6YTP: Recombinant human beta-glucocerebrosidase

Structure of recombinant human beta-glucocerebrosidase in complex with azide tagged cyclophellitol epoxide inhibitor. Determined by X-ray diffraction at 1.7 Å resolution. Released 12 May 2021.

Method: X-ray diffraction
Resolution: 1.7 Å
Organism: Homo sapiens
Chains: 2
Atoms: 8,899
Mol. weight: 115.81 kDa
Ligands: NAG, PN8
Released: 12 May 2021

Explore 6YTP in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6YTP contains 49 α-helices and 55 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain AAA: 25 helices, 28 β-strands

Element	Residues	Length	Sheet
β-strand	2	1	1
β-strand	6-7	2	2
α-helix	14	1
β-strand	15-18	4	2
β-strand	25	1	1
α-helix	26-29	4
α-helix	31-33	3
β-strand	36-43	8	3
β-strand	50-55	6	3
α-helix	56	1
β-strand	57	1	3
β-strand	65-77	13	3
α-helix	78	1
β-strand	80-84	5	4
α-helix	87-94	8
α-helix	98-109	12
β-strand	118-123	6	4
α-helix	151	1
α-helix	152-157	6
α-helix	158-167	10
α-helix	171-172	2
β-strand	173-178	6	4
α-helix	183-185	3
β-strand	186	1	5
β-strand	197	1	5
α-helix	204-222	19
β-strand	229-231	3	4
α-helix	238-240	3
α-helix	253-259	7
α-helix	260-264	5
α-helix	265-269	5
β-strand	277-284	8	4
α-helix	285-287	3
α-helix	290-296	7
α-helix	299-302	4
β-strand	307-313	7	4
α-helix	321-325	5
α-helix	326-330	5
β-strand	335-342	8	4
α-helix	357-372	16
β-strand	375	1	4
β-strand	377-382	6	4
β-strand	385	1	2
β-strand	402-405	4	2
α-helix	406-408	3
β-strand	410-413	4	2
α-helix	415-424	10
β-strand	432-438	7	3
β-strand	444-450	7	3
β-strand	456-462	7	3
β-strand	468-474	7	3
β-strand	478-484	7	3
β-strand	488-494	7	3

Chain BBB: 24 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	2	1	6
β-strand	6-7	2	7
β-strand	15-19	5	7
β-strand	25	1	6
α-helix	26-29	4
β-strand	36-43	8	8
β-strand	50-55	6	8
β-strand	57	1	8
β-strand	65-77	13	8
α-helix	78	1
β-strand	80-84	5	9
α-helix	87-94	8
α-helix	98-109	12
β-strand	118-123	6	9
α-helix	151	1
α-helix	152-157	6
α-helix	158-167	10
α-helix	171-172	2
β-strand	173-178	6	9
α-helix	183-185	3
β-strand	186	1	10
β-strand	197	1	10
α-helix	204-222	19
β-strand	229-231	3	9
α-helix	238-240	3
α-helix	253-259	7
α-helix	260-264	5
α-helix	265-269	5
β-strand	277-284	8	9
α-helix	285-287	3
α-helix	290-296	7
α-helix	299-302	4
β-strand	307-311	5	9
α-helix	315-317	3
α-helix	320	1
α-helix	321-325	5
α-helix	326-330	5
β-strand	335-340	6	9
α-helix	357-372	16
β-strand	375-382	8	9
β-strand	385	1	7
β-strand	402-405	4	7
α-helix	406-408	3
β-strand	410-413	4	7
α-helix	415-424	10
β-strand	432-438	7	8
β-strand	444-450	7	8
β-strand	456-462	7	8
β-strand	468-474	7	8
β-strand	478-484	7	8
β-strand	488-494	7	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Glucosylceramidase	AAA, BBB	protein	497	Homo sapiens	P04062 (AlphaFold model)

Sequence of entity 1 (AAA, BBB), FASTA

>6YTP_1 Glucosylceramidase (chains AAA, BBB)
ARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANH
TGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIR
VPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWT
SPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGL
LSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPE
AAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRG
MQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHL
GHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFL
ETISPGYSIHTYLWHRQ

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	2
PN8	(1~{S},2~{R},3~{R},4~{S},5~{S})-4-[[($l^{5}-azanylidyne-$l^{5}-azanyl)amino]met…	C7 H14 N3 O4	2

Water and common crystallization additives (SO4, EDO, GOL) are not listed.

Primary citation

Design, Synthesis and Structural Analysis of Glucocerebrosidase Imaging Agents. Rowland, R.J., Chen, Y., Breen, I. et al. Chemistry (2021) 27:16377-16388. DOI 10.1002/chem.202102359 · PubMed

Other PDB entries of the same protein (UniProt P04062 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6TN1 0.98 Å, Unliganded Crystal Structure of Recombinant GBA
9FB2 1.14 Å, Gcase in complex with small molecule inhibitor 1
9FA3 1.36 Å, Gcase in complex with small molecule inhibitor 1
9FAL 1.39 Å, Gcase in complex with small molecule inhibitor 1
9FAY 1.4 Å, Gcase in complex with small molecule inhibitor 1
6TJQ 1.41 Å, Crystal Structure of Recombinant GBA in Complex with 2-Deoxy-2-fluoro-beta-D-glucopyranos…
9FDI 1.41 Å, Gcase in complex with small molecule inhibitor 1
8AWR 1.49 Å, Structure of recombinant human beta-glucocerebrosidase in complex with L-carbaxylosyl…
9FA6 1.49 Å, Gcase in complex with small molecule inhibitor 1
6TJK 1.56 Å, Crystal Structure of Recombinant GBA in Complex with Bis-Tris Propane.
8AWK 1.58 Å, Structure of recombinant human beta-glucocerebrosidase in complex with D-carbaxylosyl…
6TJJ 1.59 Å, Structure of Cerezyme at pH 4.6

Browse structure collections

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