7NHT: Akirin2 bound human proteasome
Akirin2 bound human proteasome. Determined by electron microscopy at 3.2 Å resolution. Released 1 Sept 2021.
- Method
- Electron microscopy
- Resolution
- 3.2 Å
- Organism
- Homo sapiens
- Chains
- 16
- Atoms
- 24,915
- Mol. weight
- 425.96 kDa
- Released
- 1 Sept 2021
Explore 7NHT in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7NHT contains 117 α-helices and 195 β-strands across 16 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 9 helices, 17 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-5 | 3 | |
| β-strand | 8 | 1 | 1 |
| β-strand | 12 | 1 | 2 |
| β-strand | 18 | 1 | 2 |
| α-helix | 19-29 | 11 | |
| β-strand | 34-39 | 6 | 3 |
| β-strand | 42-48 | 7 | 3 |
| β-strand | 56 | 1 | 4 |
| β-strand | 65-66 | 2 | 5 |
| β-strand | 71-77 | 7 | 5 |
| α-helix | 79-100 | 22 | |
| α-helix | 103-105 | 3 | |
| α-helix | 106-122 | 17 | |
| β-strand | 124 | 1 | 1 |
| β-strand | 126 | 1 | 6 |
| β-strand | 131-139 | 9 | 5 |
| β-strand | 142-148 | 7 | 5 |
| β-strand | 154-156 | 3 | 5 |
| β-strand | 157 | 1 | 7 |
| β-strand | 159-162 | 4 | 3 |
| α-helix | 166-175 | 10 | |
| α-helix | 183-197 | 15 | |
| β-strand | 207-213 | 7 | 3 |
| β-strand | 216-219 | 4 | 3 |
| α-helix | 220-221 | 2 | |
| α-helix | 222-230 | 9 | |
Chain B: 11 helices, 14 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 19-29 | 11 | |
| β-strand | 34-38 | 5 | 8 |
| β-strand | 43-48 | 6 | 8 |
| β-strand | 56 | 1 | 7 |
| β-strand | 66-69 | 4 | 9 |
| β-strand | 72-78 | 7 | 9 |
| β-strand | 79 | 1 | 10 |
| α-helix | 80-101 | 22 | |
| α-helix | 104-106 | 3 | |
| α-helix | 107-121 | 15 | |
| β-strand | 124 | 1 | 11 |
| α-helix | 127-129 | 3 | |
| β-strand | 132-140 | 9 | 9 |
| β-strand | 144-150 | 7 | 9 |
| β-strand | 156-158 | 3 | 9 |
| β-strand | 159 | 1 | 12 |
| β-strand | 161-164 | 4 | 8 |
| α-helix | 168-178 | 11 | |
| α-helix | 179-180 | 2 | |
| α-helix | 186-200 | 15 | |
| α-helix | 208-210 | 3 | |
| β-strand | 211-219 | 9 | 8 |
| β-strand | 222-227 | 6 | 8 |
| α-helix | 228-229 | 2 | |
| α-helix | 230-248 | 19 | |
Chain c: 1 helix, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 157-189 | 33 | |
| β-strand | 202 | 1 | 10 |
Chain C: 12 helices, 17 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 10 | 1 | 13 |
| α-helix | 11 | 1 | |
| α-helix | 12-14 | 3 | |
| β-strand | 16 | 1 | 13 |
| α-helix | 17-27 | 11 | |
| α-helix | 30-31 | 2 | |
| β-strand | 32-36 | 5 | 14 |
| β-strand | 40-46 | 7 | 14 |
| β-strand | 54 | 1 | 12 |
| α-helix | 56-59 | 4 | |
| β-strand | 62-64 | 3 | 15 |
| β-strand | 69-75 | 7 | 15 |
| β-strand | 76 | 1 | 16 |
| α-helix | 77-98 | 22 | |
| α-helix | 101-103 | 3 | |
| α-helix | 104-120 | 17 | |
| β-strand | 121 | 1 | 17 |
| β-strand | 124 | 1 | 11 |
| α-helix | 125-127 | 3 | |
| β-strand | 129-136 | 8 | 15 |
| β-strand | 142-147 | 6 | 15 |
| β-strand | 153-155 | 3 | 15 |
| β-strand | 156 | 1 | 18 |
| β-strand | 158-161 | 4 | 14 |
| α-helix | 165-175 | 11 | |
| α-helix | 184-196 | 13 | |
| β-strand | 206-212 | 7 | 14 |
| β-strand | 218-219 | 2 | 14 |
| α-helix | 222-237 | 16 | |
Chain d: 2 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 161-195 | 35 | |
| α-helix | 198-199 | 2 | |
| β-strand | 202 | 1 | 16 |
Chain D: 10 helices, 16 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-8 | 3 | |
| β-strand | 15 | 1 | 19 |
| β-strand | 21 | 1 | 19 |
| α-helix | 22-31 | 10 | |
| α-helix | 35-36 | 2 | |
| β-strand | 37-42 | 6 | 20 |
| β-strand | 45-51 | 7 | 20 |
| β-strand | 59 | 1 | 18 |
| β-strand | 67-71 | 5 | 21 |
| β-strand | 74-80 | 7 | 21 |
| α-helix | 82-103 | 22 | |
| α-helix | 106-108 | 3 | |
| α-helix | 109-117 | 9 | |
| β-strand | 123 | 1 | 17 |
| β-strand | 133 | 1 | 17 |
| β-strand | 139-147 | 9 | 21 |
| β-strand | 150-156 | 7 | 21 |
| β-strand | 162-164 | 3 | 21 |
| β-strand | 165 | 1 | 22 |
| β-strand | 167-170 | 4 | 20 |
| α-helix | 174-184 | 11 | |
| α-helix | 191-205 | 15 | |
| α-helix | 209-210 | 2 | |
| β-strand | 215-220 | 6 | 20 |
| β-strand | 226-228 | 3 | 20 |
| α-helix | 235-238 | 4 | |
Chain E: 12 helices, 12 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 20-31 | 12 | |
| α-helix | 33-34 | 2 | |
| β-strand | 35-39 | 5 | 23 |
| β-strand | 43-48 | 6 | 23 |
| β-strand | 57 | 1 | 22 |
| α-helix | 58-61 | 4 | |
| β-strand | 63-67 | 5 | 24 |
| β-strand | 70-76 | 7 | 24 |
| α-helix | 78-99 | 22 | |
| α-helix | 102-104 | 3 | |
| α-helix | 105-117 | 13 | |
| α-helix | 118-120 | 3 | |
| β-strand | 122 | 1 | 25 |
| β-strand | 130-138 | 9 | 24 |
| β-strand | 141-147 | 7 | 24 |
| β-strand | 153-156 | 4 | 24 |
| β-strand | 159-161 | 3 | 23 |
| α-helix | 165-174 | 10 | |
| α-helix | 176-179 | 4 | |
| α-helix | 184-198 | 15 | |
| β-strand | 211-216 | 6 | 23 |
| β-strand | 219-224 | 6 | 23 |
| α-helix | 226-229 | 4 | |
| α-helix | 230-233 | 4 | |
Chain F: 10 helices, 14 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 21-30 | 10 | |
| β-strand | 36-41 | 6 | 26 |
| β-strand | 44-52 | 9 | 26 |
| β-strand | 58 | 1 | 24 |
| β-strand | 66-70 | 5 | 27 |
| β-strand | 73-79 | 7 | 27 |
| α-helix | 81-102 | 22 | |
| α-helix | 105-107 | 3 | |
| α-helix | 108-120 | 13 | |
| α-helix | 121-123 | 3 | |
| β-strand | 125 | 1 | 28 |
| β-strand | 128 | 1 | 25 |
| β-strand | 133-141 | 9 | 27 |
| β-strand | 145-151 | 7 | 27 |
| β-strand | 157-159 | 3 | 27 |
| β-strand | 160 | 1 | 29 |
| β-strand | 162-165 | 4 | 26 |
| α-helix | 169-177 | 9 | |
| α-helix | 186-200 | 15 | |
| β-strand | 209-217 | 9 | 26 |
| α-helix | 218-220 | 3 | |
| β-strand | 225-226 | 2 | 26 |
| α-helix | 227-228 | 2 | |
| α-helix | 229-243 | 15 | |
8 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Proteasome subunit alpha type-2 | A | protein | 234 | Homo sapiens | P25787 (AlphaFold model) |
| Proteasome subunit alpha type-4 | B | protein | 261 | Homo sapiens | P25789 (AlphaFold model) |
| Proteasome subunit alpha type-7 | C | protein | 248 | Homo sapiens | O14818 (AlphaFold model) |
| Proteasome subunit alpha type-5 | D | protein | 241 | Homo sapiens | P28066 (AlphaFold model) |
| Proteasome subunit alpha type-1 | E | protein | 263 | Homo sapiens | P25786 |
| Proteasome subunit alpha type-3 | F | protein | 255 | Homo sapiens | P25788 |
| Proteasome subunit alpha type-6 | G | protein | 246 | Homo sapiens | P60900 |
| Proteasome subunit beta type-7 | H | protein | 277 | Homo sapiens | Q99436 |
| Proteasome subunit beta type-3 | I | protein | 205 | Homo sapiens | P49720 |
| Proteasome subunit beta type-2 | J | protein | 201 | Homo sapiens | P49721 |
| Proteasome subunit beta type-5 | K | protein | 263 | Homo sapiens | P28074 |
| Proteasome subunit beta type-1 | L | protein | 241 | Homo sapiens | P20618 |
3 more molecules are not listed.
Sequence of entity 1 (A), FASTA
>7NHT_1 Proteasome subunit alpha type-2 (chains A)
MAERGYSFSLTTFSPSGKLVQIEYALAAVAGGAPSVGIKAANGVVLATEKKQKSILYDER
SVHKVEPITKHIGLVYSGMGPDYRVLVHRARKLAQQYYLVYQEPIPTAQLVQRVASVMQE
YTQSGGVRPFGVSLLICGWNEGRPYLFQSDPSGAYFAWKATAMGKNYVNGKTFLEKRYNE
DLELEDAIHTAILTLKESFEGQMTEDNIEVGICNEAGFRRLTPTEVKDYLAAIA
Sequence of entity 2 (B), FASTA
>7NHT_2 Proteasome subunit alpha type-4 (chains B)
MSRRYDSRTTIFSPEGRLYQVEYAMEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVF
FSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQA
YTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYK
EGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQKEVEQLIKKH
EEEEAKAEREKKEKEQKEKDK
Sequence of entity 3 (C), FASTA
>7NHT_3 Proteasome subunit alpha type-7 (chains C)
MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGRDIVVLGVEKKSVAKLQDERTVR
KICALDDNVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQ
SNGRRPFGISALIVGFDFDGTPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDEA
IETDDLTIKLVIKALLEVVQSGGKNIELAVMRRDQSLKILNPEEIEKYVAEIEKEKEENE
KKKQKKAS
Sequence of entity 4 (D), FASTA
>7NHT_4 Proteasome subunit alpha type-5 (chains D)
MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGIQTSEGVCLAVEKRITSPLME
PSSIEKIVEIDAHIGCAMSGLIADAKTLIDKARVETQNHWFTYNETMTVESVTQAVSNLA
LQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSGTFVQCDARAIGSASEGAQSS
LQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELATVQPGQNFHMFTKEELEEVIKD
I
Sequence of entity 5 (E), FASTA
>7NHT_5 Proteasome subunit alpha type-1 (chains E)
MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQ
KKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPT
QRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSARTYLERHMSEFM
ECNLNELVKHGLRALRETLPAEQDLTTKNVSIGIVGKDLEFTIYDDDDVSPFLEGLEERP
QRKAQPAQPADEPAEKADEPMEH
Sequence of entity 6 (F), FASTA
>7NHT_6 Proteasome subunit alpha type-3 (chains F)
MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYE
EGSNKRLFNVDRHVGMAVAGLLADARSLADIAREEASNFRSNFGYNIPLKHLADRVAMYV
HAYTLYSAVRPFGCSFMLGSYSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKL
QMKEMTCRDIVKEVAKIIYIVHDEVKDKAFELELSWVGELTNGRHEIVPKDIREEAEKYA
KESLKEEDESDDDNM
Sequence of entity 7 (G), FASTA
>7NHT_7 Proteasome subunit alpha type-6 (chains G)
MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVIVTQKKVPDKL
LDSSTVTHLFKITENIGCVMTGMTADSRSQVQRARYEAANWKYKYGYEIPVDMLCKRIAD
ISQVYTQNAEMRPLGCCMILIGIDEEQGPQVYKCDPAGYYCGFKATAAGVKQTESTSFLE
KKVKKKFDWTFEQTVETAITCLSTVLSIDFKPSEIEVGVVTVENPKFRILTEAEIDAHLV
ALAERD
Sequence of entity 8 (H), FASTA
>7NHT_8 Proteasome subunit beta type-7 (chains H)
MAAVSVYAPPVGGFSFDNCRRNAVLEADFAKRGYKLPKVRKTGTTIAGVVYKDGIVLGAD
TRATEGMVVADKNCSKIHFISPNIYCCGAGTAADTDMTTQLISSNLELHSLSTGRLPRVV
TANRMLKQMLFRYQGYIGAALVLGGVDVTGPHLYSIYPHGSTDKLPYVTMGSGSLAAMAV
FEDKFRPDMEEEEAKNLVSEAIAAGIFNDLGSGSNIDLCVISKNKLDFLRPYTVPNKKGT
RLGRYRCEKGTTAVLTEKITPLEIEVLEETVQTMDTS
Sequence of entity 9 (I), FASTA
>7NHT_9 Proteasome subunit beta type-3 (chains I)
MSIMSYNGGAVMAMKGKNCVAIAADRRFGIQAQMVTTDFQKIFPMGDRLYIGLAGLATDV
QTVAQRLKFRLNLYELKEGRQIKPYTLMSMVANLLYEKRFGPYYTEPVIAGLDPKTFKPF
ICSLDLIGCPMVTDDFVVSGTCAEQMYGMCESLWEPNMDPDHLFETISQAMLNAVDRDAV
SGMGVIVHIIEKDKITTRTLKARMD
Sequence of entity 10 (J), FASTA
>7NHT_10 Proteasome subunit beta type-2 (chains J)
MEYLIGIQGPDYVLVASDRVAASNIVQMKDDHDKMFKMSEKILLLCVGEAGDTVQFAEYI
QKNVQLYKMRNGYELSPTAAANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDY
LAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKRFILNLPTFSV
RIIDKNGIHDLDNISFPKQGS
Sequence of entity 11 (K), FASTA
>7NHT_11 Proteasome subunit beta type-5 (chains K)
MALASVLERPLPVNQRGFFGLGGRADLLDLGPGSLSDGLSLAAPGWGVPEEPGIEMLHGT
TTLAFKFRHGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLAR
QCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDKRGPGLYYVDSEGNRI
SGATFSVGSGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDAYSGGAVNLYHVRE
DGWIRVSSDNVADLHEKYSGSTP
Sequence of entity 12 (L), FASTA
>7NHT_12 Proteasome subunit beta type-1 (chains L)
MLSSTAMYSAPGRDLGMEPHRAAGPLQLRFSPYVFNGGTILAIAGEDFAIVASDTRLSEG
FSIHTRDSPKCYKLTDKTVIGCSGFHGDCLTLTKIIEARLKMYKHSNNKAMTTGAIAAML
STILYSRRFFPYYVYNIIGGLDEEGKGAVYSFDPVGSYQRDSFKAGGSASAMLQPLLDNQ
VGFKNMQNVEHVPLSLDRAMRLVKDVFISAAERDVYTGDALRICIVTKEGIREETVSLRK
D
Primary citation
AKIRIN2 controls the nuclear import of proteasomes in vertebrates. de Almeida, M., Hinterndorfer, M., Brunner, H. et al. Nature (2021) 599:491-496. DOI 10.1038/s41586-021-04035-8 · PubMed
Other PDB entries of the same protein (UniProt P25787 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 5LE5 1.8 Å, Native human 20S proteasome at 1.8 Angstrom
- 9TMN 1.85 Å, Human sperm 20S proteasome complex isolated from native source
- 5LEY 1.9 Å, Human 20S proteasome complex with Oprozomib at 1.9 Angstrom
- 5LF4 1.99 Å, Human 20S proteasome complex with Delanzomib at 2.0 Angstrom
- 5LF1 2.0 Å, Human 20S proteasome complex with Dihydroeponemycin at 2.0 Angstrom
- 5LF7 2.0 Å, Human 20S proteasome complex with Ixazomib at 2.0 Angstrom
- 8UD9 2.04 Å, Structure of human constitutive 20S proteasome complexed with the inhibitor TDI-8304
- 5LF6 2.07 Å, Human 20S proteasome complex with Z-LLY-ketoaldehyde at 2.1 Angstrom
- 5LF3 2.1 Å, Human 20S proteasome complex with Bortezomib at 2.1 Angstrom
- 8BZL 2.14 Å, Human 20S Proteasome in complex with peptide activator peptide BLM42
- 5LEZ 2.19 Å, Human 20S proteasome complex with Oprozomib in Mg-Acetate at 2.2 Angstrom
- 5LEX 2.2 Å, Native human 20S proteasome in Mg-Acetate at 2.2 Angstrom
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