7VVK: PTH-bound human PTH1R

PTH-bound human PTH1R in complex with Gs (class1). Determined by electron microscopy at 3.3 Å resolution. Released 3 Aug 2022.

Method: Electron microscopy
Resolution: 3.3 Å
Organisms: Homo sapiens, Rattus norvegicus, Bos taurus
Chains: 6
Atoms: 8,516
Mol. weight: 163.95 kDa
Released: 3 Aug 2022

Explore 7VVK in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7VVK contains 39 α-helices and 58 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	14-39	26
β-strand	40-45	6	1
α-helix	53-57	5
β-strand	208-213	6	1
β-strand	218-223	6	1
α-helix	236-238	3
β-strand	243-249	7	1
α-helix	256-267	12
β-strand	276-282	7	1
α-helix	284-293	10
α-helix	298-301	4
α-helix	303-305	3
α-helix	316-317	2
α-helix	322-342	21
β-strand	349	1	1
β-strand	352-353	2	1
α-helix	363-380	18

Chain B: 4 helices, 32 β-strands

Element	Residues	Length	Sheet
α-helix	2-24	23
α-helix	30-33	4
α-helix	38-39	2
β-strand	47	1	2
β-strand	51	1	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
α-helix	95	1
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	121-125	5	5
β-strand	134-139	6	5
β-strand	146-148	3	6
β-strand	151	1	7
β-strand	157	1	7
β-strand	158-161	4	6
β-strand	166-168	3	6
β-strand	178-180	3	6
β-strand	187-192	6	8
β-strand	198-203	6	8
β-strand	207-212	6	8
β-strand	217-223	7	8
β-strand	229-234	6	9
β-strand	240-245	6	9
β-strand	250-254	5	9
β-strand	260-264	5	9
β-strand	273-278	6	10
β-strand	284-289	6	10
β-strand	294-298	5	10
β-strand	304-308	5	10
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336	1	3
β-strand	337-339	3	2

Chain G: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	9-23	15
α-helix	30-32	3
α-helix	34-43	10
α-helix	44-47	4
α-helix	53-55	3

Chain N: 1 helix, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-6	4	11
β-strand	11-12	2	12
β-strand	18-25	8	11
β-strand	34-39	6	13
β-strand	45-51	7	13
β-strand	58-60	3	13
β-strand	68-73	6	11
β-strand	78-83	6	11
α-helix	88-90	3
β-strand	92-98	7	13
β-strand	118	1	13
β-strand	122-124	3	13
β-strand	125-126	2	12

Chain P: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	3-33	31

Chain R: 18 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	34-48	15
β-strand	112	1	14
β-strand	117	1	14
β-strand	128-130	3	15
α-helix	131-132	2
β-strand	143-145	3	15
β-strand	148	1	16
β-strand	154	1	16
α-helix	155-156	2
β-strand	166	1	15
α-helix	181-211	31
α-helix	213-215	3
α-helix	218-240	23
α-helix	241-245	5
α-helix	279-311	33
α-helix	321-325	5
α-helix	326-330	5
α-helix	332-345	14
α-helix	357-360	4
α-helix	361-390	30
α-helix	399-417	19
α-helix	421-423	3
α-helix	435-448	14
α-helix	451-460	10
α-helix	464-477	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	380	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	351	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	68	Bos taurus	P63212 (AlphaFold model)
nanobody Nb35	N	protein	137	unidentified
Parathyroid hormone	P	protein	34	Homo sapiens	P01270 (AlphaFold model)
Parathyroid hormone/parathyroid hormone-related peptide receptor	R	protein	473	Homo sapiens	Q03431

Sequence of entity 1 (A), FASTA

>7VVK_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
GNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYH
VNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPEN
QFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDV
IKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAI
IFVVDSSDYNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPE
FARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENARR
IFNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>7VVK_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>7VVK_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFS

Sequence of entity 4 (N), FASTA

>7VVK_4 nanobody Nb35 (chains N)
MGQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASI
SYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYR
GQGTQVTVSSLHHHHHH

Sequence of entity 5 (P), FASTA

>7VVK_5 Parathyroid hormone (chains P)
SVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNF

Sequence of entity 6 (R), FASTA

>7VVK_6 Parathyroid hormone/parathyroid hormone-related peptide receptor (chains R)
DYKDDDDKDADDVMTKEEQIFLLHRAQAQCEKRLKEVLQRPASIMESDKGWTSASTSGKP
RKDKASGKLYPESEEDKEAPTGSRYRGRPCLPEWDHILCWPLGAPGEVVAVPCPDYIYDF
NHKGHAYRRCDRNGSWELVPGHNRTWANYSECVKFLTNETREREVFDRLGMIYTVGYSVS
LASLTVAVLILAYFRRLHCTRNYIHMHLFLSFMLRAVSIFVKDAVLYSGATLDEAERLTE
EELRAIAQAPPPPATAAAGYAGCRVAVTFFLYFLATNYYWILVEGLYLHSLIFMAFFSEK
KYLWGFTVFGWGLPAVFVAVWVSVRATLANTGCWDLSSGNKKWIIQVPILASIVLNFILF
INIVRVLATKLRETNAGRCDTRQQYRKLLKSTLVLMPLFGVHYIVFMATPYTEVSGTLWQ
VQMHYEMLFNSFQGFFVAIIYCFCNGEVQAEIKKSWSRWTLALDFKRKARSGS

Primary citation

Endogenous ligand recognition and structural transition of a human PTH receptor. Kobayashi, K., Kawakami, K., Kusakizako, T. et al. Mol Cell (2022) 82:3468-3483.e5. DOI 10.1016/j.molcel.2022.07.003 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

Browse structure collections

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