7APK: Human THO - UAP56 complex

Structure of the human THO - UAP56 complex. Determined by electron microscopy at 3.3 Å resolution. Released 16 Dec 2020.

Method: Electron microscopy
Resolution: 3.3 Å
Organism: Homo sapiens
Chains: 30
Atoms: 72,697
Mol. weight: 1836.84 kDa
Released: 16 Dec 2020

Explore 7APK in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7APK contains 386 α-helices and 328 β-strands across 30 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains a and A: 14 helices, 0 β-strands

Element	Residues	Length
α-helix	11-21	11
α-helix	30-36	7
α-helix	46-64	19
α-helix	72-83	12
α-helix	92-103	12
α-helix	107-119	13
α-helix	134-146	13
α-helix	153-166	14
α-helix	232-246	15
α-helix	255-274	20
α-helix	310-313	4
α-helix	315-333	19
α-helix	347-365	19
α-helix	371-391	21

Chains b and B: 46 helices, 0 β-strands

Element	Residues	Length
α-helix	169-179	11
α-helix	189-203	15
α-helix	207-220	14
α-helix	225-236	12
α-helix	242-254	13
α-helix	264-273	10
α-helix	282-287	6
α-helix	296-306	11
α-helix	344-352	9
α-helix	359-365	7
α-helix	377-390	14
α-helix	392-397	6
α-helix	425-438	14
α-helix	446-462	17
α-helix	477-496	20
α-helix	504-512	9
α-helix	518-525	8
α-helix	536-553	18
α-helix	564-574	11
α-helix	576-589	14
α-helix	594-600	7
α-helix	601-603	3
α-helix	607-619	13
α-helix	637-652	16
α-helix	658-669	12
α-helix	674-686	13
α-helix	697-700	4
α-helix	707-713	7
α-helix	724-732	9
α-helix	741-748	8
α-helix	754-756	3
α-helix	764-785	22
α-helix	857-866	10
α-helix	873-883	11
α-helix	890-892	3
α-helix	897-907	11
α-helix	928-958	31
α-helix	972-987	16
α-helix	992-1004	13
α-helix	1016-1020	5
α-helix	1025-1029	5
α-helix	1035-1053	19
α-helix	1088-1112	25
α-helix	1119-1130	12
α-helix	1138-1153	16
α-helix	1161-1174	14

Chains c, C, k and K: 2 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	29-41	13
β-strand	49-50	2	53
β-strand	58-64	7	54
β-strand	69-74	6	54
β-strand	79-84	6	54
β-strand	89-95	7	54
β-strand	102-107	6	55
β-strand	114-119	6	55
β-strand	123-128	6	55
β-strand	134-139	6	55
β-strand	146-149	4	56
β-strand	155-159	5	56
β-strand	164-169	6	56
β-strand	175-180	6	56
β-strand	188-190	3	57
β-strand	196-200	5	57
β-strand	206-210	5	57
β-strand	215-220	6	57
α-helix	226	1
β-strand	227-232	6	58
β-strand	238-243	6	58
β-strand	248-252	5	58
β-strand	258-262	5	58
β-strand	269-274	6	59
β-strand	280-285	6	59
β-strand	289-294	6	59
β-strand	300-305	6	59
β-strand	310-314	5	53
β-strand	321-326	6	53
β-strand	342-346	5	53

Chains e and E: 20 helices, 15 β-strands

Element	Residues	Length	Sheet
α-helix	42-46	5
α-helix	56-73	18
α-helix	82-136	55
α-helix	190-246	57
α-helix	253-261	9
α-helix	266-281	16
β-strand	286-291	6	60
α-helix	294-298	5
α-helix	334-340	7
β-strand	343	1	60
β-strand	347-354	8	60
β-strand	358-367	10	60
β-strand	372-381	10	60
α-helix	416-423	8
α-helix	431-437	7
β-strand	439	1	60
α-helix	442-448	7
α-helix	465-496	32
α-helix	507-510	4
β-strand	518-525	8	61
α-helix	527-531	5
α-helix	537-542	6
β-strand	550-558	9	61
β-strand	561-569	9	61
β-strand	579-585	7	61
β-strand	590	1	61
α-helix	596-602	7
α-helix	603-607	5
α-helix	609-611	3
α-helix	621-638	18
β-strand	663	1	62
β-strand	668	1	62
α-helix	669-670	2
β-strand	672-674	3	63
β-strand	679-681	3	63

Chains f, F, n and N: 4 helices, 32 β-strands

Element	Residues	Length	Sheet
α-helix	13-23	11
β-strand	30-32	3	64
β-strand	38-42	5	64
β-strand	47-52	6	64
α-helix	53-56	4
α-helix	63-65	3
β-strand	70-73	4	64
β-strand	81-84	4	65
β-strand	88-92	5	65
β-strand	97-101	5	65
α-helix	102-105	4
β-strand	112-116	5	65
β-strand	129-135	7	66
β-strand	140-145	6	66
β-strand	150-154	5	66
β-strand	160-164	5	66
β-strand	171-174	4	67
β-strand	184-187	4	67
β-strand	191-196	6	67
β-strand	201-207	7	67
β-strand	223-225	3	68
β-strand	228	1	69
β-strand	232-235	4	69
β-strand	236-237	2	68
β-strand	241-245	5	69
β-strand	250-255	6	69
β-strand	261-266	6	70
β-strand	270-275	6	70
β-strand	279-284	6	70
β-strand	289-294	6	70
β-strand	300-305	6	71
β-strand	317-321	5	71
β-strand	325-329	5	71
β-strand	331	1	72
β-strand	334	1	72
β-strand	337-340	4	71

Chains g and G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	22-38	17
α-helix	45-127	83
α-helix	134-180	47

Chains h, H, p and P: 5 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	262-268	7	73
α-helix	274-284	11
β-strand	289-293	5	73
α-helix	297-309	13
β-strand	314-317	4	73
α-helix	323-334	12
β-strand	340-343	4	73
β-strand	356-361	6	73
α-helix	368-375	8
β-strand	385-391	7	73
α-helix	394-407	14
β-strand	412-413	2	73

Chains i and I: 14 helices, 0 β-strands

Element	Residues	Length
α-helix	11-21	11
α-helix	30-36	7
α-helix	45-64	20
α-helix	71-83	13
α-helix	92-103	12
α-helix	107-118	12
α-helix	134-146	13
α-helix	153-166	14
α-helix	232-246	15
α-helix	248-250	3
α-helix	255-274	20
α-helix	316-332	17
α-helix	347-365	19
α-helix	371-391	21

4 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
THO complex subunit 1	A, I, a, i	protein	711	Homo sapiens	Q96FV9 (AlphaFold model)
THO complex subunit 2	B, J, b, j	protein	1226	Homo sapiens	Q8NI27 (AlphaFold model)
THO complex subunit 3	C, K, c, k	protein	395	Homo sapiens	Q96J01 (AlphaFold model)
THO complex subunit 5 homolog	E, M, e, m	protein	683	Homo sapiens	Q13769 (AlphaFold model)
THO complex subunit 6 homolog	F, N, f, n	protein	341	Homo sapiens	Q86W42
THO complex subunit 7 homolog	G, O, g, o	protein	204	Homo sapiens	Q6I9Y2
Spliceosome RNA helicase DDX39B	H, P, h, p	protein	451	Homo sapiens	Q13838
THOC2 anchor (putative)	X, x	protein	37	Homo sapiens

Sequence of entity 1 (A, I, a, i), FASTA

>7APK_1 THO complex subunit 1 (chains A, I, a, i)
MGKPIPNPLLGLDSTGSGKPIPNPLLGLDSTGSGKPIPNPLLGLDSTLEVLFQGPSPTPP
LFSLPEARTRFTKSTREALNNKNIKPLLSTFSQVPGSENEKKCTLDQAFRGILEEEIINH
SSCENVLAIISLAIGGVTEGICTASTPFVLLGDVLDCLPLDQCDTIFTFVEKNVATWKSN
TFYSAGKNYLLRMCNDLLRRLSKSQNTVFCGRIQLFLARLFPLSEKSGLNLQSQFNLENV
TVFNTNEQESTLGQKHTEDREEGMDVEEGEMGDEEAPTTCSIPIDYNLYRKFWSLQDYFR
NPVQCYEKISWKTFLKYSEEVLAVFKSYKLDDTQASRKKMEELKTGGEHVYFAKFLTSEK
LMDLQLSDSNFRRHILLQYLILFQYLKGQVKFKSSNYVLTDEQSLWIEDTTKSVYQLLSE
NPPDGERFSKMVEHILNTEENWNSWKNEGCPSFVKERTSDTKPTRIIRKRTAPEDFLGKG
PTKKILMGNEELTRLWNLCPDNMEACKSETREHMPTLEEFFEEAIEQADPENMVENEYKA
VNNSNYGWRALRLLARRSPHFFQPTNQQFKSLPEYLENMVIKLAKELPPPSEEIKTGEDE
DEEDNDALLKENESPDVRRDKPVTGEQIEVFANKLGEQWKILAPYLEMKDSEIRQIECDS
EDMKMRAKQLLVAWQDQEGVHATPENLINALNKSGLSDLAESLTNDNETNS

Sequence of entity 2 (B, J, b, j), FASTA

>7APK_2 THO complex subunit 2 (chains B, J, b, j)
MKHHHHHHHHHHSAGLEVLFQGPMAAAAVVVPAEWIKNWEKSGRGEFLHLCRILSENKSH
DSSTYRDFQQALYELSYHVIKGNLKHEQASNVLSDISEFREDMPSILADVFCILDIETNC
LEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFY
KQQKFNLLREENEGYAKLIAELGQDLSGSITSDLILENIKSLIGCFNLDPNRVLDVILEV
FECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFN
LIDLDDLYVHLLPADNCIMDEHKREIAEAKQIVRKLTMVVLSSEKMDEREKEKEKEEEKV
EKPPDNQKLGLLEALLKIGDWQHAQNIMDQMPPYYAASHKLIALAICKLIHITIEPLYRR
VGVPKGAKGSPVNALQNKRAPKQAESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGK
SFMKEFQSDGSKQEDKEKTEVILSCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPY
QHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPT
ILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKERMKHDDTTISS
WLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTM
EQLEAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQE
GGEKHLKLVGKLYDQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFL
SRPMYAHHISSKYDELKKSEKGSKQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDIS
PQFYATFWSLTMYDLAVPHTSYEREVNKLKVQMKAIDDNQEMPPNKKKKEKERCTALQDK
LLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCA
RFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDR
ATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEY
THIRNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRK
SYMIPENEFHHKDPPPRNAVASVQNG

Sequence of entity 3 (C, K, c, k), FASTA

>7APK_3 THO complex subunit 3 (chains C, K, c, k)
MKGSAWSHPQFEKGGGSGGGSGGSAWSHPQFEKTAGLEVLFQGPMAVPAAAMGPSALGQS
GPGSMAPWCSVSSGPSRYVLGMQELFRGHSKTREFLAHSAKVHSVAWSCDGRRLASGSFD
KTASVFLLEKDRLVKENNYRGHGDSVDQLCWHPSNPDLFVTASGDKTIRIWDVRTTKCIA
TVNTKGENINICWSPDGQTIAVGNKDDVVTFIDAKTHRSKAEEQFKFEVNEISWNNDNNM
FFLTNGNGCINILSYPELKPVQSINAHPSNCICIKFDPMGKYFATGSADALVSLWDVDEL
VCVRCFSRLDWPVRTLSFSHDGKMLASASEDHFIDIAEVETGDKLWEVQCESPTFTVAWH
PKRPLLAFACDDKDGKYDSSREAGTVKLFGLPNDS

Sequence of entity 4 (E, M, e, m), FASTA

>7APK_4 THO complex subunit 5 homolog (chains E, M, e, m)
MSSESSKKRKPKVIRSDGAPAEGKRNRSDTEQEGKYYSEEAEVDLRDPGRDYELYKYTCQ
ELQRLMAEIQDLKSRGGKDVAIEIEERRIQSCVHFMTLKKLNRLAHIRLKKGRDQTHEAK
QKVDAYHLQLQNLLYEVMHLQKEITKCLEFKSKHEEIDLVSLEEFYKEAPPDISKAEVTM
GDPHQQTLARLDWELEQRKRLAEKYRECLSNKEKILKEIEVKKEYLSSLQPRLNSIMQAS
LPVQEYLFMPFDQAHKQYETARHLPPPLYVLFVQATAYGQACDKTLSVAIEGSVDEAKAL
FKPPEDSQDDESDSDAEEEQTTKRRRPTLGVQLDDKRKEMLKRHPLSVMLDLKCKDDSVL
HLTFYYLMNLNIMTVKAKVTTAMELITPISAGDLLSPDSVLSCLYPGDHGKKTPNPANQY
QFDKVGILTLSDYVLELGHPYLWVQKLGGLHFPKEQPQQTVIADHSLSASHMETTMKLLK
TRVQSRLALHKQFASLEHGIVPVTSDCQYLFPAKVVSRLVKWVTIAHEDYMELHFTKDIV
DAGLAGDTNLYYMALIERGTAKLQAAVVLNPGYSSIPPIFQLCLNWKGEKTNSNDDNIRA
MEGEVNVCYKELCGPWPSHQLLTNQLQRLCVLLDVYLETESHDDSVEGPKEFPQEKMCLR
LFRGPSRMKPFKYNHPQGFFSHR

Sequence of entity 5 (F, N, f, n), FASTA

>7APK_5 THO complex subunit 6 homolog (chains F, N, f, n)
MERAVPLAVPLGQTEVFQALQRLHMTIFSQSVSPCGKFLAAGNNYGQIAIFSLSSALSSE
AKEESKKPVVTFQAHDGPVYSMVSTDRHLLSAGDGEVKAWLWAEMLKKGCKELWRRQPPY
RTSLEVPEINALLLVPKENSLILAGGDCQLHTMDLETGTFTRVLRGHTDYIHCLALRERS
PEVLSGGEDGAVRLWDLRTAKEVQTIEVYKHEECSRPHNGRWIGCLATDSDWMVCGGGPA
LTLWHLRSSTPTTIFPIRAPQKHVTFYQDLILSAGQGRCVNQWQLSGELKAQVPGSSPGL
LSLSLNQQPAAPECKVLTAAGNSCRVDVFTNLGYRAFSLSF

Sequence of entity 6 (G, O, g, o), FASTA

>7APK_6 THO complex subunit 7 homolog (chains G, O, g, o)
MGAVTDDEVIRKRLLIDGDGAGDDRRINLLVKSFIKWCNSGSQEEGYSQYQRMLSTLSQC
EFSMGKTLLVYDMNLREMENYEKIYKEIECSIAGAHEKIAECKKQILQAKRIRKNRQEYD
ALAKVIQHHPDRHETLKELEALGKELEHLSHIKESVEDKLELRRKQFHVLLSTIHELQQT
LENDEKLSEVEEAQEASMETDPKP

Sequence of entity 7 (H, P, h, p), FASTA

>7APK_7 Spliceosome RNA helicase DDX39B (chains H, P, h, p)
GPMKGSAWSHPQFEKLEVLFQGPMAENDVDNELLDYEDDEVETAAGGDGAEAPAKKDVKG
SYVSIHSSGFRDFLLKPELLRAIVDCGFEHPSEVQHECIPQAILGMDVLCQAKSGMGKTA
VFVLATLQQLEPVTGQVSVLVMCHTRELAFQISKEYERFSKYMPNVKVAVFFGGLSIKKD
EEVLKKNCPHIVVGTPGRILALARNKSLNLKHIKHFILDECDKMLEQLDMRRDVQEIFRM
TPHEKQVMMFSATLSKEIRPVCRKFMQDPMEIFVDDETKLTLHGLQQYYVKLKDNEKNRK
LFDLLDVLEFNQVVIFVKSVQRCIALAQLLVEQNFPAIAIHRGMPQEERLSRYQQFKDFQ
RRILVATNLFGRGMDIERVNIAFNYDMPEDSDTYLHRVARAGRFGTKGLAITFVSDENDA
KILNDVQDRFEVNISELPDEIDISSYIEQTR

Sequence of entity 8 (X, x), FASTA

>7APK_8 THOC2 anchor (putative) (chains X, x)
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Primary citation

Structure of the human core transcription-export complex reveals a hub for multivalent interactions. Puhringer, T., Hohmann, U., Fin, L. et al. Elife (2020) 9. DOI 10.7554/eLife.61503 · PubMed

Other PDB entries of the same protein (UniProt Q96FV9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7ZNL 3.45 Å, Structure of the human TREX core THO-UAP56 complex
7ZNK 3.9 Å, Structure of an endogenous human TREX complex bound to mRNA
8R7L 4.12 Å, Cryo-EM structure of the human TREX complex
1WXP Solution structure of the death domain of nuclear matrix protein p84

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