7TRY: Corticotropin releasing factor receptor 2

Cryo-EM structure of corticotropin releasing factor receptor 2 bound to Urocortin 1 and coupled with heterotrimeric G11 protein. Determined by electron microscopy at 3.7 Å resolution. Released 9 Nov 2022.

Method: Electron microscopy
Resolution: 3.7 Å
Organisms: Homo sapiens, Rattus norvegicus, synthetic construct
Chains: 6
Atoms: 8,340
Mol. weight: 187.68 kDa
Released: 9 Nov 2022

Explore 7TRY in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7TRY contains 43 α-helices and 70 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	13-38	26
β-strand	39-45	7	6
α-helix	48-50	3
α-helix	52-60	9
β-strand	190-195	6	6
β-strand	200-205	6	6
α-helix	213-215	3
α-helix	217-220	4
β-strand	225-231	7	6
α-helix	234-236	3
β-strand	238	1	7
β-strand	246	1	7
α-helix	247-259	13
β-strand	268-274	7	6
α-helix	276-285	10
α-helix	302-314	13
β-strand	324-328	5	6
α-helix	336-355	20

Chain B: 5 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-32	3
α-helix	34-36	3
β-strand	47-52	6	8
β-strand	58-63	6	9
β-strand	69-74	6	9
β-strand	78-83	6	9
β-strand	89-94	6	9
α-helix	95	1
β-strand	100-105	6	10
β-strand	111-116	6	10
β-strand	120-125	6	10
β-strand	135-140	6	10
β-strand	146-151	6	11
β-strand	156-161	6	11
β-strand	166-170	5	11
β-strand	176-180	5	11
β-strand	187-192	6	12
β-strand	198-203	6	12
β-strand	208-212	5	12
β-strand	218-222	5	12
β-strand	229-234	6	13
β-strand	240-245	6	13
β-strand	250-254	5	13
β-strand	259-264	6	13
α-helix	272	1
β-strand	273-278	6	14
β-strand	284-289	6	14
β-strand	294-298	5	14
β-strand	304-308	5	14
β-strand	315-320	6	8
β-strand	327-331	5	8
β-strand	335-339	5	8

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	8-23	16
α-helix	30-43	14
α-helix	45-47	3

Chain H: 7 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	15
β-strand	10-12	3	16
β-strand	18-25	8	15
α-helix	29-31	3
β-strand	33-39	7	16
β-strand	45-51	7	16
α-helix	53-55	3
β-strand	58-60	3	16
β-strand	68-73	6	15
β-strand	78-83	6	15
α-helix	88-90	3
β-strand	92-99	8	16
β-strand	111	1	16
β-strand	115-119	5	16
α-helix	138-139	2
β-strand	140-141	2	17
β-strand	146-147	2	18
β-strand	155-161	7	17
β-strand	166	1	19
β-strand	172	1	19
β-strand	174-179	6	18
β-strand	185-190	6	18
β-strand	194-195	2	18
α-helix	196	1
β-strand	203-207	5	17
β-strand	211-216	6	17
α-helix	221-223	3
β-strand	226-231	6	18
α-helix	237	1
β-strand	238-239	2	18
β-strand	243-245	3	18

Chain P: 16 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	44	1	1
α-helix	49	1
β-strand	50	1	1
α-helix	51-52	2
β-strand	62-63	2	2
α-helix	64-65	2
β-strand	67-68	2	3
β-strand	71-72	2	3
β-strand	73	1	4
β-strand	78-79	2	2
β-strand	80-83	4	5
β-strand	89-94	6	5
β-strand	100	1	4
α-helix	110-140	31
α-helix	147-168	22
α-helix	174-178	5
α-helix	182-214	33
α-helix	216-221	6
α-helix	224-232	9
α-helix	235-249	15
α-helix	266-297	32
α-helix	301-319	19
α-helix	322-325	4
α-helix	334-356	23
α-helix	357-361	5
α-helix	364-382	19

Chain U: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	6-8	3
α-helix	9-39	31

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Corticotropin-releasing factor receptor 2	P	protein	560	Homo sapiens	Q13324 (AlphaFold model)
Urocortin	U	protein	40	Homo sapiens	P55089 (AlphaFold model)
Guanine nucleotide-binding protein subunit alpha-11	A	protein	353	Homo sapiens	P29992 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	400	Rattus norvegicus	P54311 (AlphaFold model)
G protein gamma subunit	G	protein	71	Rattus norvegicus	A0A9X9ZA26
scFV16	H	protein	247	synthetic construct

Sequence of entity 1 (P), FASTA

>7TRY_1 Corticotropin-releasing factor receptor 2 (chains P)
DAALLHSLLEANCSLALAEELLLDGWGPPLDPEGPYSYCNTTLDQIGTCWPRSAAGALVE
RPCPEYFNGVKYNTTRNAYRECLENGTWASKINYSQCEPILDDKQRKYDLHYRIALVVNY
LGHCVSVAALVAAFLLFLALRSIRCLRNVIHWNLITTFILRNVMWFLLQLVDHEVHESNE
VWCRCITTIFNYFVVTNFFWMFVEGCYLHTAIVMTYSTERLRKCLFLFIGWCIPFPIIVA
WAIGKLYYENEQCWFGKEPGDLVDYIYQGPIILVLLINFVFLFNIVRILMTKLRASTTSE
TIQYRKAVKATLVLLPLLGITYMLFFVNPGEDDLSQIMFIYFNSFLQSFQGFFVSVFYCF
FNGEVRSAVRKRWHRWQDHHSLRVPMAGSSGGGGSGGGGSSGVFTLEDFVGDWEQTAAYN
LDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEEVF
KVVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKI
IDERLITPDGSMLFRVTINS

Sequence of entity 2 (U), FASTA

>7TRY_2 Urocortin (chains U)
DNPSLSIDLTFHLLRTLLELARTQSQRERAEQNRIIFDSV

Sequence of entity 3 (A), FASTA

>7TRY_3 Guanine nucleotide-binding protein subunit alpha-11 (chains A)
MGCTLSAEDKAAVERSKMIEKQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGAG
YSEEDKRGFTKLVYQNIFTAMQAMIRAMETLKILYKYEQNKANALLIREVDVEKVTTFEH
QYVSAIKTLWEDPGIQECYDRRREYQLSDSAKYYLTDVDRIATLGYLPTQQDVLRVRVPT
TGIIEYPFDLENIIFRMVDVGAQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNEN
RMEESKALFRTIITYPWFQNSSVILFLNKKDLLEDKILYSHLVDYFPEFDGPQRDAQAAR
EFILKMFVDLNPDSDKIIYSHFTCSTDTENIRFVFAAVKDTILQLNLKEYNLV

Sequence of entity 4 (B), FASTA

>7TRY_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHSSGLVPRGSHMASHHHHHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACA
DATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYT
TNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYL
SCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDA
SAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSH
DNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMA
VATGSWDSFLKIWNGSSGGGGSGGGGSSGVSGWRLFKKIS

Sequence of entity 5 (G), FASTA

>7TRY_5 G protein gamma subunit (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 6 (H), FASTA

>7TRY_6 scFV16 (chains H)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSA
GGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLEL

Primary citation

Structure insights into selective coupling of G protein subtypes by a class B G protein-coupled receptor. Zhao, L.H., Lin, J., Ji, S.Y. et al. Nat Commun (2022) 13:6670-6670. DOI 10.1038/s41467-022-33851-3 · PubMed

Other PDB entries of the same protein (UniProt Q13324 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3N93 2.5 Å, Crystal structure of human CRFR2 alpha extracellular domain in complex with Urocortin 3
3N95 2.72 Å, Crystal structure of human CRFR2 alpha extracellular domain in complex with Urocortin 2
3N96 2.75 Å, Crystal structure of human CRFR2 alpha extracellular domain in complex with Urocortin 1
6PB1 2.8 Å, Cryo-EM structure of Urocortin 1-bound Corticotropin-releasing factor 2 receptor in…
7TS0 2.8 Å, Cryo-EM structure of corticotropin releasing factor receptor 2 bound to Urocortin 1 and…

Browse structure collections

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