7SKW: Lysozyme C

Ab initio structure of triclinic lysozyme from electron-counted MicroED data. Determined by electron crystallography at 0.87 Å resolution. Released 8 Jun 2022.

Method: Electron crystallography
Resolution: 0.87 Å
Organism: Gallus gallus
Chains: 1
Atoms: 1,190
Mol. weight: 14.58 kDa
Released: 8 Jun 2022

Explore 7SKW in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7SKW contains 7 α-helices and 9 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	2	1	1
α-helix	5-14	10
β-strand	20	1	2
β-strand	23	1	2
α-helix	25-36	12
β-strand	39	1	1
β-strand	43-45	3	3
β-strand	51-53	3	3
β-strand	58-59	2	3
β-strand	65	1	4
β-strand	79	1	4
α-helix	80-84	5
α-helix	89-100	12
α-helix	105-107	3
α-helix	109-114	6
α-helix	120-123	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Lysozyme C	A	protein	129	Gallus gallus	P00698 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7SKW_1 Lysozyme C (chains A)
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINS
RWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDV
QAWIRGCRL

Primary citation

Ab initio phasing macromolecular structures using electron-counted MicroED data. Martynowycz, M.W., Clabbers, M.T.B., Hattne, J. et al. Nat Methods (2022) 19:724-729. DOI 10.1038/s41592-022-01485-4 · PubMed

Other PDB entries of the same protein (UniProt P00698 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2VB1 0.65 Å, HEWL at 0.65 angstrom resolution
8ZST 0.8 Å, Lysozyme Crystallized with Bioassembler on Earth
9QUM 0.83 Å, Structure of lysozyme by continuous serial electron diffraction (SerialED)
7ULY 0.87 Å, MicroED structure of triclinic lysozyme
7P6M 0.89 Å, Hydrogenated refolded hen egg-white lysozyme
9RZL 0.9 Å, Hen egg-white lysozyme (HEWL)
8RLI 0.91 Å, Neutron structure of perdeuterated hen egg-white lysozyme at room temperature
7YRK 0.92 Å, Crystal structure of the hen egg lysozyme-2-oxidobenzylidene-threoninato-copper (II)…
3LZT 0.93 Å, Refinement of triclinic lysozyme at atomic resolution
1IEE 0.94 Å, Structure of tetragonal hen egg white lysozyme at 0.94 a from crystals grown by the…
7OL5 0.94 Å, Crystal structure of Lysozyme in complex with Hepes
4LZT 0.95 Å, Atomic resolution refinement of triclinic hew lysozyme at 295K

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