-317	5
β-strand	321-324	4	8
β-strand	335-338	4	8
β-strand	348-352	5	10
α-helix	353-355	3
α-helix	358-361	4
β-strand	364-374	11	10
β-strand	380-385	6	11
β-strand	388-389	2	11
α-helix	390-391	2
α-helix	392-394	3
β-strand	395-397	3	10
β-strand	401-402	2	10
α-helix	403-405	3
β-strand	407	1	12
β-strand	411-413	3	10
β-strand	415-420	6	10
α-helix	423-426	4
β-strand	430-435	6	11
β-strand	444-448	5	11
β-strand	458-464	7	6

Chain C: 11 helices, 51 β-strands

Element	Residues	Length	Sheet
β-strand	5	1	13
β-strand	9-13	5	14
β-strand	19-23	5	13
α-helix	28-32	5
β-strand	35	1	15
β-strand	36-39	4	14
β-strand	48-51	4	14
β-strand	55	1	12
β-strand	56	1	14
β-strand	64-69	6	13
β-strand	74-79	6	13
β-strand	88-93	6	14
β-strand	94	1	15
β-strand	102-110	9	14
α-helix	111-113	3
β-strand	123-125	3	16
β-strand	130-131	2	17
β-strand	147	1	18
β-strand	149-151	3	19
β-strand	154-156	3	19
β-strand	174	1	17
β-strand	177	1	20
β-strand	185	1	20
β-strand	188-189	2	17
β-strand	202	1	18
β-strand	218-220	3	16
α-helix	221-224	4
β-strand	225-229	5	21
β-strand	234-239	6	22
α-helix	243-245	3
β-strand	250-254	5	21
β-strand	262-265	4	21
β-strand	271	1	21
α-helix	273-275	3
β-strand	279	1	22
α-helix	282-284	3
β-strand	290-295	6	22
α-helix	299-301	3
β-strand	304-309	6	21
β-strand	321-328	8	21
α-helix	335-336	2
β-strand	340-343	4	23
β-strand	348-354	7	24
α-helix	357-359	3
β-strand	365-366	2	23
β-strand	381-382	2	23
β-strand	387	1	23
β-strand	395-398	4	24
β-strand	405-411	7	24
β-strand	419-424	6	23
β-strand	433-439	7	23
β-strand	452	1	25
β-strand	460-462	3	26
α-helix	468-471	4
β-strand	474-481	8	27
β-strand	484-487	4	27
β-strand	500	1	26
β-strand	511-513	3	26
α-helix	518-520	3
β-strand	523-530	8	27
β-strand	533-541	9	27
β-strand	542	1	25

Chain D: 2 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	6-11	6	28
β-strand	16-23	8	28
β-strand	33-43	11	28
β-strand	47	1	29
β-strand	57	1	29
β-strand	60-63	4	6
α-helix	65-68	4
β-strand	75-78	4	30
β-strand	83-86	4	30
β-strand	87	1	31
α-helix	99-101	3
β-strand	111-114	4	11
β-strand	117	1	32
β-strand	122	1	32
β-strand	125-128	4	11

Chain E: 6 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	245-249	5	43
β-strand	265-269	5	43
β-strand	295-297	3	44
β-strand	301-303	3	44
β-strand	305-306	2	43
α-helix	313-315	3
β-strand	350-352	3	45
α-helix	353-355	3
α-helix	358-361	4
β-strand	364-372	9	45
β-strand	380-385	6	46
β-strand	388-389	2	46
α-helix	392-394	3
β-strand	396-397	2	45
β-strand	401-403	3	45
α-helix	404	1
β-strand	410-420	11	45
α-helix	422-426	5
β-strand	430-435	6	46
β-strand	458-463	6	28

Chain F: 9 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	245-247	3	33
α-helix	249-252	4
α-helix	254-257	4
β-strand	266-271	6	33
β-strand	279-280	2	34
β-strand	291	1	33
β-strand	302-306	5	33
α-helix	313-317	5
β-strand	321	1	35
β-strand	324-325	2	34
β-strand	334-335	2	34
β-strand	338	1	35
β-strand	348-352	5	36
α-helix	353-355	3
α-helix	356-359	4
β-strand	364-374	11	36
β-strand	380-385	6	31
β-strand	388-389	2	31
α-helix	390-391	2
α-helix	392-394	3
β-strand	395-397	3	36
β-strand	401-402	2	36
α-helix	403-404	2
β-strand	406	1	37
β-strand	408	1	37
β-strand	411-420	10	36
α-helix	421-426	6
β-strand	430-435	6	31
β-strand	443-448	6	31
β-strand	457-465	9	28

Chain G: 6 helices, 16 β-strands

Element	Residues	Length	Sheet
α-helix	253-256	4
β-strand	264	1	38
β-strand	290	1	39
β-strand	307	1	39
β-strand	308	1	38
β-strand	322-323	2	40
β-strand	336-337	2	40
β-strand	348-352	5	41
α-helix	353-355	3
α-helix	356-359	4
β-strand	364-374	11	41
β-strand	380-385	6	42
β-strand	388-389	2	42
α-helix	390-391	2
β-strand	396-397	2	41
β-strand	401-402	2	41
α-helix	403-404	2
β-strand	411-420	10	41
α-helix	421-426	6
β-strand	430-435	6	42
β-strand	443-448	6	42
β-strand	457-463	7	28

Chain H: 11 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	245-249	5	47
α-helix	250-252	3
α-helix	253-257	5
β-strand	265-269	5	47
β-strand	279	1	48
β-strand	290	1	47
β-strand	295-296	2	47
α-helix	298-300	3
β-strand	302-307	6	47
α-helix	312-315	4
β-strand	320-322	3	49
β-strand	325	1	48
β-strand	337-339	3	49
β-strand	348-352	5	50
α-helix	353-355	3
α-helix	358-361	4
β-strand	364-374	11	50
β-strand	380-385	6	51
β-strand	388-389	2	51
α-helix	390-391	2
α-helix	392-394	3
β-strand	396-397	2	50
β-strand	401-403	3	50
α-helix	404	1
β-strand	410-420	11	50
α-helix	422-426	5
β-strand	430-435	6	51
β-strand	443-448	6	51
α-helix	455-456	2
β-strand	457-463	7	28

2 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Immunoglobulin heavy constant alpha 2	A, B, E, F, G, H, K, L	protein	245	Homo sapiens	P01877 (AlphaFold model)
Polymeric immunoglobulin receptor	C	protein	591	Homo sapiens	P01833 (AlphaFold model)
Immunoglobulin J chain	D	protein	137	Homo sapiens	P01591 (AlphaFold model)

Sequence of entity 1 (A, B, E, F, G, H, K, L), FASTA

>6UE8_1 Immunoglobulin heavy constant alpha 2 (chains A, B, E, F, G, H, K, L)
DYKDDDDKLVPRGSCHPRLSLHRPALEDLLLGSEANLTCTLTGLRDASGATFTWTPSSGK
SAVQGPPERDLCGCYSVSSVLPGCAQPWNHGETFTCTAAHPELKTPLTANITKSGNTFRP
EVHLLPPPSEELALNELVTLTCLARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQG
TTTYAVTSILRVAAEDWKKGETFSCMVGHEALPLAFTQKTIDRLAGKPTHINVSVVMAEA
DGTCY

Sequence of entity 2 (C), FASTA

>6UE8_2 Polymeric immunoglobulin receptor (chains C)
KSPIFGPEEVNSVEGNSVSITCYYPPTSVNRHTRKYWCRQGARGGCITLISSEGYVSSKY
AGRANLTNFPENGTFVVNIAQLSQDDSGRYKCGLGINSRGLSFDVSLEVSQGPGLLNDTK
VYTVDLGRTVTINCPFKTENAQKRKSLYKQIGLYPVLVIDSSGYVNPNYTGRIRLDIQGT
GQLLFSVVINQLRLSDAGQYLCQAGDDSNSNKKNADLQVLKPEPELVYEDLRGSVTFHCA
LGPEVANVAKFLCRQSSGENCDVVVNTLGKRAPAFEGRILLNPQDKDGSFSVVITGLRKE
DAGRYLCGAHSDGQLQEGSPIQAWQLFVNEESTIPRSPTVVKGVAGGSVAVLCPYNRKES
KSIKYWCLWEGAQNGRCPLLVDSEGWVKAQYEGRLSLLEEPGNGTFTVILNQLTSRDAGF
YWCLTNGDTLWRTTVEIKIIEGEPNLKVPGNVTAVLGETLKVPCHFPCKFSSYEKYWCKW
NNTGCQALPSQDEGPSKAFVNCDENSRLVSLTLNLVTRADEGWYWCGVKQGHFYGETAAV
YVAVEERKAAGSRDVSLAKADAAPDEKVLDSGFREIENKAIQDPRHHHHHH

Sequence of entity 3 (D), FASTA

>6UE8_3 Immunoglobulin J chain (chains D)
QEDERIVLVDNKCKCARITSRIIRSSEDPNEDIVERNIRIIVPLNNRENISDPTSPLRTR
FVYHLSDLCKKCDPTEVELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGG
ETKMVETALTPDACYPD

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	5

Primary citation

Structure of the secretory immunoglobulin A core. Kumar, N., Arthur, C.P., Ciferri, C. et al. Science (2020) 367:1008-1014. DOI 10.1126/science.aaz5807 · PubMed

Other PDB entries of the same protein (UniProt P01877 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9OFS 1.95 Å, Crystal structure of the human IGA2m2 FC fragment-FC-alpha receptor (CD89) complex
6UE9 2.9 Å, Structure of tetrameric sIgA complex (Class 2)
6UEA 3.0 Å, Structure of pentameric sIgA complex
3CM9 Solution Structure of Human SIgA2

Browse structure collections

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