7BPH: GppNHp-bound GNAS

Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13. Determined by X-ray diffraction at 1.57 Å resolution. Released 31 Mar 2021.

Method: X-ray diffraction
Resolution: 1.57 Å
Organisms: Homo sapiens, synthetic construct
Chains: 2
Atoms: 3,255
Mol. weight: 46.4 kDa
Ligands: MG, GNP
Released: 31 Mar 2021

Explore 7BPH in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7BPH contains 23 α-helices and 8 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 22 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	18-39	22
β-strand	41-47	7	1
α-helix	53-64	12
α-helix	89-91	3
α-helix	92-112	21
α-helix	122-124	3
α-helix	125-133	9
α-helix	144-154	11
α-helix	157-163	7
α-helix	166-168	3
α-helix	175-179	5
α-helix	182-185	4
α-helix	194-199	6
β-strand	207-214	8	1
β-strand	217-224	8	1
α-helix	228-237	10
β-strand	243-249	7	1
α-helix	250-254	5
β-strand	256	1	2
β-strand	264	1	2
α-helix	265-277	13
α-helix	280-282	3
β-strand	287-292	6	1
α-helix	294-303	10
α-helix	308-310	3
α-helix	313-317	5
α-helix	326-327	2
α-helix	332-349	18
β-strand	359-363	5	1
α-helix	369-393	25

Chain B: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	8	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	377	Homo sapiens	P63092 (AlphaFold model)
GN13	B	protein	16	synthetic construct

Sequence of entity 1 (A), FASTA

>7BPH_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
AHMSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRIL
HVNGFNGDSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNV
PDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDL
LRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVI
REDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFAR
YTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFN
DCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>7BPH_2 GN13 (chains B)
XYFESVYAIWGTLCGX

Ligands and cofactors

ID	Name	Formula	Copies
MG	Magnesium ion	Mg	1
GNP	Phosphoaminophosphonic acid-guanylate ester	C10 H17 N6 O13 P3	1

Water and common crystallization additives (CL, GOL) are not listed.

Primary citation

State-selective modulation of heterotrimeric G alpha s signaling with macrocyclic peptides. Dai, S.A., Hu, Q., Gao, R. et al. Cell (2022). DOI 10.1016/j.cell.2022.09.019 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)
7TYF 2.2 Å, Human Amylin1 Receptor in complex with Gs and rat amylin peptide

Browse structure collections

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