7L2S: DkTx-bound minimal TRPV1 at the pre-bound state

cryo-EM structure of DkTx-bound minimal TRPV1 at the pre-bound state. Determined by electron microscopy at 2.71 Å resolution. Released 22 Sept 2021.

Method

Electron microscopy

Resolution

2.71 Å

Organism

Rattus norvegicus

Chains

4

Atoms

20,579

Mol. weight

296.75 kDa

Ligands

6IY, XJ7

Released

22 Sept 2021

Explore 7L2S in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7L2S contains 150 α-helices and 38 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 39 helices, 9 β-strands

Molecules and chains

>7L2S_1 Transient receptor potential cation channel subfamily V member 1 (chains A, B, C, D)
GAMGSRLYDRRSIFDAVAQSNCQELESLLPFLQRSKKRLTDSEFKDPETGKTCLLKAMLN
LHNGQNDTIALLLDVARKTDSLKQFVNASYTDSYYKGQTALHIAIERRNMTLVTLLVENG
ADVQAAANGDFFKKTKGRPGFYFGELPLSLAACTNQLAIVKFLLQNSWQPADISARDSVG
NTVLHALVEVADNTVDNTKFVTSMYNEILILGAKLHPTLKLEEITNRKGLTPLALAASSG
KIGVLAYILQREIHEPECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSS
ETPNRHDMLLVEPLNRLLQDKWDRFVKRIFYFNFFVYCLYMIIFTAAAYYRPVEGLPPYK
LKNTVGDYFRVTGEILSVSGGVYFFFRGIQYFLQRRPSLKSLFVDSYSEILFFVQSLFML
VSVVLYFSQRKEYVASMVFSLAMGWTNMLYYTRGFQQMGIYAVMIEKMILRDLCRFMFVY
LVFLFGFSTAVVTLIEDGKYNSLYSTCLELFKFTIGMGDLEFTENYDFKAVFIILLLAYV
ILTYILLLNMLIALMGETVNKIAQESKNIWKLQRAITILDTEKSFLKCMRKAFRSGKLLQ
VGFTPDGKDDYRWCFRVDEVNWTTWNTNVGIINEDPG

Ligands and cofactors

Water and common crystallization additives (NA) are not listed.

Primary citation

Structural snapshots of TRPV1 reveal mechanism of polymodal functionality. Zhang, K., Julius, D., Cheng, Y. Cell (2021) 184:5138-5150.e12. DOI 10.1016/j.cell.2021.08.012 · PubMed

Related structures

Other PDB entries of the same protein (UniProt O35433 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 3SUI 1.95 Å, Crystal structure of ca2+-calmodulin in complex with a trpv1 c-terminal peptide
• 8U4D 2.2 Å, TRPV1 in nanodisc bound with PI-Br4, consensus structure
• 8U3A 2.3 Å, TRPV1 in nanodisc bound with PI-Br4 bound in Conformation 1 (monomer)
• 8U3C 2.3 Å, TRPV1 in nanodisc bound with PI-Br4 bound in Conformation 2 (monomer)
• 8U43 2.4 Å, TRPV1 in nanodisc bound with PIP2-Br4
• 7L2P 2.6 Å, cryo-EM structure of unliganded minimal TRPV1
• 7L2H 2.63 Å, Cryo-EM structure of unliganded full-length TRPV1 at neutral pH
• 7LP9 2.63 Å, Cryo-EM structure of full-length TRPV1 at 4 degrees Celsius
• 2PNN 2.7 Å, Crystal Structure of the Ankyrin Repeat Domain of Trpv1
• 9W4M 2.7 Å, ratTRPV1 bound with antagonist AMG517
• 7MZ5 2.76 Å, Cryo-EM structure of RTX-bound full-length TRPV1 in C2 state
• 9W4T 2.87 Å, ratTRPV1 bound with antagonist AMG9810

Browse structure collections

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