7P8I: Processed angiotensin-converting enzyme 2

Receptor-binding domain (RBD) of the spike protein of the bat coronavirus RaTG13 virus in complex with the extracellular domain of human angiotensin-converting enzyme 2 (ACE2) - Crystal form 1. Determined by X-ray diffraction at 4.5 Å resolution. Released 3 Aug 2022.

Method

X-ray diffraction

Resolution

4.5 Å

Organisms

Homo sapiens, Bat coronavirus RaTG13

Chains

4

Atoms

13,101

Mol. weight

196.26 kDa

Ligands

NAG

Released

3 Aug 2022

Explore 7P8I in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7P8I contains 90 α-helices and 49 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 38 helices, 9 β-strands

Molecules and chains

>7P8I_1 Processed angiotensin-converting enzyme 2 (chains A, C)
GSSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQ
STLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPD
NPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHY
EDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYI
SPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFV
SVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEM
GHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEI
NFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDET
YCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFN
MLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADA
AA

>7P8I_2 Spike glycoprotein (chains B, D)
GSRVQPTDSIVRFPNITNLCPFGEVFNATTFASVYAWNRKRISNCVADYSVLYNSTSFST
FKCYGVSPTKLNDLCFTNVYADSFVITGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAW
NSKHIDAKEGGNFNYLYRLFRKANLKPFERDISTEIYQAGSKPCNGQTGLNCYYPLYRYG
FYPTDGVGHQPYRVVVLSFELLNAPATVCGPKKSTNLVKNKCVNFAAHHHHHH

Ligands and cofactors

Primary citation

Evidence of SARS-CoV-2 Direct Evolution in R. affinis Bats Driven by Affinity and Dynamics Optimization of the Spike Protein. Castelli, M., Scietti, L., Faravelli, S. et al. To be published.

Related structures

Other PDB entries of the same protein (UniProt Q9BYF1 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 9SPA 1.79 Å, Apo ACE2 extracellular domain
• 28KD 2.02 Å, ACE2 extracellular domain in complex with the macrocyclic peptide GR3.1.2
• 8BYJ 2.07 Å, The structures of Ace2 in complex with bicyclic peptide inhibitor
• 8B9P 2.11 Å, ACE2 in complex with bicyclic peptide inhibitor
• 8XSF 2.16 Å, SARS-CoV-2 rbd + imcas-364 + hACE2
• 1R42 2.2 Å, Native Human Angiotensin Converting Enzyme-Related Carboxypeptidase (ACE2)
• 7JVO 2.2 Å, Importin alpha bound to the C-terminus of ACE2
• 8TOR 2.2 Å, ACE2-peptide 2 complex
• 9RVA 2.2 Å, Crystal structure of the extracellular part of human ACE2 in complex with the…
• 9UE7 2.27 Å, Cryo-EM structure of SARS-CoV-2 KP.2 spike in complex with ACE2
• 8TOQ 2.3 Å, ACE2-peptide 1 complex
• 8BFW 2.33 Å, The structures of Ace2 in complex with bicyclic peptide inhibitor

Browse structure collections

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