7TRF: Human telomerase catalytic core RNP with H2A/H2B

Human telomerase catalytic core RNP with H2A/H2B. Determined by electron microscopy at 3.7 Å resolution. Released 20 Apr 2022.

Method: Electron microscopy
Resolution: 3.7 Å
Organism: Homo sapiens
Chains: 5
Atoms: 13,581
Mol. weight: 309.69 kDa
Released: 20 Apr 2022

Explore 7TRF in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7TRF contains 57 α-helices and 40 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 48 helices, 36 β-strands

Element	Residues	Length	Sheet
α-helix	8-17	10
β-strand	21-23	3	3
α-helix	24-31	8
α-helix	45-53	9
β-strand	54-57	4	3
α-helix	63-65	3
α-helix	77-91	15
β-strand	101-103	3	4
β-strand	119-121	3	4
α-helix	126-133	8
α-helix	135-144	10
α-helix	147-155	9
β-strand	157-162	6	3
β-strand	166-171	6	3
α-helix	175-177	3
β-strand	325-327	3	5
α-helix	328-331	4
α-helix	346-349	4
α-helix	354-365	12
α-helix	379-383	5
α-helix	384-387	4
α-helix	390-400	11
α-helix	405-412	8
α-helix	447-453	7
β-strand	456	1	6
α-helix	458-470	13
α-helix	475-478	4
α-helix	481-495	15
β-strand	502-504	3	5
α-helix	505-508	4
α-helix	518-520	3
α-helix	531-547	17
α-helix	548-553	6
α-helix	554-560	7
β-strand	561-564	4	6
β-strand	574-577	4	6
α-helix	578-595	18
β-strand	598-600	3	7
α-helix	601-602	2
α-helix	603-611	9
β-strand	618-626	9	7
β-strand	629-636	8	7
α-helix	653-671	19
α-helix	673-676	4
β-strand	679	1	8
α-helix	683-697	15
α-helix	703-706	4
β-strand	707-713	7	8
α-helix	722-732	11
β-strand	738-739	2	9
β-strand	740-749	10	3
β-strand	755-764	10	3
α-helix	766-768	3
α-helix	773-783	11
β-strand	789-795	7	3
β-strand	800-801	2	9
α-helix	802-814	13
β-strand	817-820	4	7
β-strand	823-827	5	7
α-helix	838-853	16
β-strand	862-865	4	8
β-strand	869-874	6	8
α-helix	877-889	13
β-strand	891	1	10
β-strand	896	1	10
β-strand	898-905	8	8
β-strand	920-921	2	8
β-strand	927-930	4	11
β-strand	933-936	4	11
β-strand	942-944	3	11
α-helix	947-949	3
β-strand	953	1	12
α-helix	955-957	3
α-helix	966-981	16
α-helix	984-987	4
α-helix	994-1016	23
α-helix	1025-1027	3
α-helix	1029-1050	22
β-strand	1058	1	13
β-strand	1062	1	13
α-helix	1067-1081	15
α-helix	1086-1106	21
α-helix	1109-1118	10
β-strand	1131	1	12

Chain E: 5 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	19-22	4
α-helix	28-37	10
β-strand	43-44	2	2
α-helix	47-73	27
β-strand	79	1	1
α-helix	82-90	9
α-helix	93-97	5

Chain F: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	39-49	11
β-strand	55	1	1
α-helix	57-85	29
β-strand	89-90	2	2
α-helix	92-102	11
α-helix	106-123	18

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Histone H2B type 1-C/E/F/G/I	F	protein	126	Homo sapiens	P62807 (AlphaFold model)
Histone H2A	E	protein	129	Homo sapiens	Q9BTM1 (AlphaFold model)
Telomerase RNA, partial sequence	B	RNA	451	Homo sapiens
Telomerase reverse transcriptase	A	protein	1167	Homo sapiens	O14746 (AlphaFold model)
Telomeric repeat substrate	D	DNA	18	Homo sapiens

Sequence of entity 1 (F), FASTA

>7TRF_1 Histone H2B type 1-C/E/F/G/I (chains F)
MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAM
GIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVT
KYTSSK

Sequence of entity 2 (E), FASTA

>7TRF_2 Histone H2A (chains E)
MSGRGKQGGKVRAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLT
AEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKK
TESQKTKSK

Sequence of entity 3 (B), FASTA

>7TRF_3 Telomerase RNA, partial sequence (chains B)
GGGUUGCGGAGGGUGGGCCUGGGAGGGGUGGUGGCCAUUUUUUGUCUAACCCUAACUGAG
AAGGGCGUAGGCGCCGUGCUUUUGCUCCCCGCGCGCUGUUUUUCUCGCUGACUUUCAGCG
GGCGGAAAAGCCUCGGCCUGCCGCCUUCCACCGUUCAUUCUAGAGCAAACAAAAAAUGUC
AGCUGCUGGCCCGUUCGCCCCUCCCGGGGACCUGCGGCGGGUCGCCUGCCCAGCCCCCGA
ACCCCGCCUGGAGGCCGCGGUCGGCCCGGGGCUUCUCCGGAGGCACCCACUGCCACCGCG
AAGAGUUGGGCUCUGUCAGCCGCGGGUCUCUCGGGGGCGAGGGCGAGGUUCAGGCCUUUC
AGGCCGCAGGAAGAGGAACGGAGCGAGUCCCCGCGCGCGGCGCGAUUCCCUGAGCUGUGG
GACGUGCACCCAGGACUCGGCUCACACAUGC

Sequence of entity 4 (A), FASTA

>7TRF_4 Telomerase reverse transcriptase (chains A)
GHMSAWSHPQFEKGGGSGGGSGGSAWSHPQFEKSAMPRAPRCRAVRSLLRSHYREVLPLA
TFVRRLGPQGWRLVQRGDPAAFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVL
QRLCERGAKNVLAFGFALLDGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVG
DDVLVHLLARCALFVLVAPSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNH
SVREAGVPLGLPAPGARRRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGP
SDRGFCVVSPARPAEEATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVY
AETKHFLYSSGDKEQLRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQR
YWQMRPLFLELLGNHAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDP
RRLVQLLRQHSSPWQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSL
QELTWKMSVRDCAWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTE
TTFQKNRLFFYRKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIP
KPDGLRPIVNMDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDD
IHRAWRTFVLRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYA
VVQKAAHGHVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGL
FDVFLRFMCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLR
LVDDFLLVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAH
GLFPWCGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSL
FLDLQVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYS
ILKAKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSR
KLPGTTLTALEAAANPALPSDFKTILD

Sequence of entity 5 (D), FASTA

>7TRF_5 Telomeric repeat substrate (chains D)
TTTTTTTTTTTTTTAGGG

Primary citation

Structure of active human telomerase with telomere shelterin protein TPP1. Liu, B., He, Y., Wang, Y. et al. Nature (2022) 604:578-583. DOI 10.1038/s41586-022-04582-8 · PubMed

Other PDB entries of the same protein (UniProt P62807 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6ACO 1.71 Å, histone lysine desuccinylase Sirt5 in complex with succinyl peptide H2BK120
7R5R 2.44 Å, Structure of the human CCAN CENP-A alpha-satellite complex
10GL 2.5 Å, Nucleosome containing an 8oxoG:A at SHL+3
10GM 2.5 Å, Nucleosome containing an 8oxoG:A at SHL+2
8OX0 2.52 Å, Structure of apo telomeric nucleosome
9Y46 2.59 Å, Human nucleosome structure on Nickel-NTA lipid affinity grid (C2 refinement)
10XZ 2.6 Å, Non-damaged nucleosome
7PII 2.68 Å, Structure of the human CCAN CENP-A alpha-satellite complex
7U46 2.68 Å, Cryo-EM structure of CENP-A nucleosome (palindromic alpha satellite DNA) in complex with…
10GK 2.7 Å, Nucleosome containing an 8oxoG:A at SHL+4
10YA 2.7 Å, Nucleosome containing a nick at SHL0
10YC 2.7 Å, Nucleosome containing a nick at SHL-4

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