Cryo-EM structure of murine Dispatched NNN mutant. Determined by electron microscopy at 3.2 Å resolution. Released 27 Oct 2021.
Explore 7RPJ in 3D Show helices and sheets RCSB PDB PDBe
7RPJ contains 48 α-helices and 24 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 180-186 | 7 | |
| α-helix | 188-208 | 21 | |
| α-helix | 211-213 | 3 | |
| β-strand | 224 | 1 | 1 |
| α-helix | 228-242 | 15 | |
| β-strand | 250 | 1 | 2 |
| α-helix | 256-262 | 7 | |
| β-strand | 299-305 | 7 | 3 |
| α-helix | 315-325 | 11 | |
| α-helix | 326-330 | 5 | |
| α-helix | 335-338 | 4 | |
| β-strand | 351 | 1 | 3 |
| α-helix | 353-360 | 8 | |
| α-helix | 366-368 | 3 | |
| α-helix | 371-390 | 20 | |
| α-helix | 415-418 | 4 | |
| α-helix | 422-425 | 4 | |
| α-helix | 426-430 | 5 | |
| β-strand | 431 | 1 | 3 |
| β-strand | 432 | 1 | 4 |
| β-strand | 451-458 | 8 | 3 |
| α-helix | 461-463 | 3 | |
| α-helix | 464-466 | 3 | |
| α-helix | 467-471 | 5 | |
| β-strand | 477 | 1 | 3 |
| β-strand | 482-487 | 6 | 3 |
| α-helix | 495-502 | 8 | |
| α-helix | 504-521 | 18 | |
| α-helix | 525-543 | 19 | |
| α-helix | 544-548 | 5 | |
| α-helix | 557-586 | 30 | |
| α-helix | 592-620 | 29 | |
| α-helix | 621-623 | 3 | |
| β-strand | 626 | 1 | 1 |
| α-helix | 627-661 | 35 | |
| α-helix | 662-664 | 3 | |
| α-helix | 682-702 | 21 | |
| α-helix | 703-707 | 5 | |
| α-helix | 708-714 | 7 | |
| α-helix | 716-735 | 20 | |
| α-helix | 747-749 | 3 | |
| β-strand | 753 | 1 | 5 |
| α-helix | 758-764 | 7 | |
| α-helix | 766-768 | 3 | |
| β-strand | 770 | 1 | 4 |
| α-helix | 771-776 | 6 | |
| β-strand | 782-789 | 8 | 2 |
| β-strand | 791 | 1 | 6 |
| β-strand | 810 | 1 | 6 |
| α-helix | 819-834 | 16 | |
| β-strand | 838 | 1 | 7 |
| α-helix | 850-860 | 11 | |
| α-helix | 881-894 | 14 | |
| β-strand | 909-912 | 4 | 2 |
| β-strand | 918-926 | 9 | 2 |
| β-strand | 927 | 1 | 7 |
| β-strand | 928-930 | 3 | 2 |
| α-helix | 935-953 | 19 | |
| α-helix | 956-957 | 2 | |
| α-helix | 960-962 | 3 | |
| β-strand | 964-966 | 3 | 2 |
| α-helix | 970-1001 | 32 | |
| β-strand | 1003 | 1 | 8 |
| α-helix | 1004-1027 | 24 | |
| α-helix | 1034-1060 | 27 | |
| α-helix | 1066-1094 | 29 | |
| β-strand | 1100 | 1 | 5 |
| α-helix | 1101-1119 | 19 | |
| α-helix | 1120-1124 | 5 | |
| α-helix | 1125-1132 | 8 | |
| α-helix | 1137-1139 | 3 | |
| β-strand | 1140 | 1 | 8 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Protein dispatched homolog 1 | A | protein | 1352 | Mus musculus | Q3TDN0 (AlphaFold model) |
>7RPJ_1 Protein dispatched homolog 1 (chains A) MARPFKFPRSYAALLADWPVVVLGMCTLLIVVCALVGVLVPELPDFSDPLLGFEPRGTTI GQRLVTWNNMMRNTGYKATLANYPYKYAEEQARSHRDDRWSDDHHERERREVDWNFQKDS FFCDVPSDGYSRVVFASAGGETLWNLPAIKSMCDVDNSRIRSHPQFSDLCQRTTAVSCCP SWTLGNYIAILNNRSSCQKIVERDVSHTLKLLRTCAKHYQNGTLGPDCWDKAARRKDQLK CTNVPRKCTKYNAVYQILHYLVDKDFMTPKTADYAVPALKYSMLFSPTEKGESMMNIYLD NFENWNSSDGITTVTGIEFGIKHSLFQDYLLMDTVYPAIAIAIVLLIMCVYTKSMFITLM TMFAIISSLIVSYFLYRVVFNFEFFPFMNLTALIILVGIGANNAFVLCDVWNYTKFDKPR AETSEAVSVTLQHAALSMFVTSFTTAAAFYANYVSNITAIRCFGVYAGTAILVNYVLMVT WLPAVIVLHERYLLNIFTCFRKPQPQAYDKSCWAVLCQKCRRVLFAVSEASRIFFEKVLP CIVIKFRYLWLIWFLALTVGGAYIVCVNPKMKLPSLELSEFQVFRSSHPFERYDAEFKKL FMFERVHHGEELHMPITVIWGVSPEDSGDPLNPKSKGELTLDSTFNIASPASQAWILHFC QKLRNQTFFHQTEQQDFTSCFIETFKQWMENQDCDEPALYPCCSHCSFPYKQEVFELCIK KAIMELDRSTGYHLNNKTPGPRFDINDTIRAVVLEFQSTFLFTLAYEKMQQFYKEVDSWI SHELSSAPEGLSRGWFVSNLEFYDLQDSLSDGTLIAMGLSVAVAFSVMLLTTWNIIISLY AIVSIAGTIFVTVGSLVLLGWELNVLESVTISVAVGLSVNFAVHYGVAYRLAPDPDREGK VIFSLSRMGSAIAMAALTTFVAGAMMMPSTVLAYTQLGTFMMLVMCVSWAFATFFFQCLC RCLGPQGTCGQIPFPTKLQCSPFSHTLSARPGDRGPSKTHAASAYSVDARGQKSQLEHEF YELQPLASHSCTSSEKTTYEEPHTCSEFFNGQAKNLRMPVPAAYSSELTKSPSSEPGSAL LQSCLEQDTVCHFSLNPRCNCRDAYTHLQYGLPEIHCQQMGDSLCHKCASTAGGFVQIQS SVAPLKASHQAAEGLLHPAQHMLPPGMQNSRPRNFFLHSVQHFQAQENLGRTSTHSTDER LPRTAELSPPPSDSRSTESFQRACCHPENNQRRLCKSRDPGDTEGSGGTKSKVSGLPNQT DKEEKQVEPSLLQTDETVNSEHLNHNESNFTFSHLPGEAGCRSCPNSPQSCRSIMRSKCG TEDCQTPNLEANVPAVPTHSDLSGESLLIKTL
| ID | Name | Formula | Copies |
|---|---|---|---|
| NAG | 2-acetamido-2-deoxy-beta-D-glucopyranose | C8 H15 N O6 | 5 |
| Y01 | Cholesterol hemisuccinate | C31 H50 O4 | 14 |
Dispatched uses Na + flux to power release of lipid-modified Hedgehog. Wang, Q., Asarnow, D.E., Ding, K. et al. Nature (2021) 599:320-324. DOI 10.1038/s41586-021-03996-0 · PubMed
Other PDB entries of the same protein (UniProt Q3TDN0 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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