7F0U: Papain-like protease 2

porcine epidemic diarrhea virus papain-like protease 2 C44S mutant in complex with mono ubiquitin. Determined by X-ray diffraction at 2.2 Å resolution. Released 20 Oct 2021.

Method: X-ray diffraction
Resolution: 2.2 Å
Organisms: Porcine epidemic diarrhea virus, Bos taurus
Chains: 4
Atoms: 4,727
Mol. weight: 68.97 kDa
Ligands: ZN
Released: 20 Oct 2021

Explore 7F0U in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7F0U contains 29 α-helices and 52 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 18 β-strands

Element	Residues	Length	Sheet
α-helix	16-18	3
α-helix	22-24	3
β-strand	28-31	4	1
β-strand	34-37	4	1
α-helix	44-56	13
β-strand	60	1	2
α-helix	63-73	11
α-helix	78-88	11
α-helix	90-91	2
α-helix	98-105	8
α-helix	106-108	3
β-strand	109	1	2
α-helix	110-111	2
β-strand	113-120	8	3
β-strand	127-132	6	3
β-strand	136-138	3	4
β-strand	142	1	5
β-strand	146-150	5	3
β-strand	156-166	11	3
β-strand	167-171	5	4
β-strand	177	1	4
α-helix	181-183	3
β-strand	184	1	5
α-helix	185-186	2
β-strand	189-194	6	4
β-strand	203-208	6	4
β-strand	213-216	4	4
β-strand	219-222	4	4
α-helix	225-227	3
β-strand	230-236	7	4

Chain B: 9 helices, 19 β-strands

Element	Residues	Length	Sheet
α-helix	16-18	3
α-helix	22-24	3
β-strand	28-31	4	6
β-strand	34-37	4	6
α-helix	44-55	12
β-strand	60	1	7
α-helix	63-74	12
α-helix	78-88	11
β-strand	96	1	8
α-helix	98-105	8
α-helix	106-108	3
β-strand	109	1	7
β-strand	113-121	9	9
β-strand	129-132	4	9
β-strand	136-138	3	10
β-strand	142	1	11
β-strand	146-150	5	9
β-strand	156-166	11	9
β-strand	167-171	5	10
β-strand	177	1	10
α-helix	181-183	3
β-strand	184	1	11
β-strand	189-194	6	10
β-strand	203-208	6	10
β-strand	213-216	4	10
β-strand	219-222	4	10
α-helix	225-227	3
β-strand	230-236	7	10

Chain C: 4 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	2-6	5	12
β-strand	12-16	5	12
β-strand	22	1	13
α-helix	23-34	12
α-helix	38-40	3
β-strand	41-45	5	12
β-strand	48-49	2	12
β-strand	55	1	13
α-helix	57-59	3
β-strand	66-71	6	12
α-helix	72-73	2

Chain D: 4 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	2-6	5	14
β-strand	12-16	5	14
β-strand	22	1	15
α-helix	23-34	12
α-helix	38-40	3
β-strand	41-45	5	14
β-strand	48-49	2	14
α-helix	50-51	2
β-strand	55	1	15
β-strand	66-71	6	14
α-helix	72-73	2
β-strand	75	1	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
papain-like protease 2	A, B	protein	237	Porcine epidemic diarrhea virus	A0A075ECV2
Ubiquitin	C, D	protein	76	Bos taurus	P62992 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>7F0U_1 papain-like protease 2 (chains A, B)
SANWDSHYGFDKAGEFHMLDHTGFAFPSEVVNGRRVLKTTDNNSWVNVTCLQLQFARFRF
KSAGLQAMWESYCTGDVAMFVHWLYWLTGVDKGQPSDSENALNMLSKYIVPAGSVTIERV
THDGCCCSKRVVTAPVVNASVLKLGVEDGLCPHGLNYIDKVVVVKGTTIVVNVGKPVVAP
SHLFLKGVSYTTFLDNGNGVAGHYTVFDHDTGMVHDGDVFVPGDLNVSPVTNVVVSE

Sequence of entity 2 (C, D), FASTA

>7F0U_2 Ubiquitin (chains C, D)
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
IQKESTLHLVLRLRGG

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	2

Primary citation

Structural and Biochemical Characterization of Porcine Epidemic Diarrhea Virus Papain-Like Protease 2. Chu, H.F., Cheng, S.C., Sun, C.Y. et al. J Virol (2022) 96:e0137221-e0137221. DOI 10.1128/JVI.01372-21 · PubMed

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