6ZJN: NADH-quinone oxidoreductase subunit 1

Respiratory complex I from Thermus thermophilus, NADH dataset, minor state. Determined by electron microscopy at 6.1 Å resolution. Released 2 Sept 2020.

Method: Electron microscopy
Resolution: 6.1 Å
Organism: Thermus thermophilus
Chains: 15
Atoms: 22,855
Mol. weight: 525.63 kDa
Ligands: FES, SF4
Released: 2 Sept 2020

Explore 6ZJN in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6ZJN contains 223 α-helices and 137 β-strands across 15 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 1: 26 helices, 16 β-strands

Element	Residues	Length	Sheet
β-strand	21	1	1
β-strand	23	1	1
α-helix	29-33	5
α-helix	39-46	8
α-helix	50-60	11
α-helix	72-77	6
β-strand	88-93	6	2
α-helix	102-109	8
α-helix	111-124	14
β-strand	130-134	5	2
α-helix	139-154	16
β-strand	171-175	5	2
α-helix	180-183	4
α-helix	185-193	9
α-helix	196-197	2
α-helix	211-213	3
β-strand	216-220	5	2
α-helix	221-225	5
α-helix	227-232	6
α-helix	235-239	5
β-strand	249-254	6	3
β-strand	262-267	6	3
β-strand	271	1	4
α-helix	272-274	3
α-helix	275-279	5
β-strand	287-291	5	5
α-helix	298-299	2
β-strand	300-301	2	5
α-helix	304-307	4
β-strand	310	1	4
α-helix	311	1
β-strand	327	1	3
β-strand	329-332	4	5
α-helix	337-350	14
α-helix	357	1
α-helix	358-367	10
α-helix	368-374	7
β-strand	376	1	6
α-helix	381-395	15
α-helix	402-416	15
α-helix	418-426	9
β-strand	430	1	6

Chain 2: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	8-16	9
α-helix	23-26	4
α-helix	27-38	12
α-helix	43-53	11
α-helix	57-64	8
β-strand	78-83	6	7
α-helix	86-88	3
α-helix	89-93	5
α-helix	94-104	11
β-strand	111	1	7
β-strand	117-122	6	7
β-strand	133-136	4	7
β-strand	139-143	5	7
α-helix	147-158	12
α-helix	163-165	3
β-strand	176	1	3

Chain 3: 35 helices, 42 β-strands

Element	Residues	Length	Sheet
β-strand	4-6	3	8
β-strand	9-11	3	8
β-strand	18	1	9
α-helix	19-26	8
β-strand	49-50	2	10
β-strand	51-53	3	8
β-strand	75	1	8
β-strand	80-81	2	10
β-strand	86	1	9
α-helix	87	1
β-strand	91-94	4	8
α-helix	98-112	15
α-helix	125-127	3
α-helix	129-137	9
β-strand	168-169	2	11
β-strand	174-176	3	11
α-helix	178-180	3
α-helix	186-193	8
β-strand	201-202	2	12
β-strand	211-212	2	12
α-helix	225-229	5
β-strand	235-237	3	11
α-helix	238-240	3
α-helix	246-248	3
β-strand	250-256	7	13
β-strand	263-270	8	13
β-strand	273-279	7	13
α-helix	292-296	5
α-helix	300-302	3
β-strand	310-312	3	14
β-strand	317-319	3	14
α-helix	322-332	11
β-strand	343-345	3	14
α-helix	351-363	13
β-strand	368	1	15
β-strand	369-371	3	14
α-helix	379-381	3
β-strand	395-399	5	16
α-helix	402-405	4
α-helix	409-417	9
β-strand	448-452	5	16
α-helix	457-461	5
β-strand	464-467	4	16
α-helix	468-469	2
α-helix	473-478	6
α-helix	479-483	5
α-helix	488-499	12
β-strand	503-507	5	16
α-helix	509-512	4
α-helix	515-527	13
β-strand	532-534	3	16
α-helix	541-546	6
β-strand	551	1	15
β-strand	565-568	4	14
α-helix	574-577	4
β-strand	582-587	6	14
α-helix	592-595	4
β-strand	600-603	4	14
α-helix	607-609	3
β-strand	612-615	4	17
β-strand	620	1	18
β-strand	621-624	4	17
α-helix	637-646	10
α-helix	650-652	3
α-helix	657-666	10
β-strand	675	1	18
β-strand	692-696	5	19
α-helix	701-703	3
α-helix	708-711	4
β-strand	714-718	5	19
α-helix	720-725	6
β-strand	734-738	5	19
β-strand	741-749	9	19
α-helix	754	1
β-strand	758-762	5	19
β-strand	770-771	2	19
β-strand	774	1	19

Chain 4: 20 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	27-30	4	20
β-strand	42-47	6	20
β-strand	51	1	21
β-strand	52-58	7	20
α-helix	66-70	5
α-helix	75-78	4
α-helix	82-84	3
α-helix	91-105	15
α-helix	111-139	29
α-helix	145-164	20
β-strand	173	1	22
β-strand	178	1	22
α-helix	181-183	3
α-helix	186-209	24
α-helix	212-218	7
α-helix	226-231	6
α-helix	239-242	4
α-helix	248-251	4
α-helix	257-259	3
α-helix	272-295	24
α-helix	311-313	3
α-helix	321-332	12
β-strand	339-342	4	23
β-strand	345-348	4	23
β-strand	351-360	10	23
β-strand	365-371	7	23
α-helix	373-376	4
α-helix	381-384	4
β-strand	389	1	21
α-helix	392-400	9
α-helix	404-408	5

Chain 5: 5 helices, 12 β-strands

Element	Residues	Length	Sheet
α-helix	4-12	9
β-strand	17-20	4	24
β-strand	24-27	4	24
β-strand	28	1	25
α-helix	34-43	10
β-strand	48-56	9	25
β-strand	68-75	8	25
β-strand	88-94	7	25
β-strand	100-102	3	26
α-helix	111-122	12
α-helix	124	1
β-strand	125-127	3	26
β-strand	157-159	3	27
β-strand	163-165	3	27
α-helix	167-169	3
β-strand	189	1	28
β-strand	191	1	28

Chain 6: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	22-34	13
β-strand	38-42	5	29
α-helix	46-54	9
α-helix	61-63	3
β-strand	77-81	5	29
α-helix	89-97	9
β-strand	104-108	5	29
α-helix	109-113	5
α-helix	127-129	3
β-strand	135-137	3	29
α-helix	144-156	13
α-helix	157-161	5
β-strand	163	1	30
β-strand	169	1	30

Chain 7: 5 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	5-27	23
β-strand	31-36	6	35
α-helix	38-41	4
β-strand	54-60	7	35
β-strand	66-72	7	35
α-helix	73-74	2
β-strand	82-86	5	35
β-strand	91-95	5	35
β-strand	96-98	3	36
β-strand	102-105	4	36
β-strand	108-109	2	36
α-helix	110	1
α-helix	112-126	15

Chain 9: 9 helices, 10 β-strands

Element	Residues	Length	Sheet
α-helix	4-18	15
α-helix	30	1
β-strand	31-32	2	31
β-strand	42-43	2	32
α-helix	58-62	5
β-strand	68-73	6	33
β-strand	87-94	8	33
α-helix	103-107	5
β-strand	113-114	2	32
α-helix	126-128	3
β-strand	130-132	3	33
α-helix	133-135	3
β-strand	137	1	32
α-helix	144-152	9
β-strand	161-162	2	31
β-strand	163	1	34
α-helix	175-176	2
β-strand	177	1	34
α-helix	178	1

7 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
NADH-quinone oxidoreductase subunit 1	1	protein	438	Thermus thermophilus	Q56222 (AlphaFold model)
NADH-quinone oxidoreductase subunit 2	2	protein	181	Thermus thermophilus	Q56221 (AlphaFold model)
NADH-quinone oxidoreductase subunit 3	3	protein	783	Thermus thermophilus	Q56223 (AlphaFold model)
NADH-quinone oxidoreductase subunit 4	4	protein	409	Thermus thermophilus	Q56220 (AlphaFold model)
NADH-quinone oxidoreductase subunit 5	5	protein	207	Thermus thermophilus	Q56219
NADH-quinone oxidoreductase subunit 6	6	protein	181	Thermus thermophilus	Q56218
NADH-quinone oxidoreductase subunit 9	9	protein	182	Thermus thermophilus	Q56224
NADH-quinone oxidoreductase subunit 15	7	protein	129	Thermus thermophilus	Q5SKZ7
NADH-quinone oxidoreductase subunit 7	A	protein	119	Thermus thermophilus	Q56217
NADH-quinone oxidoreductase subunit 10	J	protein	176	Thermus thermophilus	Q56225
NADH-quinone oxidoreductase subunit 11	K	protein	95	Thermus thermophilus	Q56226
NADH-quinone oxidoreductase subunit 12	L	protein	606	Thermus thermophilus	Q56227

3 more molecules are not listed.

Sequence of entity 1 (1), FASTA

>6ZJN_1 NADH-quinone oxidoreductase subunit 1 (chains 1)
MTGPILSGLDPRFERTLYAHVGKEGSWTLDYYLRHGGYETAKRVLKEKTPDEVIEEVKRS
GLRGRGGAGFPTGLKWSFMPKDDGKQHYLICNADESEPGSFKDRYILEDVPHLLIEGMIL
AGYAIRATVGYIYVRGEYRRAADRLEQAIKEARARGYLGKNLFGTDFSFDLHVHRGAGAY
ICGEETALMNSLEGLRANPRLKPPFPAQSGLWGKPTTINNVETLASVVPIMERGADWFAQ
MGTEQSKGMKLYQISGPVKRPGVYELPMGTTFRELIYEWAGGPLEPIQAIIPGGSSTPPL
PFTEEVLDTPMSYEHLQAKGSMLGTGGVILIPERVSMVDAMWNLTRFYAHESCGKCTPCR
EGVAGFMVNLFAKIGTGQGEEKDVENLEALLPLIEGRSFCPLADAAVWPVKGSLRHFKDQ
YLALAREKRPVPRPSLWR

Sequence of entity 2 (2), FASTA

>6ZJN_2 NADH-quinone oxidoreductase subunit 2 (chains 2)
MGFFDDKQDFLEETFAKYPPEGRRAAIMPLLRRVQQEEGWIRPERIEEIARLVGTTPTEV
MGVASFYSYYQFVPTGKYHLQVCATLSCKLAGAEELWDYLTETLGIGPGEVTPDGLFSVQ
KVECLGSCHTAPVIQVNDEPYVECVTRARLEALLAGLRAGKRLEEIELPGKCGHHVHEVE
V

Sequence of entity 3 (3), FASTA

>6ZJN_3 NADH-quinone oxidoreductase subunit 3 (chains 3)
MVRVKVNDRIVEVPPGTSVMDAVFHAGYDVPLFCSEKHLSPIGACRMCLVRIGLPKKGPD
GKPLLNEKGEPEIQWQPKLAASCVTAVADGMVVDTLSDVVREAQAGMVEFTLLNHPLDCP
TCDKGGACELQDRTVEYGLYEKYYQKGPLELPVYTRFEFTRRHVDKHHPLSPFVILDRER
CIHCKRCVRYFEEVPGDEVLDFIERGVHTFIGTMDFGLPSGFSGNITDICPVGALLDLTA
RFRARNWEMEETPTTCALCPVGCGITADTRSGELLRIRAREVPEVNEIWICDAGRFGHEW
ADQNRLKTPLVRKEGRLVEATWEEAFLALKEGLKEARGEEVGLYLAHDATLEEGLLASEL
AKALKTPHLDFQGRTAAPASLFPPASLEDLLQADFALVLGDPTEEAPILHLRLSEFVRDL
KPPHRYNHGTPFADLQIKERMPRRTDKMALFAPYRAPLMKWAAIHEVHRPGEEREILLAL
LGDKEGSEMVAKAKEAWEKAKNPVLILGAGVLQDTVAAERARLLAERKGAKVLAMTPAAN
ARGLEAMGVLPGAKGASWDEPGALYAYYGFVPPEEALKGKRFVVMHLSHLHPLAERYAHV
VLPAPTFYEKRGHLVNLEGRVLPLSPAPIENGEAEGALQVLALLAEALGVRPPFRLHLEA
QKALKARKVPEAMGRLSFRLKELRPKERKGAFYLRPTMWKAHQAVGKAQEAARAELWAHP
ETARAEALPEGAQVAVETPFGRVEARVVHREDVPKGHLYLSALGPAAGLRVEGRVLVPAG
GEA

Sequence of entity 4 (4), FASTA

>6ZJN_4 NADH-quinone oxidoreductase subunit 4 (chains 4)
MREEFLEEIPLDAPPEEAKELRTEVMTLNVGPQHPSTHGVLRLMVTLSGEEVLEVVPHIG
YLHTGFEKTMEHRTYLQNITYTPRMDYLHSFAHDLAYALAVEKLLGAVVPPRAETIRVIL
NELSRLASHLVFLGTGLLDLGALTPFFYAFRERETILDLFEWVTGQRFHHNYIRIGGVKE
DLPEEFVPELKKLLEVLPHRIDEYEALFAESPIFYERARGVGVIPPEVAIDLGLTGGSLR
ASGVNYDVRKAYPYSGYETYTFDVPLGERGDVFDRMLVRIREMRESVKIIKQALERLEPG
PVRDPNPQITPPPRHLLETSMEAVIYHFKHYTEGFHPPKGEVYVPTESARGELGYYIVSD
GGSMPYRVKVRAPSFVNLQSLPYACKGEQVPDMVAIIASLDPVMGDVDR

Sequence of entity 5 (5), FASTA

>6ZJN_5 NADH-quinone oxidoreductase subunit 5 (chains 5)
MRLERVLEEARAKGYPIEDNGLGNLWVVLPRERFKEEMAHYKAMGFNFLADIVGLDYLTY
PDPRPERFAVVYELVSLPGWKDGDGSRFFVRVYVPEEDPRLPTVTDLWGSANFLEREVYD
LFGIVFEGHPDLRKILTPEDLEGHPLRKDYPLGETPTLFREGRYIIPAEFRAALTGKDPG
LTFYKGGSRKGYRSLWADLKKAREVKG

Sequence of entity 6 (6), FASTA

>6ZJN_6 NADH-quinone oxidoreductase subunit 6 (chains 6)
MALKDLFERDVQELEREGILFTTLEKLVAWGRSNSLWPATFGLACCAIEMMASTDARNDL
ARFGSEVFRASPRQADVMIVAGRLSKKMAPVMRRVWEQMPDPKWVISMGACASSGGMFNN
YAIVQNVDSVVPVDVYVPGCPPRPEALIYAVMQLQKKVRGQAYNERGERLPPVAAWKRTR
G

Sequence of entity 7 (9), FASTA

>6ZJN_7 NADH-quinone oxidoreductase subunit 9 (chains 9)
MTLKALAQSLGITLKYLFSKPVTVPYPDAPVALKPRFHGRHVLTRHPNGLEKCIGCSLCA
AACPAYAIYVEPAENDPENPVSAGERYAKVYEINMLRCIFCGLCEEACPTGAIVLGYDFE
MADYEYSDLVYGKEDMLVDVVGTKPQRREAKRTGKPVKVGYVVPYVRPELEGFKAPTEGG
KR

Sequence of entity 8 (7), FASTA

>6ZJN_8 NADH-quinone oxidoreductase subunit 15 (chains 7)
MSASSERELYEAWVELLSWMREYAQAKGVRFEKEADFPDFIYRMERPYDLPTTIMTASLS
DGLGEPFLLADVSPRHAKLKRIGLRLPRAHIHLHAHYEPGKGLVTGKIPLTKERFFALAD
RAREALAFA

Sequence of entity 9 (A), FASTA

>6ZJN_9 NADH-quinone oxidoreductase subunit 7 (chains A)
MAPIQEYVGTLIYVGVALFIGVAALLVGALLGPKKPGRAKLMPYESGNDPAGEVKRFPVH
FYVVAMLFILFDVEVAFLWPYAVSAGGLGLYGFLGVLAFTLLLFVGFLYEWWKGVMRWH

Sequence of entity 10 (J), FASTA

>6ZJN_10 NADH-quinone oxidoreductase subunit 10 (chains J)
MSLLEGLALFLLLLSGVLVVTLRNAIHAALALILNFLVLAGVYVALDARFLGFIQVIVYA
GAIVVLFLFVIMLLFAAQGEIGFDPLVRSRPLAALLALGVAGILAAGLWGLDLAFTQDLK
GGLPQALGPLLYGDWLFVLLAVGFLLMAATVVAVALVEPGKASRAKEAEKREEVAR

Sequence of entity 11 (K), FASTA

>6ZJN_11 NADH-quinone oxidoreductase subunit 11 (chains K)
MSYLLTSALLFALGVYGVLTRRTAILVFLSIELMLNAANLSLVGFARAYGLDGQVAALMV
IAVAAAEVAVGLGLIVAIFRHRESTAVDDLSELRG

Sequence of entity 12 (L), FASTA

>6ZJN_12 NADH-quinone oxidoreductase subunit 12 (chains L)
MALLGTILLPLLGFALLGLFGKRMREPLPGVLASGLVLASFLLGAGLLLSGGARFQAEWL
PGIPFSLLLDNLSGFMLLIVTGVGFLIHVYAIGYMGGDPGYSRFFAYFNLFIAMMLTLVL
ADSYPVMFIGWEGVGLASFLLIGFWYKNPQYADSARKAFIVNRIGDLGFMLGMAILWALY
GTLSISELKEAMEGPLKNPDLLALAGLLLFLGAVGKSAQIPLMVWLPDAMAGPTPVSALI
HAATMVTAGVYLIARSSFLYSVLPDVSYAIAVVGLLTAAYGALSAFGQTDIKKIVAYSTI
SQLGYMFLAAGVGAYWVALFHVFTHAFFKALLFLASGSVIHALGGEQDVRKMGGLWKHLP
QTRWHALIGALALGGLPLLSGFWSKDAILAATLTYPFGGVGFYVGALLVAVLTAMYAMRW
FVLVFLGEERGHHHPHEAPPVMLWPNHLLALGSVLAGYLALPHPLPNVLEPFLKPALAEV
EAHHLSLGAEWGLIALSAAVALLGLWAGFVFFQRKVFPAWYLAFEAASREAFYVDRAYNA
LIVNPLKALAEALFYGDRGLLSGYFGLGGAARSLGQGLARLQTGYLRVYALLFVLGALLL
LGVMRW

Ligands and cofactors

ID	Name	Formula	Copies
FES	FE2/S2 (inorganic) cluster	Fe2 S2	2
SF4	Iron/sulfur cluster	Fe4 S4	7

Primary citation

Key role of quinone in the mechanism of respiratory complex I. Gutierrez-Fernandez, J., Kaszuba, K., Minhas, G.S. et al. Nat Commun (2020) 11:4135-4135. DOI 10.1038/s41467-020-17957-0 · PubMed

Other PDB entries of the same protein (UniProt Q56222 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3I9V 3.1 Å, Crystal structure of the hydrophilic domain of respiratory complex I from Thermus…
3IAM 3.1 Å, Crystal structure of the hydrophilic domain of respiratory complex I from Thermus…
6Y11 3.11 Å, Respiratory complex I from Thermus thermophilus
3IAS 3.15 Å, Crystal structure of the hydrophilic domain of respiratory complex I from Thermus…
6I1P 3.21 Å, Respiratory complex I from Thermus thermophilus with bound NADH
2FUG 3.3 Å, Crystal structure of the hydrophilic domain of respiratory complex I from Thermus…
4HEA 3.3 Å, Crystal structure of the entire respiratory complex I from Thermus thermophilus
6Q8W 3.4 Å, Respiratory complex I from Thermus thermophilus with bound Aureothin.
6Q8X 3.51 Å, Respiratory complex I from Thermus thermophilus with bound Pyridaben.
6I0D 3.6 Å, Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone
6Q8O 3.6 Å, Respiratory complex I from Thermus thermophilus with bound Piericidin A
6ZIY 4.25 Å, Respiratory complex I from Thermus thermophilus, NADH dataset, major state

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