6UEA: Pentameric sIgA complex

Structure of pentameric sIgA complex. Determined by electron microscopy at 3.0 Å resolution. Released 19 Feb 2020.

Method: Electron microscopy
Resolution: 3.0 Å
Organism: Homo sapiens
Chains: 12
Atoms: 21,299
Mol. weight: 351.49 kDa
Ligands: NAG
Released: 19 Feb 2020

Explore 6UEA in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6UEA contains 75 α-helices and 265 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	245	1	1
β-strand	248-249	2	2
α-helix	250-252	3
α-helix	253-257	5
β-strand	263-266	4	2
β-strand	268-269	2	1
β-strand	280-281	2	3
β-strand	290	1	2
β-strand	295-296	2	1
β-strand	302-304	3	1
β-strand	307-309	3	2
α-helix	313-317	5
β-strand	321-322	2	4
β-strand	323-324	2	3
β-strand	337-338	2	4
β-strand	348-352	5	5
α-helix	353-355	3
β-strand	364-374	11	5
β-strand	380-385	6	6
β-strand	388-389	2	6
α-helix	390-391	2
α-helix	392-394	3
β-strand	396-397	2	5
β-strand	401-403	3	5
β-strand	410-420	11	5
α-helix	421-425	5
β-strand	430-435	6	6
β-strand	444-448	5	6
β-strand	457-464	8	7

Chain B: 9 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	245-249	5	8
α-helix	250-252	3
α-helix	254-257	4
β-strand	264-271	8	8
β-strand	278-279	2	9
β-strand	295-296	2	8
β-strand	302-308	7	8
α-helix	312-317	6
β-strand	320-323	4	10
β-strand	325-326	2	9
β-strand	336-339	4	10
β-strand	348-352	5	11
α-helix	353-355	3
α-helix	358-361	4
β-strand	364-372	9	11
β-strand	380-385	6	12
β-strand	388-389	2	12
α-helix	390-391	2
α-helix	392-394	3
β-strand	395-397	3	11
β-strand	401-402	2	11
α-helix	403-405	3
β-strand	407	1	13
β-strand	411-413	3	11
β-strand	415-420	6	11
α-helix	421-426	6
β-strand	430-435	6	12
β-strand	444-448	5	12
β-strand	449	1	14
β-strand	452	1	14
β-strand	458-464	7	7

Chain C: 8 helices, 58 β-strands

Element	Residues	Length	Sheet
β-strand	5	1	15
β-strand	9-13	5	16
β-strand	18-23	6	15
α-helix	28-32	5
β-strand	35	1	17
β-strand	36-39	4	16
β-strand	48-51	4	16
β-strand	55	1	13
β-strand	56	1	16
β-strand	65-69	5	15
β-strand	74-79	6	15
β-strand	88-93	6	16
β-strand	94	1	17
β-strand	102-110	9	16
β-strand	122-124	3	18
β-strand	130-131	2	19
β-strand	145	1	20
β-strand	147-151	5	21
β-strand	154-159	6	21
β-strand	173	1	19
β-strand	176-177	2	22
β-strand	185-186	2	22
β-strand	188-189	2	19
β-strand	200-202	3	23
β-strand	204	1	20
β-strand	213-215	3	23
β-strand	217-219	3	18
α-helix	224	1
β-strand	225-229	5	24
β-strand	234-239	6	25
β-strand	249-254	6	24
β-strand	262-266	5	24
β-strand	271	1	24
β-strand	279	1	25
β-strand	290-295	6	25
β-strand	304-310	7	24
β-strand	324-328	5	24
α-helix	334-336	3
β-strand	340-341	2	26
β-strand	344	1	27
β-strand	349-354	6	28
α-helix	357-359	3
β-strand	364-368	5	26
β-strand	379-382	4	26
β-strand	387	1	26
α-helix	389-391	3
β-strand	395-401	7	28
β-strand	405-410	6	28
α-helix	415-417	3
β-strand	420-425	6	26
β-strand	434-437	4	26
β-strand	440	1	27
β-strand	447	1	29
β-strand	452	1	30
β-strand	461	1	31
β-strand	465	1	29
α-helix	468-470	3
β-strand	474-480	7	32
β-strand	481	1	33
β-strand	484	1	33
β-strand	512	1	31
α-helix	518-520	3
β-strand	523-530	8	32
β-strand	533-534	2	32
β-strand	538-541	4	32
β-strand	542	1	30

Chain D: 4 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	6-11	6	34
β-strand	16-23	8	34
β-strand	33-43	11	34
β-strand	47	1	35
α-helix	56	1
β-strand	57	1	35
α-helix	58	1
β-strand	60-63	4	7
α-helix	65-68	4
β-strand	75-77	3	36
β-strand	84-86	3	36
β-strand	87	1	37
α-helix	100-101	2
β-strand	111-114	4	12
β-strand	117	1	38
β-strand	122	1	38
β-strand	125-128	4	12

Chain E: 6 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	245-247	3	61
α-helix	254-257	4
β-strand	264	1	62
β-strand	267-269	3	61
β-strand	308	1	62
α-helix	313-316	4
α-helix	333-334	2
β-strand	348-351	4	63
α-helix	353-355	3
β-strand	364-372	9	63
β-strand	380-385	6	64
β-strand	388-389	2	64
β-strand	396-397	2	63
α-helix	398-400	3
β-strand	401-403	3	63
β-strand	410-420	11	63
α-helix	421-426	6
β-strand	430-435	6	64
β-strand	444-445	2	64
β-strand	458	1	34
β-strand	461-463	3	34

Chain F: 8 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	246-249	4	39
α-helix	254-257	4
β-strand	264-268	5	39
β-strand	269	1	40
β-strand	278-280	3	41
β-strand	291	1	39
β-strand	303	1	40
β-strand	306-308	3	39
α-helix	314-317	4
β-strand	320-321	2	42
β-strand	324-326	3	41
β-strand	335	1	41
β-strand	338-339	2	42
β-strand	348-352	5	43
α-helix	353-355	3
α-helix	358-361	4
β-strand	364-372	9	43
β-strand	381-385	5	37
β-strand	388-389	2	37
α-helix	390-391	2
α-helix	392-394	3
β-strand	395-397	3	43
β-strand	401-402	2	43
α-helix	403-404	2
β-strand	411-420	10	43
α-helix	421-426	6
β-strand	430-434	5	37
β-strand	444-448	5	37
β-strand	457-465	9	34

Chain G: 5 helices, 18 β-strands

Element	Residues	Length	Sheet
β-strand	245-246	2	44
β-strand	268-269	2	44
β-strand	278-281	4	45
β-strand	290-291	2	46
β-strand	303-304	2	44
β-strand	306-307	2	46
β-strand	323-326	4	45
β-strand	335-336	2	45
β-strand	348-352	5	47
α-helix	353-355	3
α-helix	356-359	4
β-strand	364-374	11	47
β-strand	380-385	6	48
β-strand	388-389	2	48
α-helix	390-391	2
β-strand	396-397	2	47
β-strand	401-402	2	47
α-helix	403-404	2
β-strand	411-420	10	47
α-helix	421-426	6
β-strand	430-435	6	48
β-strand	443-448	6	48
β-strand	457-463	7	34

Chain H: 6 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	247-248	2	65
α-helix	250-252	3
α-helix	253-255	3
β-strand	264-265	2	66
β-strand	266-267	2	65
β-strand	268	1	67
β-strand	281	1	68
β-strand	291	1	69
β-strand	304	1	67
β-strand	306	1	69
β-strand	307-308	2	66
α-helix	313-316	4
β-strand	323	1	68
β-strand	324	1	70
β-strand	335	1	70
α-helix	346-347	2
β-strand	348-352	5	71
α-helix	353-355	3
β-strand	364-372	9	71
β-strand	380-384	5	72
β-strand	389	1	72
β-strand	396-397	2	71
β-strand	401-402	2	71
β-strand	411-420	10	71
α-helix	421-426	6
β-strand	430-435	6	72
β-strand	443-448	6	72
β-strand	457-463	7	34

4 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Immunoglobulin heavy constant alpha 2	A, B, E, F, G, H, I, J, K, L	protein	245	Homo sapiens	P01877 (AlphaFold model)
Polymeric immunoglobulin receptor	C	protein	591	Homo sapiens	P01833 (AlphaFold model)
Immunoglobulin J chain	D	protein	137	Homo sapiens	P01591 (AlphaFold model)

Sequence of entity 1 (A, B, E, F, G, H, I, J, K, L), FASTA

>6UEA_1 Immunoglobulin heavy constant alpha 2 (chains A, B, E, F, G, H, I, J, K, L)
DYKDDDDKLVPRGSCHPRLSLHRPALEDLLLGSEANLTCTLTGLRDASGATFTWTPSSGK
SAVQGPPERDLCGCYSVSSVLPGCAQPWNHGETFTCTAAHPELKTPLTANITKSGNTFRP
EVHLLPPPSEELALNELVTLTCLARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQG
TTTYAVTSILRVAAEDWKKGETFSCMVGHEALPLAFTQKTIDRLAGKPTHINVSVVMAEA
DGTCY

Sequence of entity 2 (C), FASTA

>6UEA_2 Polymeric immunoglobulin receptor (chains C)
KSPIFGPEEVNSVEGNSVSITCYYPPTSVNRHTRKYWCRQGARGGCITLISSEGYVSSKY
AGRANLTNFPENGTFVVNIAQLSQDDSGRYKCGLGINSRGLSFDVSLEVSQGPGLLNDTK
VYTVDLGRTVTINCPFKTENAQKRKSLYKQIGLYPVLVIDSSGYVNPNYTGRIRLDIQGT
GQLLFSVVINQLRLSDAGQYLCQAGDDSNSNKKNADLQVLKPEPELVYEDLRGSVTFHCA
LGPEVANVAKFLCRQSSGENCDVVVNTLGKRAPAFEGRILLNPQDKDGSFSVVITGLRKE
DAGRYLCGAHSDGQLQEGSPIQAWQLFVNEESTIPRSPTVVKGVAGGSVAVLCPYNRKES
KSIKYWCLWEGAQNGRCPLLVDSEGWVKAQYEGRLSLLEEPGNGTFTVILNQLTSRDAGF
YWCLTNGDTLWRTTVEIKIIEGEPNLKVPGNVTAVLGETLKVPCHFPCKFSSYEKYWCKW
NNTGCQALPSQDEGPSKAFVNCDENSRLVSLTLNLVTRADEGWYWCGVKQGHFYGETAAV
YVAVEERKAAGSRDVSLAKADAAPDEKVLDSGFREIENKAIQDPRHHHHHH

Sequence of entity 3 (D), FASTA

>6UEA_3 Immunoglobulin J chain (chains D)
QEDERIVLVDNKCKCARITSRIIRSSEDPNEDIVERNIRIIVPLNNRENISDPTSPLRTR
FVYHLSDLCKKCDPTEVELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGG
ETKMVETALTPDACYPD

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	2

Primary citation

Structure of the secretory immunoglobulin A core. Kumar, N., Arthur, C.P., Ciferri, C. et al. Science (2020) 367:1008-1014. DOI 10.1126/science.aaz5807 · PubMed

Other PDB entries of the same protein (UniProt P01877 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9OFS 1.95 Å, Crystal structure of the human IGA2m2 FC fragment-FC-alpha receptor (CD89) complex
6UE9 2.9 Å, Structure of tetrameric sIgA complex (Class 2)
6UE8 3.0 Å, Structure of tetrameric sIgA complex (Class 1)
3CM9 Solution Structure of Human SIgA2

Browse structure collections

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