7B00: Human LAT2-4F2hc complex in the apo-state

Human LAT2-4F2hc complex in the apo-state. Determined by electron microscopy at 3.98 Å resolution. Released 27 Oct 2021.

Method
Electron microscopy
Resolution
3.98 Å
Organism
Homo sapiens
Chains
2
Atoms
7,389
Mol. weight
119.35 kDa
Ligands
AJP
Released
27 Oct 2021

Explore 7B00 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7B00 contains 51 α-helices and 19 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 27 helices, 2 β-strands

ElementResiduesLengthSheet
α-helix47-537
α-helix64-674
α-helix78-9922
α-helix107-1115
α-helix116-12510
α-helix140-1478
α-helix155-1573
α-helix158-1603
α-helix162-17716
α-helix182-1854
α-helix187-1893
α-helix192-21120
α-helix256-2594
α-helix273-28816
α-helix302-3087
α-helix320-3234
α-helix338-3447
β-strand35711
α-helix365-37612
α-helix377-3793
α-helix385-39511
α-helix398-41114
α-helix426-4338
α-helix435-4384
α-helix440-4434
α-helix452-4554
α-helix459-4624
α-helix477-49014
β-strand49411
Chain B: 24 helices, 17 β-strands
ElementResiduesLengthSheet
α-helix66-727
α-helix75-10531
α-helix107-1082
α-helix110-1123
α-helix117-1193
β-strand123-12532
α-helix129-1335
α-helix140-1456
α-helix147-1504
β-strand159-16022
α-helix161-1622
β-strand165-16623
β-strand174-17523
α-helix186-19611
β-strand204-20632
α-helix224-2274
α-helix229-23810
β-strand244-24742
α-helix249-2513
α-helix255-26915
β-strand275-27842
α-helix284-2907
β-strand298-30032
α-helix311-32515
α-helix346-3483
α-helix349-3568
β-strand364-36632
α-helix386-3883
α-helix404-4063
α-helix408-4114
α-helix419-4268
α-helix434-4374
β-strand439-44244
β-strand449-45574
β-strand461-46774
β-strand47315
β-strand491-49774
β-strand507-50934
β-strand51515
α-helix5161
β-strand519-52574

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Large neutral amino acids transporter small subunit 2Aprotein535Homo sapiensQ9UHI5 (AlphaFold model)
Isoform 2 of 4F2 cell-surface antigen heavy chainBprotein529Homo sapiensP08195 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>7B00_1 Large neutral amino acids transporter small subunit 2 (chains A)
MEEGARHRNNTEKKHPGGGESDASPEAGSGGGGVALKKEIGLVSACGIIVGNIIGSGIFV
SPKGVLENAGSVGLALIVWIVTGFITVVGALCYAELGVTIPKSGGDYSYVKDIFGGLAGF
LRLWIAVLVIYPTNQAVIALTFSNYVLQPLFPTCFPPESGLRLLAAICLLLLTWVNCSSV
RWATRVQDIFTAGKLLALALIIIMGIVQICKGEYFWLEPKNAFENFQEPDIGLVALAFLQ
GSFAYGGWNFLNYVTEELVDPYKNLPRAIFISIPLVTFVYVFANVAYVTAMSPQELLASN
AVAVTFGEKLLGVMAWIMPISVALSTFGGVNGSLFTSSRLFFAGAREGHLPSVLAMIHVK
RCTPIPALLFTCISTLLMLVTSDMYTLINYVGFINYLFYGVTVAGQIVLRWKKPDIPRPI
KINLLFPIIYLLFWAFLLVFSLWSEPVVCGIGLAIMLTGVPVYFLGVYWQHKPKCFSDFI
ELLTLVSQKMCVVVYPEVERGSGTEEANEDMEEQQQPMYQPTPTKDKDVAGQPQP
Sequence of entity 2 (B), FASTA
>7B00_2 Isoform 2 of 4F2 cell-surface antigen heavy chain (chains B)
MSQDTEVDMKEVELNELEPEKQPMNAASGAAMSLAGAEKNGLVKIKVAEDEAEAAAAAKF
TGLSKEELLKVAGSPGWVRTRWALLLLFWLGWLGMLAGAVVIIVRAPRCRELPAQKWWHT
GALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQID
PNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAG
VDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLT
SSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLP
GTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLS
LFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQAS
DLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA

Ligands and cofactors

IDNameFormulaCopies
AJPDigitoninC56 H92 O291

Primary citation

Structural basis for substrate specificity of heteromeric transporters of neutral amino acids. Rodriguez, C.F., Escudero-Bravo, P., Diaz, L. et al. Proc Natl Acad Sci U S A (2021) 118. DOI 10.1073/pnas.2113573118 · PubMed

Other PDB entries of the same protein (UniProt Q9UHI5 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

About this viewer

MolViewer shows 7B00 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.