7MF3: Autoinhibited state of smooth muscle myosin-2
Structure of the autoinhibited state of smooth muscle myosin-2. Determined by electron microscopy at 3.4 Å resolution. Released 5 Jan 2022.
- Method
- Electron microscopy
- Resolution
- 3.4 Å
- Organism
- Gallus gallus
- Chains
- 8
- Atoms
- 22,938
- Mol. weight
- 990.45 kDa
- Ligands
- MG, PO4, ADP
- Released
- 5 Jan 2022
Explore 7MF3 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7MF3 contains 120 α-helices and 57 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 44 helices, 30 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 35-39 | 5 | 1 |
| β-strand | 43-49 | 7 | 1 |
| β-strand | 53 | 1 | 1 |
| β-strand | 56-61 | 6 | 1 |
| β-strand | 67-71 | 5 | 1 |
| β-strand | 76-77 | 2 | 1 |
| β-strand | 88 | 1 | 2 |
| α-helix | 89-91 | 3 | |
| α-helix | 97-109 | 13 | |
| β-strand | 115-117 | 3 | 2 |
| β-strand | 120-124 | 5 | 2 |
| α-helix | 135-138 | 4 | |
| α-helix | 139-141 | 3 | |
| α-helix | 150-151 | 2 | |
| α-helix | 153-167 | 15 | |
| β-strand | 171-172 | 2 | 3 |
| β-strand | 174-177 | 4 | 2 |
| β-strand | 178 | 1 | 4 |
| α-helix | 183-197 | 15 | |
| α-helix | 219-234 | 16 | |
| β-strand | 238 | 1 | 5 |
| β-strand | 249-255 | 7 | 3 |
| β-strand | 264-270 | 7 | 3 |
| β-strand | 285 | 1 | 5 |
| α-helix | 288-294 | 7 | |
| α-helix | 299-303 | 5 | |
| α-helix | 310-312 | 3 | |
| α-helix | 328-342 | 15 | |
| α-helix | 346-361 | 16 | |
| α-helix | 362-364 | 3 | |
| β-strand | 368-369 | 2 | 6 |
| β-strand | 376-377 | 2 | 6 |
| α-helix | 382-391 | 10 | |
| α-helix | 395-403 | 9 | |
| α-helix | 405 | 1 | |
| β-strand | 406-407 | 2 | 7 |
| β-strand | 414-415 | 2 | 7 |
| α-helix | 420-448 | 29 | |
| β-strand | 461-462 | 2 | 3 |
| β-strand | 465 | 1 | 3 |
| α-helix | 467-468 | 2 | |
| β-strand | 469 | 1 | 4 |
| α-helix | 477-494 | 18 | |
| α-helix | 495-499 | 5 | |
| α-helix | 500-507 | 8 | |
| α-helix | 522-528 | 7 | |
| α-helix | 538-545 | 8 | |
| α-helix | 553-563 | 11 | |
| β-strand | 583-584 | 2 | 8 |
| β-strand | 589-590 | 2 | 8 |
| α-helix | 597-602 | 6 | |
| α-helix | 607-614 | 8 | |
| α-helix | 619-626 | 8 | |
| α-helix | 659-676 | 18 | |
| β-strand | 678 | 1 | 3 |
| α-helix | 679 | 1 | |
| β-strand | 681-685 | 5 | 2 |
| α-helix | 698-706 | 9 | |
| α-helix | 710-718 | 9 | |
| β-strand | 723-726 | 4 | 9 |
| α-helix | 727-730 | 4 | |
| α-helix | 731-735 | 5 | |
| α-helix | 749-760 | 12 | |
| α-helix | 764-766 | 3 | |
| β-strand | 767-768 | 2 | 9 |
| β-strand | 773-776 | 4 | 9 |
| α-helix | 778-783 | 6 | |
| α-helix | 785-791 | 7 | |
| α-helix | 793-819 | 27 | |
| α-helix | 820-824 | 5 | |
| α-helix | 825-839 | 15 | |
| α-helix | 841-845 | 5 | |
| α-helix | 848-853 | 6 | |
| α-helix | 855-948 | 94 | |
Chain B: 33 helices, 23 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 35-38 | 4 | 10 |
| β-strand | 44-47 | 4 | 10 |
| β-strand | 53 | 1 | 11 |
| β-strand | 56 | 1 | 11 |
| β-strand | 57-60 | 4 | 12 |
| β-strand | 67-70 | 4 | 12 |
| β-strand | 76 | 1 | 10 |
| β-strand | 88 | 1 | 13 |
| α-helix | 98-109 | 12 | |
| β-strand | 116-117 | 2 | 13 |
| β-strand | 120-124 | 5 | 13 |
| α-helix | 135-140 | 6 | |
| α-helix | 153-166 | 14 | |
| β-strand | 171-176 | 6 | 13 |
| α-helix | 185-197 | 13 | |
| α-helix | 219-233 | 15 | |
| β-strand | 250-256 | 7 | 13 |
| β-strand | 267-268 | 2 | 13 |
| α-helix | 274-276 | 3 | |
| α-helix | 288-293 | 6 | |
| α-helix | 299-304 | 6 | |
| α-helix | 329-342 | 14 | |
| α-helix | 346-363 | 18 | |
| α-helix | 382-389 | 8 | |
| α-helix | 395-403 | 9 | |
| β-strand | 406 | 1 | 14 |
| β-strand | 415 | 1 | 14 |
| α-helix | 420-449 | 30 | |
| β-strand | 459-465 | 7 | 13 |
| α-helix | 477-495 | 19 | |
| α-helix | 496-500 | 5 | |
| α-helix | 501-508 | 8 | |
| α-helix | 522-526 | 5 | |
| α-helix | 537-546 | 10 | |
| α-helix | 552-563 | 12 | |
| β-strand | 579-585 | 7 | 15 |
| β-strand | 588-593 | 6 | 15 |
| α-helix | 597-601 | 5 | |
| α-helix | 608-614 | 7 | |
| α-helix | 621-626 | 6 | |
| α-helix | 630-631 | 2 | |
| α-helix | 662-674 | 13 | |
| β-strand | 678 | 1 | 13 |
| β-strand | 681-685 | 5 | 13 |
| α-helix | 698-708 | 11 | |
| α-helix | 710-719 | 10 | |
| β-strand | 724-726 | 3 | 16 |
| α-helix | 727-733 | 7 | |
| α-helix | 749-760 | 12 | |
| α-helix | 764-766 | 3 | |
| β-strand | 768-769 | 2 | 16 |
| β-strand | 773-775 | 3 | 16 |
| α-helix | 780-838 | 59 | |
| α-helix | 840-844 | 5 | |
| α-helix | 848-852 | 5 | |
| α-helix | 856-947 | 92 | |
Chain C: 8 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-17 | 13 | |
| α-helix | 28-37 | 10 | |
| α-helix | 46-51 | 6 | |
| α-helix | 66-77 | 12 | |
| α-helix | 85-95 | 11 | |
| α-helix | 105-113 | 9 | |
| α-helix | 122-126 | 5 | |
| α-helix | 141-147 | 7 | |
Chain D: 11 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 28-40 | 13 | |
| α-helix | 50-60 | 11 | |
| α-helix | 69-75 | 7 | |
| α-helix | 80-81 | 2 | |
| α-helix | 82-90 | 9 | |
| α-helix | 99-109 | 11 | |
| α-helix | 119-123 | 5 | |
| α-helix | 124-128 | 5 | |
| α-helix | 135-138 | 4 | |
| α-helix | 140-143 | 4 | |
| α-helix | 156-162 | 7 | |
Chain E: 12 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 27-40 | 14 | |
| α-helix | 50-60 | 11 | |
| α-helix | 68-73 | 6 | |
| α-helix | 84-92 | 9 | |
| α-helix | 99-109 | 11 | |
| β-strand | 117-118 | 2 | 17 |
| α-helix | 119-122 | 4 | |
| α-helix | 123-128 | 6 | |
| α-helix | 136-144 | 9 | |
| α-helix | 146-148 | 3 | |
| α-helix | 149-151 | 3 | |
| β-strand | 152-153 | 2 | 17 |
| α-helix | 155-160 | 6 | |
| α-helix | 161-165 | 5 | |
Chain F: 8 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-17 | 13 | |
| β-strand | 26 | 1 | 18 |
| α-helix | 28-37 | 10 | |
| α-helix | 46-49 | 4 | |
| β-strand | 64 | 1 | 18 |
| α-helix | 66-76 | 11 | |
| α-helix | 85-95 | 11 | |
| α-helix | 105-114 | 10 | |
| α-helix | 121-127 | 7 | |
| α-helix | 142-147 | 6 | |
Chain G: 2 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 1414-1526 | 113 | |
| α-helix | 1533-1622 | 90 | |
Chain H: 2 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 1415-1523 | 109 | |
| α-helix | 1536-1620 | 85 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Myosin-11 | A, B, G, H | protein | 1978 | Gallus gallus | P10587 (AlphaFold model) |
| Myosin light polypeptide 6 | C, F | protein | 150 | Gallus gallus | P02607 (AlphaFold model) |
| Myosin regulatory light chain 2, smooth muscle major isoform | D, E | protein | 171 | Gallus gallus | P02612 (AlphaFold model) |
Sequence of entity 1 (A, B, G, H), FASTA
>7MF3_1 Myosin-11 (chains A, B, G, H)
SQKPLSDDEKFLFVDKNFVNNPLAQADWSAKKLVWVPSEKHGFEAASIKEEKGDEVTVEL
QENGKKVTLSKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFC
VVINPYKQLPIYSEKIIDMYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGA
GKTENTKKVIQYLAVVASSHKGKKDTSITQGPSFSYGELEKQLLQANPILEAFGNAKTVK
NDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRQAKDERTFHIFYYLIAGASEQM
RNDLLLEGFNNYTFLSNGHVPIPAQQDDEMFQETLEAMTIMGFTEEEQTSILRVVSSVLQ
LGNIVFKKERNTDQASMPDNTAAQKVCHLMGINVTDFTRSILTPRIKVGRDVVQKAQTKE
QADFAIEALAKAKFERLFRWILTRVNKALDKTKRQGASFLGILDIAGFEIFEINSFEQLC
INYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPTNPPGVLALL
DEECWFPKATDTSFVEKLIQEQGNHAKFQKSKQLKDKTEFCILHYAGKVTYNASAWLTKN
MDPLNDNVTSLLNQSSDKFVADLWKDVDRIVGLDQMAKMTESSLPSASKTKKGMFRTVGQ
LYKEQLTKLMTTLRNTNPNFVRCIIPNHEKRAGKLDAHLVLEQLRCNGVLEGIRICRQGF
PNRIVFQEFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFFRTGVL
AHLEEERDLKITDVIIAFQAQCRGYLARKAFAKRQQQLTAMKVIQRNCAAYLKLRNWQWW
RLFTKVKPLLQVTRQEEEMQAKDEELQRTKERQQKAEAELKELEQKHTQLCEEKNLLQEK
LQAETELYAEAEEMRVRLAAKKQELEEILHEMEARIEEEEERSQQLQAEKKKMQQQMLDL
EEQLEEEEAARQKLQLEKVTADGKIKKMEDDILIMEDQNNKLTKERKLLEERVSDLTTNL
AEEEEKAKNLTKLKNKHESMISELEVRLKKEEKSRQELEKIKRKLEGESSDLHEQIAELQ
AQIAELKAQLAKKEEELQAALARLEDETSQKNNALKKIRELESHISDLQEDLESEKAARN
KAEKQKRDLSEELEALKTELEDTLDTTATQQELRAKREQEVTVLKRALEEETRTHEAQVQ
EMRQKHTQAVEELTEQLEQFKRAKANLDKTKQTLEKDNADLANEIRSLSQAKQDVEHKKK
KLEVQLQDLQSKYSDGERVRTELNEKVHKLQIEVENVTSLLNEAESKNIKLTKDVATLGS
QLQDTQELLQEETRQKLNVTTKLRQLEDDKNSLQEQLDEEVEAKQNLERHISTLTIQLSD
SKKKLQEFTATVETMEEGKKKLQREIESLTQQFEEKAASYDKLEKTKNRLQQELDDLVVD
LDNQRQLVSNLEKKQKKFDQMLAEEKNISSKYADERDRAEAEAREKETKALSLARALEEA
LEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRTLEQQVEEMKTQLEELEDEL
QAAEDAKLRLEVNMQAMKSQFERDLQARDEQNEEKRRQLLKQLHEHETELEDERKQRALA
AAAKKKLEVDVKDLESQVDSANKAREEAIKQLRKLQAQMKDYQRDLDDARAAREEIFATA
RENEKKAKNLEAELIQLQEDLAAAERARKQADLEKEEMAEELASANSGRTSLQDEKRRLE
ARIAQLEEELDEEHSNIETMSDRMRKAVQQAEQLNNELATERATAQKNENARQQLERQNK
ELRSKLQEMEGAVKSKFKSTIAALEAKIASLEEQLEQEAREKQAAAKTLRQKDKKLKDAL
LQVEDERKQAEQYKDQAEKGNLRLKQLKRQLEEAEEESQRINANRRKLQRELDEATESND
ALGREVAALKSKLRRGNEPVSFAPPRRSGGRRVIENATDGGEEEIDGRDGDFNGKASE
Sequence of entity 2 (C, F), FASTA
>7MF3_2 Myosin light polypeptide 6 (chains C, F)
CDFSEEQTAEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAEVMKVLGNPKSDEMN
LKTLKFEQFLPMMQTIAKNKDQGCFEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMT
EEEVEQLVAGHEDSNGCINYEELVRMVLSG
Sequence of entity 3 (D, E), FASTA
>7MF3_3 Myosin regulatory light chain 2, smooth muscle major isoform (chains D, E)
SSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASM
GKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHED
HLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| MG | Magnesium ion | Mg | 4 |
| PO4 | Phosphate ion | O4 P | 2 |
| ADP | Adenosine-5'-diphosphate | C10 H15 N5 O10 P2 | 2 |
Primary citation
Cryo-EM structure of the autoinhibited state of myosin-2. Heissler, S.M., Arora, A.S., Billington, N. et al. Sci Adv (2021) 7:eabk3273-eabk3273. DOI 10.1126/sciadv.abk3273 · PubMed
Other PDB entries of the same protein (UniProt P10587 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 5M05 2.67 Å, Chicken smooth muscle myosin motor domain co-crystallized with the specific CK-571…
- 5T45 2.8 Å, Chicken smooth muscle myosin motor domain co-crystallized with the specific CK-571…
- 1BR2 2.9 Å, Smooth muscle myosin motor domain complexed with MGADP.ALF4
- 1BR1 3.5 Å, Smooth muscle myosin motor domain-essential light chain complex with MGADP.ALF4 bound at…
- 1BR4 3.62 Å, Smooth muscle myosin motor domain-essential light chain complex with MGADP.BEF3 bound at…
- 6BIH 6.0 Å, The Structure of the Actin-Smooth Muscle Myosin Motor Domain Complex in the Rigor State
- 8SYF 19.0 Å, Homology model of Acto-HMM complex in ADP-state. Chicken smooth muscle HMM and chicken…
- 1I84 20.0 Å, cryo-EM structure of the heavy meromyosin subfragment of chicken gizzard smooth muscle…
- 3DTP 20.0 Å, Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick…
- 3J04 20.0 Å, EM structure of the heavy meromyosin subfragment of Chick smooth muscle Myosin with…
Browse structure collections
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