7O72: PDB entry 7O72

Name: PDB 7O72: Yeast RNA polymerase II transcription pre-initiation complex with closed promoter DNA
Published: 2021-06-16
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: transcription

Yeast RNA polymerase II transcription pre-initiation complex with closed promoter DNA. Determined by electron microscopy at 3.4 Å resolution. Released 16 Jun 2021.

Method: Electron microscopy
Resolution: 3.4 Å
Organisms: Saccharomyces cerevisiae S288C, synthetic construct, Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Chains: 30
Atoms: 73,664
Mol. weight: 1376.62 kDa
Ligands: ADP, MG, BEF, ZN
Released: 16 Jun 2021

Explore 7O72 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7O72 contains 463 α-helices and 436 β-strands across 28 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 0: 43 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	2-4	3	1
β-strand	9-11	3	1
β-strand	17	1	2
α-helix	19-34	16
β-strand	37-41	5	3
α-helix	49-62	14
β-strand	69-73	5	3
α-helix	76-96	21
β-strand	104-107	4	3
α-helix	111-113	3
α-helix	126-137	12
α-helix	140-147	8
α-helix	157-164	8
α-helix	167-169	3
β-strand	174-176	3	3
α-helix	178-188	11
α-helix	192-197	6
α-helix	200-202	3
β-strand	205-209	5	3
α-helix	211-214	4
α-helix	216-223	8
β-strand	231-235	5	3
α-helix	240-247	8
β-strand	250-253	4	4
α-helix	254-277	24
α-helix	280-291	12
α-helix	295-298	4
α-helix	313-315	3
α-helix	320-321	2
α-helix	328-344	17
β-strand	352-354	3	4
α-helix	356-366	11
α-helix	371-375	5
α-helix	377-387	11
α-helix	397-409	13
β-strand	416-421	6	4
β-strand	423	1	5
β-strand	432	1	5
β-strand	434-439	6	4
α-helix	447-452	6
β-strand	456-460	5	3
α-helix	468-472	5
β-strand	477-482	6	3
β-strand	492-499	8	6
β-strand	505	1	6
α-helix	510-512	3
α-helix	516-532	17
β-strand	536-541	6	6
α-helix	544-557	14
α-helix	559-566	8
β-strand	568-571	4	6
α-helix	576-591	16
β-strand	596-601	6	6
α-helix	606-608	3
α-helix	614-616	3
β-strand	619-623	5	6
α-helix	633-645	13
α-helix	650-666	17
β-strand	677-682	6	6
α-helix	684-688	5
α-helix	690-692	3
α-helix	695-698	4
α-helix	703-705	3
β-strand	708-709	2	6
α-helix	710-723	14
α-helix	727-729	3
α-helix	730-733	4
β-strand	739	1	2
α-helix	741-751	11

Chain 1: 37 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	4-9	6	8
β-strand	12-19	8	8
α-helix	25	1
β-strand	26-31	6	8
β-strand	37-41	5	8
α-helix	42-44	3
β-strand	45-50	6	8
β-strand	59-64	6	8
α-helix	65	1
β-strand	86-90	5	8
α-helix	94-120	27
α-helix	171-176	6
α-helix	178-187	10
α-helix	189-195	7
α-helix	196-201	6
α-helix	206-211	6
α-helix	214-225	12
α-helix	228-230	3
α-helix	233-236	4
β-strand	246-252	7	9
α-helix	253-262	10
α-helix	264-273	10
α-helix	280-289	10
α-helix	291-296	6
α-helix	309-312	4
α-helix	315-329	15
α-helix	335-337	3
α-helix	339-343	5
α-helix	352-354	3
α-helix	356-358	3
β-strand	362	1	10
β-strand	368	1	10
α-helix	371-389	19
α-helix	414-419	6
α-helix	422-424	3
α-helix	426-428	3
β-strand	432	1	11
α-helix	433	1
β-strand	434	1	12
α-helix	442-444	3
α-helix	466-482	17
α-helix	487-489	3
α-helix	494-514	21
α-helix	537-539	3
α-helix	544-568	25
α-helix	573-575	3
α-helix	576-604	29
α-helix	609-615	7
α-helix	617-638	22

Chain 2: 23 helices, 17 β-strands

Element	Residues	Length	Sheet
α-helix	6-16	11
α-helix	19-25	7
α-helix	29-37	9
α-helix	41-52	12
β-strand	57-58	2	13
α-helix	59-65	7
β-strand	66	1	14
α-helix	68-70	3
α-helix	71-83	13
β-strand	87-92	6	13
β-strand	95-100	6	13
α-helix	102-112	11
α-helix	131-136	6
α-helix	137-151	15
α-helix	161-169	9
β-strand	175-176	2	15
β-strand	181-182	2	15
α-helix	186-193	8
α-helix	196-210	15
α-helix	218-230	13
β-strand	237	1	16
α-helix	244-254	11
β-strand	259-261	3	16
β-strand	269-271	3	16
α-helix	273-277	5
β-strand	339-341	3	17
β-strand	346-349	4	17
α-helix	354-360	7
β-strand	365-370	6	17
β-strand	374-377	4	17
α-helix	380-388	9
α-helix	393-402	10
β-strand	404	1	17
α-helix	406-422	17
α-helix	435-448	14
β-strand	452-460	9	18
α-helix	465-477	13
β-strand	481-485	5	18
β-strand	490-494	5	18
α-helix	498-506	9

Chain 3: 6 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	28-29	2	23
β-strand	30	1	24
β-strand	38-39	2	23
α-helix	40-47	8
β-strand	52-53	2	25
β-strand	56	1	26
β-strand	59	1	26
β-strand	62-63	2	25
α-helix	65-67	3
β-strand	69	1	24
α-helix	76-89	14
α-helix	96-99	4
α-helix	103-122	20
α-helix	126-138	13

Chain 4: 16 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	24-31	8	29
α-helix	34-43	10
α-helix	49-67	19
β-strand	73-79	7	29
β-strand	82-87	6	29
α-helix	89-90	2
α-helix	116-138	23
α-helix	149-166	18
β-strand	173-181	9	29
α-helix	185-186	2
α-helix	193-206	14
β-strand	210-215	6	29
α-helix	222-230	9
β-strand	235-238	4	29
α-helix	244-250	7
α-helix	256-258	3
β-strand	262	1	14
β-strand	275	1	30
β-strand	282	1	30
β-strand	285-289	5	31
β-strand	295-296	2	31
α-helix	299-300	2
α-helix	301-303	3
β-strand	304	1	32
α-helix	310	1
β-strand	311	1	32
α-helix	312-313	2
α-helix	314-321	8
α-helix	322-325	4

Chain 5: 2 helices, 3 β-strands

Element	Residues	Length	Sheet
β-strand	3-11	9	18
α-helix	14-26	13
β-strand	31-36	6	18
β-strand	39-42	4	18
α-helix	44-59	16

Chain 6: 22 helices, 23 β-strands

Element	Residues	Length	Sheet
α-helix	97-113	17
β-strand	118	1	12
β-strand	120	1	33
β-strand	124-131	8	34
α-helix	134-137	4
α-helix	145-163	19
β-strand	168-175	8	34
β-strand	178-186	9	34
α-helix	189-199	11
α-helix	203-204	2
α-helix	210-220	11
β-strand	229-235	7	34
α-helix	247-256	10
β-strand	260-265	6	34
α-helix	271-279	9
β-strand	288-290	3	34
α-helix	294-303	10
α-helix	307-308	2
β-strand	309	1	33
α-helix	310	1
β-strand	311	1	11
β-strand	319-323	5	31
α-helix	325	1
β-strand	326	1	35
α-helix	327	1
β-strand	334-335	2	36
β-strand	342-343	2	36
β-strand	347-348	2	35
α-helix	349	1
β-strand	355-356	2	35
β-strand	361-362	2	31
β-strand	369-370	2	31
α-helix	373-377	5
α-helix	380-382	3
α-helix	385-389	5
β-strand	390-391	2	37
α-helix	392-393	2
β-strand	402	1	38
α-helix	408	1
β-strand	409	1	38
α-helix	410-411	2
β-strand	427-428	2	37
β-strand	435-436	2	37
α-helix	438-446	9
α-helix	452-456	5

Chain 7: 36 helices, 34 β-strands

Element	Residues	Length	Sheet
α-helix	105-107	3
α-helix	110-112	3
β-strand	117-118	2	17
α-helix	122-124	3
β-strand	132-134	3	39
β-strand	140-144	5	39
α-helix	150-160	11
β-strand	161-165	5	39
β-strand	170-175	6	39
α-helix	177-185	9
α-helix	190-200	11
β-strand	201	1	39
α-helix	204-206	3
α-helix	207-216	10
β-strand	223-224	2	40
β-strand	226-228	3	41
β-strand	231-235	5	41
α-helix	239-241	3
α-helix	242-245	4
β-strand	315-317	3	41
α-helix	323-332	10
β-strand	337-338	2	40
β-strand	340-341	2	42
α-helix	350	1
β-strand	351	1	43
β-strand	356	1	44
α-helix	364-374	11
β-strand	379-380	2	42
β-strand	382-385	4	45
α-helix	392-403	12
β-strand	405	1	43
β-strand	407-410	4	45
α-helix	414-427	14
β-strand	428	1	44
α-helix	432-434	3
β-strand	435-437	3	45
β-strand	452-455	4	45
α-helix	457-461	5
α-helix	468-479	12
β-strand	482	1	46
β-strand	484-488	5	45
α-helix	490-492	3
α-helix	499-504	6
β-strand	507	1	46
β-strand	510-514	5	45
α-helix	522-526	5
α-helix	527-530	4
β-strand	534-537	4	45
α-helix	541-545	5
β-strand	551-554	4	47
β-strand	555-559	5	48
α-helix	560-562	3
α-helix	563-571	9
α-helix	574-582	9
α-helix	585-600	16
β-strand	605-608	4	47
α-helix	612-622	11
α-helix	633-645	13
β-strand	651-654	4	47
α-helix	656-659	4
β-strand	669-672	4	47
α-helix	680-691	12
β-strand	702-707	6	47
β-strand	708-710	3	48
α-helix	715-722	8
α-helix	724-729	6
α-helix	733	1
β-strand	734-736	3	48
α-helix	753-764	12
α-helix	832-835	4

20 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
General transcription and DNA repair factor IIH helicase subunit XPD	0	protein	778	Saccharomyces cerevisiae S288C	P06839 (AlphaFold model)
General transcription and DNA repair factor IIH subunit TFB1	1	protein	645	Saccharomyces cerevisiae S288C	P32776 (AlphaFold model)
General transcription and DNA repair factor IIH subunit TFB2	2	protein	517	Saccharomyces cerevisiae S288C	Q02939 (AlphaFold model)
RNA polymerase II transcription factor B subunit 3	3	protein	324	Saccharomyces cerevisiae S288C	Q03290 (AlphaFold model)
General transcription and DNA repair factor IIH subunit TFB4	4	protein	341	Saccharomyces cerevisiae S288C	Q12004
General transcription and DNA repair factor IIH subunit TFB5	5	protein	76	Saccharomyces cerevisiae S288C	Q3E7C1
General transcription and DNA repair factor IIH subunit SSL1	6	protein	464	Saccharomyces cerevisiae S288C	Q04673
General transcription and DNA repair factor IIH helicase subunit XPB	7	protein	843	Saccharomyces cerevisiae S288C	Q00578
DNA-directed RNA polymerase II subunit RPB1	A	protein	1733	Saccharomyces cerevisiae S288C	P04050
DNA-directed RNA polymerase II subunit RPB2	B	protein	1224	Saccharomyces cerevisiae S288C	P08518
DNA-directed RNA polymerase II subunit RPB3	C	protein	347	Saccharomyces cerevisiae S288C	P16370
DNA-directed RNA polymerase II subunit RPB4	D	protein	221	Saccharomyces cerevisiae S288C	P20433

18 more molecules are not listed.

Sequence of entity 1 (0), FASTA

>7O72_1 General transcription and DNA repair factor IIH helicase subunit XPD (chains 0)
MKFYIDDLPVLFPYPKIYPEQYNYMCDIKKTLDVGGNSILEMPSGTGKTVSLLSLTIAYQ
MHYPEHRKIIYCSRTMSEIEKALVELENLMDYRTKELGYQEDFRGLGLTSRKNLCLHPEV
SKERKGTVVDEKCRRMTNGQAKRKLEEDPEANVELCEYHENLYNIEVEDYLPKGVFSFEK
LLKYCEEKTLCPYFIVRRMISLCNIIIYSYHYLLDPKIAERVSNEVSKDSIVIFDEAHNI
DNVCIESLSLDLTTDALRRATRGANALDERISEVRKVDSQKLQDEYEKLVQGLHSADILT
DQEEPFVETPVLPQDLLTEAIPGNIRRAEHFVSFLKRLIEYLKTRMKVLHVISETPKSFL
QHLKQLTFIERKPLRFCSERLSLLVRTLEVTEVEDFTALKDIATFATLISTYEEGFLLII
EPYEIENAAVPNPIMRFTCLDASIAIKPVFERFSSVIITSGTISPLDMYPRMLNFKTVLQ
KSYAMTLAKKSFLPMIITKGSDQVAISSRFEIRNDPSIVRNYGSMLVEFAKITPDGMVVF
FPSYLYMESIVSMWQTMGILDEVWKHKLILVETPDAQETSLALETYRKACSNGRGAILLS
VARGKVSEGIDFDHQYGRTVLMIGIPFQYTESRILKARLEFMRENYRIRENDFLSFDAMR
HAAQCLGRVLRGKDDYGVMVLADRRFSRKRSQLPKWIAQGLSDADLNLSTDMAISNTKQF
LRTMAQPTDPKDQEGVSVWSYEDLIKHQNSRKDQGGFIENENKEGEQDEDEDEDIEMQ

Sequence of entity 2 (1), FASTA

>7O72_2 General transcription and DNA repair factor IIH subunit TFB1 (chains 1)
GGSMSHSGAAIFEKVSGIIAINEDVSPAELTWRSTDGDKVHTVVLSTIDKLQATPASSEK
MMLRLIGKVDESKKRKDNEGNEVVPKPQRHMFSFNNRTVMDNIKMTLQQIISRYKDADIY
EEKRRREESAQHTETPMSSSSVTAGTPTPHLDTPQLNNGAPLINTAKLDDSLSKEKLLTN
LKLQQSLLKGNKVLMKVFQETVINAGLPPSEFWSTRIPLLRAFALSTSQKVGPYNVLSTI
KPVASSENKVNVNLSREKILNIFENYPIVKKAYTDNVPKNFKEPEFWARFFSSKLFRKLR
GEKIMQNDRGDVIIDRYLTLDQEFDRKDDDMLLHPVKKIIDLDGNIQDDPVVRGNRPDFT
MQPGVDINGNSDGTVDILKGMNRLSEKMIMALKNEYSRTNLQNKSNITNDEEDEDNDERN
ELKIDDLNESYKTNYAIIHLKRNAHEKTTDNDAKSSADSIKNADLKVSNQQMLQQLSLVM
DNLINKLDLNQVVPNNEVSNKINKRVITAIKINAKQAKHNNVNSALGSFVDNTSQANELE
VKSTLPIDLLESCRMLHTTCCEFLKHFYIHFQSGEQKQASTVKKLYNHLKDCIEKLNELF
QDVLNGDGESMSNTCTAYLKPVLNSITLATHKYDEYFNEYNNNSN

Sequence of entity 3 (2), FASTA

>7O72_3 General transcription and DNA repair factor IIH subunit TFB2 (chains 2)
GPGSMSDYSLKHSVTQYLEEIPQQVQNRLYTSPATCLAIYRILPPLAKFFIMAMVFNENE
VPLLDLDKWVNSNGKLQFQNAIKSMKSLHLLIPNKSSGTLMINLNPTFKISLRNALTGGE
VQNSFGVVVEENVVSLDLLDEYSANKWETILHFMVGTPLAKIPSEKVLNLLKHSKLMEEV
NSTGEFKITNEGFQFLLQEINSQLWTLLLQYLKMIETSKMDLVDVLHFIFMLGALEVGKA
YKIDALSETQRIMLQDMRDYGLVFQKHSNDSIFYPTKLALMLTSDTKTIRSASNAMDSVL
RQNREEPSVNEDGANGKSTTDITTSDDLNKAGLKNQDIPDGSLIVETNFKIYSYSNSPLQ
IAVLSLFVHLKARFVNMVLGQITRESIRRALTNGITADQIIAYLETHAHPQMRRLAEEKL
EKKLELDPNCKEPLQVLPPTVVDQIRLWQLELDRVITYEGSLYSDFETSQEYNLLSKYAQ
DIGVLLWKDDKKKKFFISKEGNSQVLDFAKRKLKKKQ

Sequence of entity 4 (3), FASTA

>7O72_4 RNA polymerase II transcription factor B subunit 3 (chains 3)
GPHMLMDEYEENKDMCPICKTDRYLSPDVKFLVNPECYHRICESCVDRIFSLGPAQCPYK
GCDKILRKNKFKTQIFDDVEVEKEVDIRKRVFNVFNKTIDDFNGDLVEYNKYLEEVEDII
YKLDHGIDVAKTEEKLRTYEELNKQLIMNNLERSRTEIESFEQRQKFEKEMKLKKRLLER
QIEEEERMNKEWTKKEIVNRLSTTTQDINETIEGVKNTVKLKKSSARRKLEELNRVLKNN
PYFNSNVNVQNSRLKDAVPFTPFNGDREAHPRFTLKGSVYNDPFIKDLEHRKEFIASGFN
TNYAYERVLTEAFMGLGCVISEEL

Sequence of entity 5 (4), FASTA

>7O72_5 General transcription and DNA repair factor IIH subunit TFB4 (chains 4)
SNAMDAISDPTFKHARSRKQVTEESPSLLTVIIEIAPKLWTTFDEEGNEKGSIIKVLEAL
IVFLNAHLAFNSANKVAVIAAYSQGIKYLYPESTSALKASESENKTRSDLKIINSDMYRR
FRNVDETLVEEIYKLFELEKKQIEQNSQRSTLAGAMSAGLTYVNRISKESVTTSLKSRLL
VLTCGSGSSKDEIFQYIPIMNCIFSATKMKCPIDVVKIGGSKESTFLQQTTDATNGVYLH
VESTEGLIQYLATAMFIDPSLRPIIVKPNHGSVDFRTSCYLTGRVVAVGFICSVCLCVLS
IIPPGNKCPACDSQFDEHVIAKLKRKPVVPRLKAKKKVTKP

Sequence of entity 6 (5), FASTA

>7O72_6 General transcription and DNA repair factor IIH subunit TFB5 (chains 5)
GPGSMARARKGALVQCDPSIKALILQIDAKMSDIVLEELDDTHLLVNPSKVEFVKHELNR
LLSKNIYNPMDEEENQ

Sequence of entity 7 (6), FASTA

>7O72_7 General transcription and DNA repair factor IIH subunit SSL1 (chains 6)
GGSMAPVVISESEEDEDRVAITRRTKRQVHFDGEGDDRVDQQQQQHSSSHRDRDKHVQRK
KKKRLSNRNLQGSNGGYAWEDEIKRSWDLVKVDDEGDMASLVASIVEARKKRTAKKNITP
YQRGIIRSLILTLDCSEAMLEKDLRPNRHAMIIQYAIDFVHEFFDQNPISQMGIIIMRNG
LAQLVSQVSGNPQDHIDALKSIRKQEPKGNPSLQNALEMARGLLLPVPAHCTREVLIVFG
SLSTTDPGDIHQTIDSLVSEKIRVKVLGLSAQVAICKELCKATNYGDESFYKILLDETHL
KELFNEAVTPLPVNKINKGFTLVKMGFPTRIFEDTPTFCSCHSKLVYGGYFCPNCHSKVC
SLPTVCPCCDLMLILSTHLARSYHHLMPLKTFAEVPTTEKFRSEDCFSCQSRFPILKNHK
NGKLLTSSRYRCEDCKQEFCVDCDVFIHEILHNCPGCESKPVIT

Sequence of entity 8 (7), FASTA

>7O72_8 General transcription and DNA repair factor IIH helicase subunit XPB (chains 7)
MTDVEGYQPKSKGKIFPDMGESFFSSDEDSPATDAEIDENYDDNRETSEGRGERDTGAMV
TGLKKPRKKTKSSRHTAADSSMNQMDAKDKALLQDTNSDIPADFVPDSVSGMFRSHDFSY
LRLRPDHASRPLWISPSDGRIILESFSPLAEQAQDFLVTIAEPISRPSHIHEYKITAYSL
YAAVSVGLETDDIISVLDRLSKVPVAESIINFIKGATISYGKVKLVIKHNRYFVETTQAD
ILQMLLNDSVIGPLRIDSDHQVQPPEDVLQQQLQQTAGKPATNVNPNDVEAVFSAVIGGD
NEREEEDDDIDAVHSFEIANESVEVVKKRCQEIDYPVLEEYDFRNDHRNPDLDIDLKPST
QIRPYQEKSLSKMFGNGRARSGIIVLPCGAGKTLVGITAACTIKKSVIVLCTSSVSVMQW
RQQFLQWCTLQPENCAVFTSDNKEMFQTESGLVVSTYSMVANTRNRSHDSQKVMDFLTGR
EWGFIILDEVHVVPAAMFRRVVSTIAAHAKLGLTATLVREDDKIGDLNFLIGPKLYEANW
MELSQKGHIANVQCAEVWCPMTAEFYQEYLRETARKRMLLYIMNPTKFQACQFLIQYHER
RGDKIIVFSDNVYALQEYALKMGKPFIYGSTPQQERMNILQNFQYNDQINTIFLSKVGDT
SIDLPEATCLIQISSHYGSRRQEAQRLGRILRAKRRNDEGFNAFFYSLVSKDTQEMYYST
KRQAFLVDQGYAFKVITHLHGMENIPNLAYASPRERRELLQEVLLKNEEAAGIEVGDDAD
NSVGRGSNGHKRFKSKAVRGEGSLSGLAGGEDMAYMEYSTNKNKELKEHHPLIRKMYYKN
LKK

Sequence of entity 9 (A), FASTA

>7O72_9 DNA-directed RNA polymerase II subunit RPB1 (chains A)
MVGQQYSSAPLRTVKEVQFGLFSPEEVRAISVAKIRFPETMDETQTRAKIGGLNDPRLGS
IDRNLKCQTCQEGMNECPGHFGHIDLAKPVFHVGFIAKIKKVCECVCMHCGKLLLDEHNE
LMRQALAIKDSKKRFAAIWTLCKTKMVCETDVPSEDDPTQLVSRGGCGNTQPTIRKDGLK
LVGSWKKDRATGDADEPELRVLSTEEILNIFKHISVKDFTSLGFNEVFSRPEWMILTCLP
VPPPPVRPSISFNESQRGEDDLTFKLADILKANISLETLEHNGAPHHAIEEAESLLQFHV
ATYMDNDIAGQPQALQKSGRPVKSIRARLKGKEGRIRGNLMGKRVDFSARTVISGDPNLE
LDQVGVPKSIAKTLTYPEVVTPYNIDRLTQLVRNGPNEHPGAKYVIRDSGDRIDLRYSKR
AGDIQLQYGWKVERHIMDNDPVLFNRQPSLHKMSMMAHRVKVIPYSTFRLNLSVTSPYNA
DFDGDEMNLHVPQSEETRAELSQLCAVPLQIVSPQSNKPCMGIVQDTLCGIRKLTLRDTF
IELDQVLNMLYWVPDWDGVIPTPAIIKPKPLWSGKQILSVAIPNGIHLQRFDEGTTLLSP
KDNGMLIIDGQIIFGVVEKKTVGSSNGGLIHVVTREKGPQVCAKLFGNIQKVVNFWLLHN
GFSTGIGDTIADGPTMREITETIAEAKKKVLDVTKEAQANLLTAKHGMTLRESFEDNVVR
FLNEARDKAGRLAEVNLKDLNNVKQMVMAGSKGSFINIAQMSACVGQQSVEGKRIAFGFV
DRTLPHFSKDDYSPESKGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVKTAETGYIQRR
LVKALEDIMVHYDNTTRNSLGNVIQFIYGEDGMDAAHIEKQSLDTIGGSDAAFEKRYRVD
LLNTDHTLDPSLLESGSEILGDLKLQVLLDEEYKQLVKDRKFLREVFVDGEANWPLPVNI
RRIIQNAQQTFHIDHTKPSDLTIKDIVLGVKDLQENLLVLRGKNEIIQNAQRDAVTLFCC
LLRSRLATRRVLQEYRLTKQAFDWVLSNIEAQFLRSVVHPGEMVGVLAAQSIGEPATQMT
LNTFHFAGVASKKVTSGVPRLKEILNVAKNMKTPSLTVYLEPGHAADQEQAKLIRSAIEH
TTLKSVTIASEIYYDPDPRSTVIPEDEEIIQLHFSLLDEEAEQSFDQQSPWLLRLELDRA
AMNDKDLTMGQVGERIKQTFKNDLFVIWSEDNDEKLIIRCRVVRPKSLDAETEAEEDHML
KKIENTMLENITLRGVENIERVVMMKYDRKVPSPTGEYVKEPEWVLETDGVNLSEVMTVP
GIDPTRIYTNSFIDIMEVLGIEAGRAALYKEVYNVIASDGSYVNYRHMALLVDVMTTQGG
LTSVTRHGFNRSNTGALMRCSFEETVEILFEAGASAELDDCRGVSENVILGQMAPIGTGA
FDVMIDEESLVKYMPEQKITEIEDGQDGGVTPYSNESGLVNADLDVKDELMFSPLVDSGS
NDAMAGGFTAYGGADYGEATSPFGAYGEAPTSPGFGVSSPGFSPTSPTYSPTSPAYSPTS
PSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYS
PTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPSYSPTSPSYSPTSP
SYSPTSPSYSPTSPNYSPTSPSYSPTSPGYSPGSPAYSPKQDEQKHNENENSR

Sequence of entity 10 (B), FASTA

>7O72_10 DNA-directed RNA polymerase II subunit RPB2 (chains B)
MSDLANSEKYYDEDPYGFEDESAPITAEDSWAVISAFFREKGLVSQQLDSFNQFVDYTLQ
DIICEDSTLILEQLAQHTTESDNISRKYEISFGKIYVTKPMVNESDGVTHALYPQEARLR
NLTYSSGLFVDVKKRTYEAIDVPGRELKYELIAEESEDDSESGKVFIGRLPIMLRSKNCY
LSEATESDLYKLKECPFDMGGYFIINGSEKVLIAQERSAGNIVQVFKKAAPSPISHVAEI
RSALEKGSRFISTLQVKLYGREGSSARTIKATLPYIKQDIPIVIIFRALGIIPDGEILEH
ICYDVNDWQMLEMLKPCVEDGFVIQDRETALDFIGRRGTALGIKKEKRIQYAKDILQKEF
LPHITQLEGFESRKAFFLGYMINRLLLCALDRKDQDDRDHFGKKRLDLAGPLLAQLFKTL
FKKLTKDIFRYMQRTVEEAHDFNMKLAINAKTITSGLKYALATGNWGEQKKAMSSRAGVS
QVLNRYTYSSTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGLVCPAETPEGQACGLVKNLS
LMSCISVGTDPMPIITFLSEWGMEPLEDYVPHQSPDATRVFVNGVWHGVHRNPARLMETL
RTLRRKGDINPEVSMIRDIREKELKIFTDAGRVYRPLFIVEDDESLGHKELKVRKGHIAK
LMATEYQDIEGGFEDVEEYTWSSLLNEGLVEYIDAEEEESILIAMQPEDLEPAEANEEND
LDVDPAKRIRVSHHATTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQAMGV
FLTNYNVRMDTMANILYYPQKPLGTTRAMEYLKFRELPAGQNAIVAIACYSGYNQEDSMI
MNQSSIDRGLFRSLFFRSYMDQEKKYGMSITETFEKPQRTNTLRMKHGTYDKLDDDGLIA
PGVRVSGEDVIIGKTTPISPDEEELGQRTAYHSKRDASTPLRSTENGIVDQVLVTTNQDG
LKFVKVRVRTTKIPQIGDKFASRHGQKGTIGITYRREDMPFTAEGIVPDLIINPHAIPSR
MTVAHLIECLLSKVAALSGNEGDASPFTDITVEGISKLLREHGYQSRGFEVMYNGHTGKK
LMAQIFFGPTYYQRLRHMVDDKIHARARGPMQVLTRQPVEGRSRDGGLRFGEMERDCMIA
HGAASFLKERLMEASDAFRVHICGICGLMTVIAKLNHNQFECKGCDNKIDIYQIHIPYAA
KLLFQELMAMNITPRLYTDRSRDF

Sequence of entity 11 (C), FASTA

>7O72_11 DNA-directed RNA polymerase II subunit RPB3 (chains C)
MGSHHHHHHSNSGLNDIFEAQKIEWHEDTGSSEGPQVKIREASKDNVDFILSNVDLAMAN
SLRRVMIAEIPTLAIDSVEVETNTTVLADEFIAHRLGLIPLQSMDIEQLEYSRDCFCEDH
CDKCSVVLTLQAFGESESTTNVYSKDLVIVSNLMGRNIGHPIIQDKEGNGVLICKLRKGQ
ELKLTCVAKKGIAKEHAKWGPAAAIEFEYDPWNKLKHTDYWYEQDSAKEWPQSKNCEYED
PPNEGDPFDYKAQADTFYMNVESVGSIPVDQVVVRGIDTLQKKVASILLALTQMDQDKVN
FASGDNNTASNMLGSNEDVMMTGAEQDPYSNASQMGNTGSGGYDNAW

Sequence of entity 12 (D), FASTA

>7O72_12 DNA-directed RNA polymerase II subunit RPB4 (chains D)
MNVSTSTFQTRRRRLKKVEEEENAATLQLGQEFQLKQINHQGEEEELIALNLSEARLVIK
EALVERRRAFKRSQKKHKKKHLKHENANDETTAVEDEDDDLDEDDVNADDDDFMHSETRE
KELESIDVLLEQTTGGNNKDLKNTMQYLTNFSRFRDQETVGAVIQLLKSTGLHPFEVAQL
GSLACDTADEAKTLIPSLNNKISDDELERILKELSNLETLY

Ligands and cofactors

ID	Name	Formula	Copies
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	1
MG	Magnesium ion	Mg	2
BEF	Beryllium trifluoride ion	Be F3	1
ZN	Zinc ion	Zn	17
SF4	Iron/sulfur cluster	Fe4 S4	1

Primary citation

Structure of RNA polymerase II pre-initiation complex at 2.9 angstrom defines initial DNA opening. Schilbach, S., Aibara, S., Dienemann, C. et al. Cell (2021) 184:4064-4072.e28. DOI 10.1016/j.cell.2021.05.012 · PubMed

Other PDB entries of the same protein (UniProt P06839 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7O4J 2.9 Å, Yeast RNA polymerase II transcription pre-initiation complex (consensus)
7ML0 3.0 Å, RNA polymerase II pre-initiation complex (PIC1)
8CEN 3.0 Å, Yeast RNA polymerase II transcription pre-initiation complex with core Mediator
7ML4 3.1 Å, RNA polymerase II initially transcribing complex (ITC)
7ZS9 3.1 Å, Yeast RNA polymerase II transcription pre-initiation complex with the +1 nucleosome…
7O4I 3.2 Å, Yeast RNA polymerase II transcription pre-initiation complex with initial transcription…
7O75 3.2 Å, Yeast RNA polymerase II transcription pre-initiation complex with open promoter DNA
7ML2 3.4 Å, RNA polymerase II pre-initiation complex (PIC3)
7O4L 3.4 Å, Yeast TFIIH in the expanded state within the pre-initiation complex
7O73 3.4 Å, Yeast RNA polymerase II transcription pre-initiation complex with closed distorted…
7O4K 3.6 Å, Yeast TFIIH in the contracted state within the pre-initiation complex
8CEO 3.6 Å, Yeast RNA polymerase II transcription pre-initiation complex with core Mediator and the…

Browse structure collections

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